Abstract
KIOM210091 is an ethanol extract of seeds from one of Brassicaceae plant which has been widely used to relieve cough, prevent asthma, and reduce edema as traditional medicine in Korea and China. However, little is known about its chemical property, biological activity, and the molecular mechanism.
In our preliminary experiments, KIOM210091 showed potent cytotoxic effect on all of our 30 panels of different cancer cell lines originated from different tissue origins such as lung, kidney, intestine, prostate, uterine, and lymphocytes. It is previously unknown if KIOM210091 is effective against tumors when delivered as a dietary component, so we attempted to assess the anti-tumorigenic property of KIOM210091 in vivo xenograft tumor system.
Here our date showed that KIOM210091 attenuated tumor growth in H460-injected Balb/c athymic nude mice. A group of mice (n=6) was treated with 250mg/kg/d of KIOM210091 ad lib for 5 days/week for 3 week-period. This group of mice showed inhibited xenografted H460 cell tumor growth which was comparable to those groups treated with chemotherapeutic agents, rapamycin and 5-fluorouracil. However, low dose (50mg/kg/d) treatment had little or no effect on inhibition of tumor growth. Additionally, there was no adverse effect of KIOM210091 treatment during 3 week period administration.
Bioassy-guided fractionation revealed that the cytotoxic compound of KIOM210091 was found in ethyl acetate fraction and purification of single active compound is on the way. This fraction showed enhanced cytotoxic effects on tumor cells compared to the 80% ethanol extract. Our data also suggested that KIOM210091 as well as the active fraction had an effect on inhibiting the proliferative kinases and inducing apoptosis-related proteins.
Our data demonstrated the anti-tumorigenic effect of orally administrated KIOM210091, as well as the signaling pathway modulation. This study implicates this relatively safe, natural compound as a potential therapeutic and chemopreventive agent for lung cancer.
This research was supported by Korea Institute of Oriental Medicine Grant #K11062.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2011 Nov 12-16; San Francisco, CA. Philadelphia (PA): AACR; Mol Cancer Ther 2011;10(11 Suppl):Abstract nr B172.
