Issues

Nitazoxanide, an FDA-approved antiparasitic agent, causes cell death in spheroids (indicated by the loss of GFP fluorescence), while cells in monolayer culture continue proliferating, as can be seen in the middle panels. In contrast, a standard chemotherapeutic agent, oxaliplatin, has a cytostatic effect in monolayer cells, while spheroids remain largely unaffected (bottom panels). For comparison, untreated controls are shown in top panels. For details, see the article by Senkowski and colleagues on page 1504.