Issues

Subcellular colocalization of an anti-p97 antibody-drug conjugate with a lysosomal marker in melanoma cells. SK-MEL-5 cells were treated with the anti-p97 antibody-auristatin conjugate, L49-vcMMAF, directly labeled with Alexa Fluor 594 for 48 hours. L49-vcMMAF (red) and the lysosomal marker CD107a (green) colocalize (yellow) in melanoma cells that are effectively killed by L49-vcMMAF. Chromosomal DNA is stained with DAPI (blue). For antibody-drug conjugates targeting p97, both antigen level and trafficking to the lysosomes are important factors for achieving robust in vitro cytotoxicity against cancer cells. For details, see Smith et al. in this issue.