American Association for Cancer Research

Table 1

MSI, hMLH1 mutations, expression, and methylation status in human colorectal cancer cell lines

Cell line	MSI	hMLH1 mutation	hMLH1 expression	Methylation in different regions (%)^a
Cell line	MSI	hMLH1 mutation	hMLH1 expression		A^b		B^c	C^d	D^e
SW1116	−	wild type^f	+	62	0	0	0
HCT8	−		+	57	0	0	0
Colo201	−	wild type^f	+	60	0	0	0
Colo320	−	wild type^f	+	55	0	0	0
RW2982	−		+	61	0	0	0
RW7213	−		+	57	0	0	0
H498	−		+	59	0	0	0
VACO411	−		+	64	0	0	0
VACO10P	−		+	60	0	0	0
CaCo2	−	wild type^f	+	67	0	0	0
SW1463	−		+	40	0	0	0
HRT18	−		+	54	0	0	0
HT29	−	wild type^f	+	35	37	0	0
SW620	−	wild type^f	+	24	10	0	0
LS123	−		+	36	24	0	0
LS174T	+	exon 4, codon 117, ACG to ATG, Thr to Met^g	+	45	11	0	0
HCT116	+	exon 9, codon 252, TCA to TAA, Ser to Stop^h^,i	+	62	0	0	0
C1a	+	exon 5, codon 129, AGT to GGT, Ser to Gly^g	−	95	82	100	89
SW48	+	wild type^f^,j	−	98	96	100	93
VACO5	+	wild type^f^,i^,j	−	98	96	100	100
VACO6	+	wild type^h^,i	−	95	80	100	38
RKO	+	wild typeⁱ	−	79	82	94	76
C	+	wild type^g	−	90	85	98	79
Lovo^k	+	wild type^f	+	60	0	0	0

Cell line	MSI	hMLH1 mutation	hMLH1 expression	Methylation in different regions (%)^a
Cell line	MSI	hMLH1 mutation	hMLH1 expression		A^b		B^c	C^d	D^e
SW1116	−	wild type^f	+	62	0	0	0
HCT8	−		+	57	0	0	0
Colo201	−	wild type^f	+	60	0	0	0
Colo320	−	wild type^f	+	55	0	0	0
RW2982	−		+	61	0	0	0
RW7213	−		+	57	0	0	0
H498	−		+	59	0	0	0
VACO411	−		+	64	0	0	0
VACO10P	−		+	60	0	0	0
CaCo2	−	wild type^f	+	67	0	0	0
SW1463	−		+	40	0	0	0
HRT18	−		+	54	0	0	0
HT29	−	wild type^f	+	35	37	0	0
SW620	−	wild type^f	+	24	10	0	0
LS123	−		+	36	24	0	0
LS174T	+	exon 4, codon 117, ACG to ATG, Thr to Met^g	+	45	11	0	0
HCT116	+	exon 9, codon 252, TCA to TAA, Ser to Stop^h^,i	+	62	0	0	0
C1a	+	exon 5, codon 129, AGT to GGT, Ser to Gly^g	−	95	82	100	89
SW48	+	wild type^f^,j	−	98	96	100	93
VACO5	+	wild type^f^,i^,j	−	98	96	100	100
VACO6	+	wild type^h^,i	−	95	80	100	38
RKO	+	wild typeⁱ	−	79	82	94	76
C	+	wild type^g	−	90	85	98	79
Lovo^k	+	wild type^f	+	60	0	0	0

The average of methylation percentage of all CpG sites in each region.

From −711 to −577, containing 23 CpG sites.

From −552 to −266, containing 19 CpG sites.

From −248 to −178, containing eight CpG sites.

From −109 to +15, containing seven CpG sites.

Ref. 17.

Determined in this study.

Ref. 6.

Ref. 14.

Ref. 8.

Lovo shows the expression of truncated RNA (exons 4–8 deletion) of hMSH2 gene.