Urinary estrogen metabolite ratiosa
| . | Baseline (n = 18) . | Control (n = 18) . | Low-Iso (n = 17)b . | High-Iso (n = 18) . |
|---|---|---|---|---|
| Genotoxic:Total | 0.16 ± 0.01c | 0.14 ± 0.01d | 0.12 ± 0.01d | 0.13 ± 0.01d |
| 2-(OH)E1:16α-(OH)E1e | 3.82 ± 0.33 | 4.54 ± 0.37 | 5.09 ± 0.38 | 5.13 ± 0.37 |
| 3.13 (2.51–3.90)c | 3.95 (3.38–4.62)c,d | 4.39 (3.73–5.16)d | 4.03 (3.45–4.72)c,d | |
| 2E1-total:16α-total | 1.33 ± 0.08 | 1.51 ± 0.07 | 1.56 ± 0.07 | 1.53 ± 0.07 |
| 2E1-total:4E1-total | 4.13 ± 0.12c | 5.16 ± 0.31d | 6.25 ± 0.33f | 5.90 ± 0.31d,f |
| 2E2-total:4E2-total | 3.29 ± 0.19c | 3.91 ± 0.30d | 3.72 ± 0.32c,d | 3.84 ± 0.30c,d |
| 2-total:4-total | 3.80 ± 0.11c | 4.67 ± 0.24d | 5.14 ± 0.25d | 5.05 ± 0.24d |
| . | Baseline (n = 18) . | Control (n = 18) . | Low-Iso (n = 17)b . | High-Iso (n = 18) . |
|---|---|---|---|---|
| Genotoxic:Total | 0.16 ± 0.01c | 0.14 ± 0.01d | 0.12 ± 0.01d | 0.13 ± 0.01d |
| 2-(OH)E1:16α-(OH)E1e | 3.82 ± 0.33 | 4.54 ± 0.37 | 5.09 ± 0.38 | 5.13 ± 0.37 |
| 3.13 (2.51–3.90)c | 3.95 (3.38–4.62)c,d | 4.39 (3.73–5.16)d | 4.03 (3.45–4.72)c,d | |
| 2E1-total:16α-total | 1.33 ± 0.08 | 1.51 ± 0.07 | 1.56 ± 0.07 | 1.53 ± 0.07 |
| 2E1-total:4E1-total | 4.13 ± 0.12c | 5.16 ± 0.31d | 6.25 ± 0.33f | 5.90 ± 0.31d,f |
| 2E2-total:4E2-total | 3.29 ± 0.19c | 3.91 ± 0.30d | 3.72 ± 0.32c,d | 3.84 ± 0.30c,d |
| 2-total:4-total | 3.80 ± 0.11c | 4.67 ± 0.24d | 5.14 ± 0.25d | 5.05 ± 0.24d |
Least squares mean ±SE. Within a row, means not sharing a superscript (c, d, or f) are significantly different (P <0.05). Genotoxic:Total, [16α-(OH)E1 +4-(OH)E2 + 4-(OH)E1]/total estrogens;2E1-total:16α-total, [2-(OH)E1 +2-MeOE1]:[16α-(OH)E1 +E3 + 17-epiE3];2E1-total:4E1-total,[2-(OH)E1 +2-MeOE1]:[4-(OH)E1 +4-MeOE1]; 2E2-total:4E2-total,[2-(OH)E2 +2-MeOE2]:4-(OH)E2; 2-total:4-total,[2-(OH)E1 + 2-(OH)E2 +2-MeOE1 +2-MeOE2]:[4-(OH)E1 +4-(OH)E2 + 4-MeOE1]. Only trace amounts of 4-MeOE2 were found, and data were not included in the calculation of ratios.
One subject did not collect urine samples at the end of low-iso diet period.
Because of unequal variance, data were log-transformed before ANOVA. Geometric means (95% confidence intervals) are presented below the least squares means.