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Table 1

Specific cytotoxicity of SS dsFv and (dsFv)2 immunotoxins

Cytotoxicity assays were performed by measuring incorporation of [3H]Leu into cellular proteins as described in “Materials and Methods.” IC50 is the concentration that causes 50% inhibition of protein synthesis after 20 h incubation with immunotoxin.








Cell lineMeso-antigenaIC50, pmoles


dsFv(dsFv)2Fold increase
A431/K5 ++++ 18 25 
N87 ++ 180 18 10 
AGS ++ 90 23 
A1847 120 82  
A431
 

 
>7770
 
>7770
 

 
Cytotoxicity assays were performed by measuring incorporation of [3H]Leu into cellular proteins as described in “Materials and Methods.” IC50 is the concentration that causes 50% inhibition of protein synthesis after 20 h incubation with immunotoxin.








Cell lineMeso-antigenaIC50, pmoles


dsFv(dsFv)2Fold increase
A431/K5 ++++ 18 25 
N87 ++ 180 18 10 
AGS ++ 90 23 
A1847 120 82  
A431
 

 
>7770
 
>7770
 

 
a

Antigen density was measured by immunofluorescence using K1 antibody (5). ++++, high; ++, medium; +, low; −, no expression of mesothelin.

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