Primer and probes specific for methylated DNA and used for MethyLight reactions
| HUGO gene nomenclature . | Forward primer sequence . | Reverse primer sequence . | Probe oligo sequence . |
|---|---|---|---|
| ACTB | TGGTGATGGAGGAGGTTTAGTAAGT | AACCAATAAAACCTACTCCTCCCTTAA | 6FAM-ACCACCACCCAACACACAATAACAAACACA-BHQ-1 |
| APC | GAACCAAAACGCTCCCCAT | TTATATGTCGGTTACGTGCGTTTATAT | 6FAM-CCCGTCGAAAACCCGCCGATTA-BHQ-1 |
| ARHI | GCGTAAGCGGAATTTATGTTTGT | CCGCGATTTTATATTCCGACTT | 6FAM-CGCACAAAAACGAAATACGAAAACGCAAA-BHQ-1 |
| BLT1 | GCGTTGGTTTTATCGGAAGG | AAACCGTAATTCCCGCTCG | 6FAM-GACTCCGCCCAACTTCGCCAAAA-BHQ-1 |
| BRCA1 | GAGAGGTTGTTGTTTAGCGGTAGTT | CGCGCAATCGCAATTTTAAT | 6FAM-CCGCGCTTTTCCGTTACCACGA-BHQ-1 |
| CALCA | GTTTTGGAAGTATGAGGGTGACG | TTCCCGCCGCTATAAATCG | 6FAM-ATTCCGCCAATACACAACAACCAATAAACG-BHQ-1 |
| CDH1 | AATTTTAGGTTAGAGGGTTATCGCGT | TCCCCAAAACGAAACTAACGAC | 6FAM-CGCCCACCCGACCTCGCAT-BHQ-1 |
| CDH13 | AATTTCGTTCGTTTTGTGCGT | CTACCCGTACCGAACGATCC | 6FAM-AACGCAAAACGCGCCCGACA-BHQ-1 |
| CDKN2A | TGGAGTTTTCGGTTGATTGGTT | AACAACGCCCGCACCTCCT | 6FAM-ACCCGACCCCGAACCGCG-BHQ-1 |
| CYP1B1 | GTGCGTTTGGACGGGAGTT | AACGCGACCTAACAAAACGAA | 6FAM-CGCCGCACACCAAACCGCTT-BHQ-1 |
| DAPK1 | TCGTCGTCGTTTCGGTTAGTT | TCCCTCCGAAACGCTATCG | 6FAM-CGACCATAAACGCCAACGCCG-BHQ-1 |
| ESR1 | GGCGTTCGTTTTGGGATTG | GCCGACACGCGAACTCTAA | 6FAM-CGATAAAACCGAACGACCCGACGA-BHQ-1 |
| ESR2 | TTTGAAATTTGTAGGGCGAAGAGTAG | ACCCGTCGCAACTCGAATAA | 6FAM-CCGACCCAACGCTCGCCG-BHQ-1 |
| FGF18 | ATCTCCTCCTCCGCGTCTCT | TCGCGCGTAGAAAACGTTT | 6FAM-CGACCGTACGCATCGCCGC-BHQ-1 |
| GSTM3 | GCGCGAACGCCCTAACT | AACGTCGGTATTAGTCGCGTTT | 6FAM-CCCCGTTCTCCGTCCCTTACCTCC-BHQ-1 |
| GSTP1 | GTCGGCGTCGTGATTTAGTATTG | AAACTACGACGACGAAACTCCAA | 6FAM-AAACCTCGCGACCTCCGAACCTTATAAAA-BHQ-1 |
| HIC1 | GTTAGGCGGTTAGGGCGTC | CCGAACGCCTCCATCGTAT | 6FAM-CAACATCGTCTACCCAACACACTCTCCTACG-BHQ-1 |
| HLA-G | CACCCCCATATACGCGCTAA | GGTCGTTACGTTTCGGGTAGTTTA | 6FAM-CGCGCTCACACGCTCAAAAACCT-BHQ-1 |
