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Table 5

Effects of AGP and CLI on intracellular concentrations and proliferative activity of leukemic blasts from patient 001

Imatinib intracellular concentrations (ng/ml)aProliferation ([3H]thymidine uptake)b
Control 45,926 ± 2,622 
Imatinib 3,492 ± 417 1,581 ± 281 
Imatinib + AGP 162 ± 49 43,879 ± 4,159 
Imatinib + AGP + CLI 843 ± 151 3,160 ± 831 
AGP 41,518 ± 4,740 
CLI 48,861 ± 3,157 
Imatinib intracellular concentrations (ng/ml)aProliferation ([3H]thymidine uptake)b
Control 45,926 ± 2,622 
Imatinib 3,492 ± 417 1,581 ± 281 
Imatinib + AGP 162 ± 49 43,879 ± 4,159 
Imatinib + AGP + CLI 843 ± 151 3,160 ± 831 
AGP 41,518 ± 4,740 
CLI 48,861 ± 3,157 
a

Blasts (1.5 × 108) were incubated with imatinib (3 μm) and/or AGP (1.5 mg/ml) and CLI (20 μm) for 1 h at 37°C. Subsequently cells were centrifuged at 400 × g for 10 min at 4°C; the supernatant was accurately discarded, and the pellet was frozen immediately. Imatinib concentrations were determined on the pellet by HPLC.

b

An aliquot of cells was seeded, before centrifugation, in 96-well plates and the proliferative activity assessed after 54 h of culture by [3H]thymidine uptake assay.

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