Effects of AGP and CLI on intracellular concentrations and proliferative activity of leukemic blasts from patient 001
| . | Imatinib intracellular concentrations (ng/ml)a . | Proliferation ([3H]thymidine uptake)b . |
|---|---|---|
| Control | 0 | 45,926 ± 2,622 |
| Imatinib | 3,492 ± 417 | 1,581 ± 281 |
| Imatinib + AGP | 162 ± 49 | 43,879 ± 4,159 |
| Imatinib + AGP + CLI | 843 ± 151 | 3,160 ± 831 |
| AGP | 0 | 41,518 ± 4,740 |
| CLI | 0 | 48,861 ± 3,157 |
| . | Imatinib intracellular concentrations (ng/ml)a . | Proliferation ([3H]thymidine uptake)b . |
|---|---|---|
| Control | 0 | 45,926 ± 2,622 |
| Imatinib | 3,492 ± 417 | 1,581 ± 281 |
| Imatinib + AGP | 162 ± 49 | 43,879 ± 4,159 |
| Imatinib + AGP + CLI | 843 ± 151 | 3,160 ± 831 |
| AGP | 0 | 41,518 ± 4,740 |
| CLI | 0 | 48,861 ± 3,157 |
Blasts (1.5 × 108) were incubated with imatinib (3 μm) and/or AGP (1.5 mg/ml) and CLI (20 μm) for 1 h at 37°C. Subsequently cells were centrifuged at 400 × g for 10 min at 4°C; the supernatant was accurately discarded, and the pellet was frozen immediately. Imatinib concentrations were determined on the pellet by HPLC.
An aliquot of cells was seeded, before centrifugation, in 96-well plates and the proliferative activity assessed after 54 h of culture by [3H]thymidine uptake assay.