| HSD17B4 | TATCGTTGAGGTTCGACGGG | TCCAACCTTCGCATACTCACC | 6FAM-CCCGCGCCGATAACCAATACCA-BHQ-1 |
| HSPA2 | CACGAACACTACCAACAACTCAACT | GGGAGCGGATTGGGTTTG | 6FAM-CCGCGCCCAATTCCCGATTCT-BHQ-1 |
| IGFBP2 | CTCGCGCCGACAAATAAATAC | CGGGAAGAGTAGGGAATTTTTAGAGT | 6FAM-ACGCCCGCTCGCCCACCT-BHQ-1 |
| MGMT | GCGTTTCGACGTTCGTAGGT | CACTCTTCCGAAAACGAAACG | 6FAM-CGCAAACGATACGCACCGCGA-BHQ-1 |
| MLH1 | AGGAAGAGCGGATAGCGATTT | TCTTCGTCCCTCCCTAAAACG | 6FAM-CCCGCTACCTAAAAAAATATACGCTTACGCG-BHQ-1 |
| MLLT7 | CCTCACGATACCTCCCCTCAA | TTAGGGATTAGCGTTTTGGGATT | 6FAM-AAACACATTCCTACCAATCTTCAAAAAATCGCG-BHQ-1 |
| MT3 | CGATAAACGAACTTCTCCAAACAA | GCGCGGTGCGTAGGG | 6FAM-AAACGCGCGACTTAACTAATAACAACAAATAACGA-BHQ-1 |
| MYOD1 | GAGCGCGCGTAGTTAGCG | TCCGACACGCCCTTTCC | 6FAM-CTCCAACACCCGACTACTATATCCGCGAAA-BHQ-1 |
| PGR | TTATAATTCGAGGCGGTTAGTGTTT | TCGAACTTCTACTAACTCCGTACTACGA | 6FAM-ATCATCTCCGAAAATCTCAAATCCCAATAATACG-BHQ-1 |
| PPP1R13B | CCTCACCCACCGACATCATC | TCGGAGCGGTGGGTATAGTTC | 6FAM-AAAAATCCGCGACGCCCTCGA-BHQ-1 |
| PTGS2 | CGGAAGCGTTCGGGTAAAG | AATTCCACCGCCCCAAAC | 6FAM-TTTCCGCCAAATATCTTTTCTTCTTCGCA-BHQ-1 |
| RASSF1A | ATTGAGTTGCGGGAGTTGGT | ACACGCTCCAACCGAATACG | 6FAM-CCCTTCCCAACGCGCCCA-BHQ-1 |
| SOCS1 | GCGTCGAGTTCGTGGGTATTT | CCGAAACCATCTTCACGCTAA | 6FAM-ACAATTCCGCTAACGACTATCGCGCA-BHQ-1 |
| SOCS2 | TCCCTTCCCCGCCATT | TTGTTTTTGTCGCGGTGATTT | 6FAM-CCGAAAAACTCAAAACACCGCAAAATCAT-BHQ-1 |
| SYK | GGGCGCGATATTGGGAG | GCGACTCTTCCTCATTTTAAACAAC | 6FAM-CCTTAACGCGCCCGAACAAACG-BHQ-1 |
| TERT | GGATTCGCGGGTATAGACGTT | CGAAATCCGCGCGAAA | 6FAM-CCCAATCCCTCCGCCACGTAAAA-BHQ-1 |
| TFF1 | TAAGGTTACGGTGGTTATTTCGTGA | ACCTTAATCCAAATCCTACTCATATCTAAAA | 6FAM-CCCTCCCGCCAAAATAAATACTATACTCACTACAAAA-BHQ-1 |
| TIMP3 | GCGTCGGAGGTTAAGGTTGTT | CTCTCCAAAATTACCGTACGCG | 6FAM-AACTCGCTCGCCCGCCGAA-BHQ-1 |
| TITF1 | CGAAATAAACCGAATCCTCCTTAA | TGTTTTGTTGTTTTAGCGTTTACGT | 6FAM-CTCGCGTTTATTTTAACCCGACGCCA-BHQ-1 |
| TP53BP2 | ACCCCCTAACGCGACTTTATC | GTTCGATTCGGGATTAGTTGGT | 6FAM-CGCTCGTAACGATCGAAACTCCCTCCT-BHQ-1 |
| TWIST | GTAGCGCGGCGAACGT | AAACGCAACGAATCATAACCAAC | 6FAM-CCAACGCACCCAATCGCTAAACGA-BHQ-1 |
