Issues

A, Hierarchical clustering of network outputs (Euclidean distance, single linkage algorithm). The visualization represents results from the propagation of all our samples (39 GB, 15 AA, and 5 A) through the trained models. Color coding is according to the 3 molecular subtypes which best characterize the samples (blue, ANGIO; red, INTER; orange, LOWER). The only tumor where ANN subtyping does not agree with histopathology is GB154 (highlighted by a black box). Tumors are annotated with genomic information for a total of 7 loci known to be involved in astrocytic tumor genesis and/or progression. Grey boxes indicate homozygous deletion (CDKN2A/CDKN2B/p14ARF), amplification (CDK4, MDM2 and EGFR), or loss of one allele with mutation of the remaining allele (RB1, TP53 and PTEN). Test samples marked 'TEST'. B, Kaplan-Meier survival plot of our 59 astrocytic tumors as defined by our ANN grading results. Blue line, ANGIO; Red line, INTER; Orange line, LOWER. C, Kaplan-Meier survival plot of the 76 Phillips samples as defined by our ANNgrading results using our 59 gene classifiers. D, Kaplan-Meier survival plot of the 65 Freije samples. For details, see Petalidis et al. in this issue.