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Table 4

In vivo mRNA expression level and microvessel density after the therapy with angiogenesis inhibitor TNP-470 for established human TCC 253J B-V cells growing orthotopically in athymic nude mice

TherapymRNA expression indexaMicrovessel densityb
bFGFVEGFIL-8MMP-9MMP-2E-cadherin(per 200 ×/field)
Nontreated CTRL 144 86 88 119 100 96 84 ± 12 
CTRL (ethanol in arabic gum/saline) daily 100 100 100 100 100 100 133 ± 26 
TNP-470 (15 mg/kg) daily 72c 100 100 69c 100 101 90 ± 18c 
TNP-470 (35 mg/kg) daily 70c 99 99 66c 99 103 88 ± 12c 
TNP-470 (105 mg/kg) daily 68c 99 96 60c 99 103 78 ± 8c 
TNP-470 (35 mg/kg) 3 times per week 96 103 101 93 99 101 101 ± 11 
TNP-470 (105 mg/kg) weekly 99 105 101 100 101 104 115 ± 13 
TherapymRNA expression indexaMicrovessel densityb
bFGFVEGFIL-8MMP-9MMP-2E-cadherin(per 200 ×/field)
Nontreated CTRL 144 86 88 119 100 96 84 ± 12 
CTRL (ethanol in arabic gum/saline) daily 100 100 100 100 100 100 133 ± 26 
TNP-470 (15 mg/kg) daily 72c 100 100 69c 100 101 90 ± 18c 
TNP-470 (35 mg/kg) daily 70c 99 99 66c 99 103 88 ± 12c 
TNP-470 (105 mg/kg) daily 68c 99 96 60c 99 103 78 ± 8c 
TNP-470 (35 mg/kg) 3 times per week 96 103 101 93 99 101 101 ± 11 
TNP-470 (105 mg/kg) weekly 99 105 101 100 101 104 115 ± 13 
a

The intensity of the cytoplasmic color reaction was quantitated by an image analyzer and compared with the maximal intensity of poly(dT) color reaction in each sample. The results were presented as a number of each cell line compared with CTRL defined as 100.

b

Microvessel density was expressed as an average number of five highest areas identified within a single 200 ×/field.

c

P < 0.005 against CTRL (Mann-Whitney statistical comparison).

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