| HUGO gene nomenclature . | Forward primer sequence . | Reverse primer sequence . | Probe oligo sequence . |
|---|---|---|---|
| ACTB | TGGTGATGGAGGAGGTTTAGTAAGT | AACCAATAAAACCTACTCCTCCCTTAA | 6FAM-ACCACCACCCAACACACAATAACAAACACA-BHQ-1 |
| APC | GAACCAAAACGCTCCCCAT | TTATATGTCGGTTACGTGCGTTTATAT | 6FAM-CCCGTCGAAAACCCGCCGATTA-BHQ-1 |
| ARHI | GCGTAAGCGGAATTTATGTTTGT | CCGCGATTTTATATTCCGACTT | 6FAM-CGCACAAAAACGAAATACGAAAACGCAAA-BHQ-1 |
| BLT1 | GCGTTGGTTTTATCGGAAGG | AAACCGTAATTCCCGCTCG | 6FAM-GACTCCGCCCAACTTCGCCAAAA-BHQ-1 |
| BRCA1 | GAGAGGTTGTTGTTTAGCGGTAGTT | CGCGCAATCGCAATTTTAAT | 6FAM-CCGCGCTTTTCCGTTACCACGA-BHQ-1 |
| CALCA | GTTTTGGAAGTATGAGGGTGACG | TTCCCGCCGCTATAAATCG | 6FAM-ATTCCGCCAATACACAACAACCAATAAACG-BHQ-1 |
| CDH1 | AATTTTAGGTTAGAGGGTTATCGCGT | TCCCCAAAACGAAACTAACGAC | 6FAM-CGCCCACCCGACCTCGCAT-BHQ-1 |
| CDH13 | AATTTCGTTCGTTTTGTGCGT | CTACCCGTACCGAACGATCC | 6FAM-AACGCAAAACGCGCCCGACA-BHQ-1 |
| CDKN2A | TGGAGTTTTCGGTTGATTGGTT | AACAACGCCCGCACCTCCT | 6FAM-ACCCGACCCCGAACCGCG-BHQ-1 |
| CYP1B1 | GTGCGTTTGGACGGGAGTT | AACGCGACCTAACAAAACGAA | 6FAM-CGCCGCACACCAAACCGCTT-BHQ-1 |
| DAPK1 | TCGTCGTCGTTTCGGTTAGTT | TCCCTCCGAAACGCTATCG | 6FAM-CGACCATAAACGCCAACGCCG-BHQ-1 |
| ESR1 | GGCGTTCGTTTTGGGATTG | GCCGACACGCGAACTCTAA | 6FAM-CGATAAAACCGAACGACCCGACGA-BHQ-1 |
| ESR2 | TTTGAAATTTGTAGGGCGAAGAGTAG | ACCCGTCGCAACTCGAATAA | 6FAM-CCGACCCAACGCTCGCCG-BHQ-1 |
| FGF18 | ATCTCCTCCTCCGCGTCTCT | TCGCGCGTAGAAAACGTTT | 6FAM-CGACCGTACGCATCGCCGC-BHQ-1 |
| GSTM3 | GCGCGAACGCCCTAACT | AACGTCGGTATTAGTCGCGTTT | 6FAM-CCCCGTTCTCCGTCCCTTACCTCC-BHQ-1 |
| GSTP1 | GTCGGCGTCGTGATTTAGTATTG | AAACTACGACGACGAAACTCCAA | 6FAM-AAACCTCGCGACCTCCGAACCTTATAAAA-BHQ-1 |
| HIC1 | GTTAGGCGGTTAGGGCGTC | CCGAACGCCTCCATCGTAT | 6FAM-CAACATCGTCTACCCAACACACTCTCCTACG-BHQ-1 |
| HLA-G | CACCCCCATATACGCGCTAA | GGTCGTTACGTTTCGGGTAGTTTA | 6FAM-CGCGCTCACACGCTCAAAAACCT-BHQ-1 |
| HSD17B4 | TATCGTTGAGGTTCGACGGG | TCCAACCTTCGCATACTCACC | 6FAM-CCCGCGCCGATAACCAATACCA-BHQ-1 |
