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Table 1

Catalytic activities of CYP2A13 and CYP2A6 toward xenobiotic compounds

Reaction mixtures contained 50 mm phosphate buffer (pH 7.4), 1 mm NADPH, or an NADPH-generating system containing 0.4 mm NADP, 8.2 mm MgCl2, 10 mm glucose-6-phosphate, and 0.4 units/ml glucose-6-phosphate dehydrogenase, a reconstituted CYP system containing 0.1 μm recombinant CYP in Sf9 microsomes and 0.3 μm-purified rabbit NADPH-CYP reductase, and a substrate at the indicated concentration. Reactions were carried out at 37°C for 20–50 min. The values presented, which are initial linear rates, are the average of duplicate determinations with differences <15% of the mean.

Substrate (concentration)ReactionRate of product formation (nmol/min/nmol CYP)
CYP2A13CYP2A6
Coumarin (0.1 mm7-Hydroxylation 0.26 2.2 
2,6-Dichlorobenzonitrile (0.03 mmProtein adduct formation 0.04 0.03 
N-Nitrosodiethylamine (1 mmN-Deethylation 0.81 0.58 
Hexamethylphosphoramide (1 mmN-Demethylation 7.5 1.8 
N,N-Dimethylaniline (1 mmN-Demethylation 8.0 2.0 
2′-Methoxyacetophenone (1 mmO-Demethylation 7.2 1.6 
N-Nitrosomethylphenylamine (1 mmN-Demethylation 10.6 4.3 
Substrate (concentration)ReactionRate of product formation (nmol/min/nmol CYP)
CYP2A13CYP2A6
Coumarin (0.1 mm7-Hydroxylation 0.26 2.2 
2,6-Dichlorobenzonitrile (0.03 mmProtein adduct formation 0.04 0.03 
N-Nitrosodiethylamine (1 mmN-Deethylation 0.81 0.58 
Hexamethylphosphoramide (1 mmN-Demethylation 7.5 1.8 
N,N-Dimethylaniline (1 mmN-Demethylation 8.0 2.0 
2′-Methoxyacetophenone (1 mmO-Demethylation 7.2 1.6 
N-Nitrosomethylphenylamine (1 mmN-Demethylation 10.6 4.3 
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