Expression of MMP-2 and MMP-9 mRNA in vitro after treatment with ABX-IL8 and control IgG
| . | Mean optical density ± SDa . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | MMP-9 . | . | MMP-2 . | . | |||
| . | Control IgG . | ABX-IL8 . | Control IgG . | ABX-IL8 . | |||
| 253 J B-V | 3.8 ± 0.77 | 2.5 ± 0.40b | 3.9 ± 0.46 | 2.9 ± 0.44b | |||
| 253 J S-IL8 | 4.2 ± 0.46 | 3.8 ± 0.46b | 4.4 ± 0.37 | 3.6 ± 0.39b | |||
| UMUC-3 | N/Ac | N/A | 3.7 ± 0.42 | 3.1 ± 0.38b | |||
| . | Mean optical density ± SDa . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | MMP-9 . | . | MMP-2 . | . | |||
| . | Control IgG . | ABX-IL8 . | Control IgG . | ABX-IL8 . | |||
| 253 J B-V | 3.8 ± 0.77 | 2.5 ± 0.40b | 3.9 ± 0.46 | 2.9 ± 0.44b | |||
| 253 J S-IL8 | 4.2 ± 0.46 | 3.8 ± 0.46b | 4.4 ± 0.37 | 3.6 ± 0.39b | |||
| UMUC-3 | N/Ac | N/A | 3.7 ± 0.42 | 3.1 ± 0.38b | |||
Colorimetric in situ hybridization was performed in triplicates, and the intensity of color reaction was quantified in 5–10 areas in at least five high power fields/slide. This is one representative experiment of two.
All Ps < 0.01, when compared with controls.
N/A, not available as MMP-9 expressed at very low levels in UMUC-3 cells.