| HSPA2 | CACGAACACTACCAACAACTCAACT | GGGAGCGGATTGGGTTTG | 6FAM-CCGCGCCCAATTCCCGATTCT-BHQ-1 |
| IGFBP2 | CTCGCGCCGACAAATAAATAC | CGGGAAGAGTAGGGAATTTTTAGAGT | 6FAM-ACGCCCGCTCGCCCACCT-BHQ-1 |
| MGMT | GCGTTTCGACGTTCGTAGGT | CACTCTTCCGAAAACGAAACG | 6FAM-CGCAAACGATACGCACCGCGA-BHQ-1 |
| MLH1 | AGGAAGAGCGGATAGCGATTT | TCTTCGTCCCTCCCTAAAACG | 6FAM-CCCGCTACCTAAAAAAATATACGCTTACGCG-BHQ-1 |
| MLLT7 | CCTCACGATACCTCCCCTCAA | TTAGGGATTAGCGTTTTGGGATT | 6FAM-AAACACATTCCTACCAATCTTCAAAAAATCGCG-BHQ-1 |
| MT3 | CGATAAACGAACTTCTCCAAACAA | GCGCGGTGCGTAGGG | 6FAM-AAACGCGCGACTTAACTAATAACAACAAATAACGA-BHQ-1 |
| MYOD1 | GAGCGCGCGTAGTTAGCG | TCCGACACGCCCTTTCC | 6FAM-CTCCAACACCCGACTACTATATCCGCGAAA-BHQ-1 |
| PGR | TTATAATTCGAGGCGGTTAGTGTTT | TCGAACTTCTACTAACTCCGTACTACGA | 6FAM-ATCATCTCCGAAAATCTCAAATCCCAATAATACG-BHQ-1 |
| PPP1R13B | CCTCACCCACCGACATCATC | TCGGAGCGGTGGGTATAGTTC | 6FAM-AAAAATCCGCGACGCCCTCGA-BHQ-1 |
| PTGS2 | CGGAAGCGTTCGGGTAAAG | AATTCCACCGCCCCAAAC | 6FAM-TTTCCGCCAAATATCTTTTCTTCTTCGCA-BHQ-1 |
| RASSF1A | ATTGAGTTGCGGGAGTTGGT | ACACGCTCCAACCGAATACG | 6FAM-CCCTTCCCAACGCGCCCA-BHQ-1 |
| SOCS1 | GCGTCGAGTTCGTGGGTATTT | CCGAAACCATCTTCACGCTAA | 6FAM-ACAATTCCGCTAACGACTATCGCGCA-BHQ-1 |
| SOCS2 | TCCCTTCCCCGCCATT | TTGTTTTTGTCGCGGTGATTT | 6FAM-CCGAAAAACTCAAAACACCGCAAAATCAT-BHQ-1 |
| SYK | GGGCGCGATATTGGGAG | GCGACTCTTCCTCATTTTAAACAAC | 6FAM-CCTTAACGCGCCCGAACAAACG-BHQ-1 |
| TERT | GGATTCGCGGGTATAGACGTT | CGAAATCCGCGCGAAA | 6FAM-CCCAATCCCTCCGCCACGTAAAA-BHQ-1 |
| TFF1 | TAAGGTTACGGTGGTTATTTCGTGA | ACCTTAATCCAAATCCTACTCATATCTAAAA | 6FAM-CCCTCCCGCCAAAATAAATACTATACTCACTACAAAA-BHQ-1 |
| TIMP3 | GCGTCGGAGGTTAAGGTTGTT | CTCTCCAAAATTACCGTACGCG | 6FAM-AACTCGCTCGCCCGCCGAA-BHQ-1 |
| TITF1 | CGAAATAAACCGAATCCTCCTTAA | TGTTTTGTTGTTTTAGCGTTTACGT | 6FAM-CTCGCGTTTATTTTAACCCGACGCCA-BHQ-1 |
| TP53BP2 | ACCCCCTAACGCGACTTTATC | GTTCGATTCGGGATTAGTTGGT | 6FAM-CGCTCGTAACGATCGAAACTCCCTCCT-BHQ-1 |
| TWIST | GTAGCGCGGCGAACGT | AAACGCAACGAATCATAACCAAC | 6FAM-CCAACGCACCCAATCGCTAAACGA-BHQ-1 |