Issues

Src inhibition blocks angiogenesis in an in vivo model. Surgical gel foam sponges were soaked in conditioned medium from L3.6pl pancreatic tumor cells, implanted subcutaneously into C3H mice, harvested 14 days later, and frozen sections were examined for CD31 indicative of mouse vessels (brown staining). Robust infiltration of vessels is induced by angiogenic factors in the culture medium (top left), but this process is almost completely inhibited when medium from cells treated with the Src inhibitor AP23846 (top right) is used. rIl8 and rVEGF directly added to the gel foam also induce angiogenesis (bottom left). This angiogenesis is not blocked when a single dose of AP23846 is added directly to the gel foam (bottom right), demonstrating that residual inhibitor in the culture medium was not responsible for decreased angiogenesis. For details, see Summy et al. in this issue.