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Table 2

Metabolism of NNK and MTBE by heterologously expressed human CYP2A6 and CYP2A13

Metabolism of NNK and MTBE was assayed as described in “Materials and Methods.” NNK (10 μm) and MTBE (0.25 mm)were incubated with the reconstituted CYPs at 37°C for 15 or 30 min,respectively. Where indicated, the molar ratio of CYP b5 was 1 4. The values reported are the average of two separate determinations with variations of<10% of the mean.

EnzymeRate of product formation (nmol/min/nmol CYP)
NNKMTBE
Keto aldehydeKeto alcoholtert-butyl alcohol
CYP2A13 1.8 0.45 1.1 
CYP2A13+ b5 2.2 0.62 1.4 
CYP2A6 0.024 n.d.a 1.1 
CYP2A6+ b5 0.036 n.d. 1.1 
EnzymeRate of product formation (nmol/min/nmol CYP)
NNKMTBE
Keto aldehydeKeto alcoholtert-butyl alcohol
CYP2A13 1.8 0.45 1.1 
CYP2A13+ b5 2.2 0.62 1.4 
CYP2A6 0.024 n.d.a 1.1 
CYP2A6+ b5 0.036 n.d. 1.1 
a

n.d., not detected.

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