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Table 1

Technical conditions of immunostaining

AntigenM/PaAntibody cloneSourceRetrievingDilution
P53 M DO7 Dako, Glostrup, Denmark MW 10 min. (pH 6) 1/20 
P16INK4 P P16-C20 Santa Cruz Biotechnology, Santa Cruz, CA No 1/200 
Cyclin D1 M DCS-6 Calbiochem, Cambridge, United Kingdom MW 10 min. (pH 6) 1/4000 kit tyramine CSA; Dako 
Cyclin E M 13 A3 Novocastra L., Newcastle, United Kingdom MW M. S. Unmasker (pH 8) 1/50 
Bcl2 M 124 Dako MW 10 min. (pH 6) 1/25 
Bax M N19 Santa Cruz Biotechnology MW 10 min. (pH 6) 1/200 
AntigenM/PaAntibody cloneSourceRetrievingDilution
P53 M DO7 Dako, Glostrup, Denmark MW 10 min. (pH 6) 1/20 
P16INK4 P P16-C20 Santa Cruz Biotechnology, Santa Cruz, CA No 1/200 
Cyclin D1 M DCS-6 Calbiochem, Cambridge, United Kingdom MW 10 min. (pH 6) 1/4000 kit tyramine CSA; Dako 
Cyclin E M 13 A3 Novocastra L., Newcastle, United Kingdom MW M. S. Unmasker (pH 8) 1/50 
Bcl2 M 124 Dako MW 10 min. (pH 6) 1/25 
Bax M N19 Santa Cruz Biotechnology MW 10 min. (pH 6) 1/200 
a

M, mouse monoclonal antibody; P, rabbit polyclonal antibody; MW, microwave heating.

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