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Table 1

Specificities and performance of monoclonal antibodies detecting MDR transporter proteins

Transporter protein, Mab name, species, isotype, fusion protein used for immunization, and smallest reactive amino acid sequence known are indicated in the columns on the left. Reactivity in different techniques is indicated in the middle columns. The columns on the right show reactivities towards the respective antigens in frozen sections of different species. For reasons of comparison, the anti-MDR1 P-gp Mabs JSB-1 and C219 are included in the table.

Transporter proteinMabAntigenReactivitya
NameSpeciesIsotypeFusion proteinAmino acid sequenceCytospins/Frozen sectionsParaffin sectionsWestern blotFACSSpecies
HumanMouseRatGuinea Pig
MDR1 P-gp JSB1 Mouse IgG1 NAb ? +++ + + ++ +++ − − − 
 C219 Mouse IgG2a NA 569–74+ 1214–19 +++ ++ +++ ++ +++ ++ ++ ++ 
MDR3 P-gp P3II-1 Mouse IgG1 P3II  629–692 ++ +/− + ND ++ − +/− +/− 
 P3II-26 Mouse IgG2b P3II  629–692 +++ +/− +++ ND +++ +/− + ++ 
MRP1 MRPr1 Rat IgG2a M1I  238–247 +++ ++ +++ ++ +++ ++ − ++ 
 MRPm5 Mouse IgG2a M1V  986–1204 ++ + +++ ++ ++ − − − 
 MRPm6 Mouse IgG1 M1III 1511–1520 +++ + +++ ++ +++ − − − 
 MIB6 Mouse IgM M1Vc ? + + − ++ ++ − − ND 
MRP2 M2I-4 Mouse IgG1 M2I  215–310 +++ + ++ ND +++ − − − 
 M2II-12 Mouse IgG2a M2II  852–954 ++ − + ND ++ − − − 
 M2III-5 Mouse IgG2b M2III 1339–1541 ++ − ++ ND ++ − ++ + 
 M2III-6 Mouse IgG2a M2III 1339–1541 +++ + +++ + ++ − +++ ++ 
MRP3 M3II-9 Mouse IgG1 M3II  830–949 +++ − +++ + +++ − − − 
 M3II-21 Mouse IgG1 M3II  830–949 +++ − +++ ND +++ − − − 
MRP5 M5I-1 Rat IgG2a M5I   82–168 +++ − ++ + +++ ND ND ND 
 M5II-54 Rat IgG2a M5II  722–910 + − + ND + ND ND ND 
Transporter proteinMabAntigenReactivitya
NameSpeciesIsotypeFusion proteinAmino acid sequenceCytospins/Frozen sectionsParaffin sectionsWestern blotFACSSpecies
HumanMouseRatGuinea Pig
MDR1 P-gp JSB1 Mouse IgG1 NAb ? +++ + + ++ +++ − − − 
 C219 Mouse IgG2a NA 569–74+ 1214–19 +++ ++ +++ ++ +++ ++ ++ ++ 
MDR3 P-gp P3II-1 Mouse IgG1 P3II  629–692 ++ +/− + ND ++ − +/− +/− 
 P3II-26 Mouse IgG2b P3II  629–692 +++ +/− +++ ND +++ +/− + ++ 
MRP1 MRPr1 Rat IgG2a M1I  238–247 +++ ++ +++ ++ +++ ++ − ++ 
 MRPm5 Mouse IgG2a M1V  986–1204 ++ + +++ ++ ++ − − − 
 MRPm6 Mouse IgG1 M1III 1511–1520 +++ + +++ ++ +++ − − − 
 MIB6 Mouse IgM M1Vc ? + + − ++ ++ − − ND 
MRP2 M2I-4 Mouse IgG1 M2I  215–310 +++ + ++ ND +++ − − − 
 M2II-12 Mouse IgG2a M2II  852–954 ++ − + ND ++ − − − 
 M2III-5 Mouse IgG2b M2III 1339–1541 ++ − ++ ND ++ − ++ + 
 M2III-6 Mouse IgG2a M2III 1339–1541 +++ + +++ + ++ − +++ ++ 
MRP3 M3II-9 Mouse IgG1 M3II  830–949 +++ − +++ + +++ − − − 
 M3II-21 Mouse IgG1 M3II  830–949 +++ − +++ ND +++ − − − 
MRP5 M5I-1 Rat IgG2a M5I   82–168 +++ − ++ + +++ ND ND ND 
 M5II-54 Rat IgG2a M5II  722–910 + − + ND + ND ND ND 
a

−, no reactivity; +/−, very weak reactivity; +, weak reactivity; ++, good reactivity; +++, very good reactivity.

b

NA, not applicable; ?, unknown; ND,not determined.

c

For this immunization, booster injections with GLC4/ADR cells were also given.

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