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Table 1

Reduced mutation rate in hMLH1-expressing mouse Mlh1-deficient fibroblasts

Cell lineBase substitution rate ouabain resistance (× 10−7)a% microsatellite mutation dinucleotide (CA)nb
Wild typec ≤2 
mMlh1−/− + controld 60 10 
mMlh1−/− + hMLH1e  0.5 
 low expression ≤1  
 intermediate expression  
 high expression  
Cell lineBase substitution rate ouabain resistance (× 10−7)a% microsatellite mutation dinucleotide (CA)nb
Wild typec ≤2 
mMlh1−/− + controld 60 10 
mMlh1−/− + hMLH1e  0.5 
 low expression ≤1  
 intermediate expression  
 high expression  
a

The mutation rates (mutations/cell/generation) to ouabain resistance were calculated as described (6).

b

Dinucleotide changes were assayed with DNA from 8–10 clonal cultures. Frequencies are shown as a percentage of total changes/total number of alleles detectable at 5–8 loci.

c

Data from two wild type lines (6).

d

Data from two control-transfected cell lines were combined, CT-1 and CT-3 for ouabain resistance and CT-1 and CT-8 for microsatellite mutation.

e

For ouabain resistance, data from two cell lines each expressing hMLH1 at low (ME-2 and -6), intermediate (ME-9 and -10), or high (ME-4 and -5) relative level were combined. For microsatellite mutation, data from three cell lines (ME-6, -9, and -5) were combined.

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