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1 September 2014
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To elucidate the potential mode of action of MAP-based cytolytic fusion proteins in living cells, colocalization assayswere performed using EGFR+ A549 cells transfected with a SNAP-tagged tubulin DNA construct. Expressed SNAP-tagged tubulin molecules were labeled with SNAP-Cell TMR-Star (green). EGF-MAPf151 was used as a representative for MAP-based CFPs. MAPf151 was detected using mouse–anti-human Tau and goat–anti-mouse Alexa Fluor 647 antibodies (red). DAPI was used to counterstain the nucleus (blue). Using confocal fluorescence microscopy and a tubulin polymerization assay, it could be shown that MAP colocalized with and stabilized microtubules. For more details, see article by Hristodorov and colleagues on page 2194.Close Modal - PDF Icon PDF LinkTable of Contents
ISSN 1535-7163
EISSN 1538-8514
Highlights
Review
Small Molecule Therapeutics
Selective Release of a Cyclopamine Glucuronide Prodrug toward Stem-like Cancer Cell Inhibition in Glioblastoma
Anaïs Balbous; Brigitte Renoux; Ulrich Cortes; Serge Milin; Karline Guilloteau; Thibaut Legigan; Pierre Rivet; Odile Boissonnade; Sébastien Martin; Caroline Tripiana; Michel Wager; René Jean Bensadoun; Sébastien Papot; Lucie Karayan-Tapon
Translational Exposure–Efficacy Modeling to Optimize the Dose and Schedule of Taxanes Combined with the Investigational Aurora A Kinase Inhibitor MLN8237 (Alisertib)
Jessica J. Huck; Mengkun Zhang; Jerome Mettetal; Arijit Chakravarty; Karthik Venkatakrishnan; Xiaofei Zhou; Rob Kleinfield; Marc L. Hyer; Karuppiah Kannan; Vaishali Shinde; Andy Dorner; Mark G. Manfredi; Wen Chyi Shyu; Jeffrey A. Ecsedy
Antiproliferative Effects of CDK4/6 Inhibition in CDK4-Amplified Human Liposarcoma In Vitro and In Vivo
Yi-Xiang Zhang; Ewa Sicinska; Jeffrey T. Czaplinski; Stephen P. Remillard; Samuel Moss; Yuchuan Wang; Christopher Brain; Alice Loo; Eric L. Snyder; George D. Demetri; Sunkyu Kim; Andrew L. Kung; Andrew J. Wagner
Large Molecule Therapeutics
EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein
Dmitrij Hristodorov; Manal Amoury; Radoslav Mladenov; Judith Niesen; Katharina Arens; Nina Berges; Lea Hein; Stefano Di Fiore; Anh-Tuan Pham; Michael Huhn; Wijnand Helfrich; Rainer Fischer; Theo Thepen; Stefan Barth
Cancer Biology and Signal Transduction
Companion Diagnostics and Cancer Biomarkers
Pharmacogenetic Predictors of Outcome in Patients with Stage II and III Colon Cancer Treated with Oxaliplatin and Fluoropyrimidine-Based Adjuvant Chemotherapy
Ana Custodio; Juan Moreno-Rubio; Jorge Aparicio; Javier Gallego-Plazas; Ricardo Yaya; Joan Maurel; Nuria Rodríguez-Salas; Emilio Burgos; David Ramos; Ana Calatrava; Encarna Andrada; Esther Díaz-López; Antonio Sánchez; Rosario Madero; Paloma Cejas; Jaime Feliu
Corrections
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