Abstract
Breast cancer resistance protein (BCRP) confers multidrug resistance to cancer cells against agents such as SN-38 (an active metabolite of irinotecan), mitoxantrone, and topotecan. Among 59 human tumor cell lines tested, 6 cell lines, A549, NCI-H460, KM-12, HT-29, OVCAR-5, and RPMI8226, showed high BCRP expression. BCRP cDNA was isolated from 11 cancer cell lines and three variant cDNAs [G34A substituting Met for Val-12 (V12M), C421A substituting Lys for Gln-141 (Q141K), and 944–949 deletion lacking Ala-315 and Thr-316 (Δ315-6)] were identified. G34A and C421A variants were polymorphisms, and 944–949 deletion was a splicing variant. C421A BCRP-transfected PA317 cells showed markedly decreased protein expression and low-level drug resistance compared with wild-type BCRP-transfected cells when transfectants expressed similar levels of BCRP mRNA. G34A or 944–949-deleted BCRP-transfected PA317 cells showed similar or somewhat lower protein expression and drug resistance compared with wild-type BCRP-transfected cells. Of 124 healthy Japanese volunteers, 67 were wild-type, 48 were heterozygous, and 9 were homozygous for the C421A allele. These results suggest that some people possess the C421A polymorphic BCRP gene and express low amounts of Q141K BCRP. In addition to that, C376T polymorphism in exon 4 substituting stop codon for Gln-126 was found in 3 of the 124 general Japanese population. This C376T polymorphism may also have high impact because active BCRP protein will not be expressed from the C376T allele. Therefore, people with C376T and/or C421A polymorphisms may express low amounts of BCRP, and this low BCRP expression might result in hypersensitivity of normal cells to such anticancer drugs as irinotecan and mitoxantrone.
Introduction
ABC
The abbreviations used are: ABC, ATP-binding cassette; BCRP, breast cancer resistance protein; NCI, National Cancer Institute; RT-PCR, reverse transcription-PCR.
Identification of single nucleotide polymorphisms has become important work because single nucleotide polymorphisms in various genes might not only be simple genomic markers but may also have certain significance in the expression and/or function of their product proteins. For example, C3435T polymorphism in exon 26 of the MDR1 gene was shown to be closely associated with low expression levels of P-glycoprotein and high plasma digoxin levels (10).
This study aimed to investigate whether variation of the first structure of the BCRP gene present in nondrug-treated cell lines and a normal population might influence the expression and function of the protein. First, we screened BCRP expression in a panel of 59 cancer cell lines in the anticancer drug screening program of the NCI (Bethesda, MD). Next, the whole coding sequence of BCRP cDNA was determined in 11 cell lines, 5 of which highly expressed BCRP protein. We identified three variant BCRP cDNAs, G34A, C421A, and 944–949 deletion, and investigated functional outcomes. Incidences of G34A and C421A polymorphisms were examined in healthy Japanese volunteers. We report that C421A polymorphism is very frequent in the general Japanese population and may be associated with decreased protein expression and low-level drug resistance.
Materials and Methods
Cell Lines and Blood Samples.
Murine fibroblast PA317 cells were grown in Dulbecco’s modified essential medium supplemented with 10% fetal bovine serum at 37°C with 5% CO2. Peripheral blood nucleated cells were obtained from healthy Japanese volunteers after obtaining written informed consent for genetic analysis.
Western Blot Analysis.
Frozen cell pellets of 59 cell lines in the NCI anticancer drug screening were obtained from the NCI (11). Cell pellets for MDA-N in the 60 cell line panel were not available. Western blot analysis of BCRP was performed as described previously (12). Briefly, cell pellets were solubilized in a lysis buffer [10 mm Tris-HCl (pH 8.0), 0.1% Triton X-100, 10 mm MgSO4, 2 mm CaCl2, 1 mm 4-(2-aminoethyl)-benzenesulfonylfluoride with or without 1 mm DTT]. Cell lysate was solubilized with 2% SDS, 50 mm Tris-HCl (pH 7.5), in the presence or absence of 5% 2-mercaptoethanol, and resolved by SDS-PAGE. After electrophoresis, proteins were transferred onto nitrocellulose membranes and incubated with an anti-BCRP polyclonal antibody (12). Then, the blots were incubated with a peroxidase-conjugated donkey antirabbit secondary antibody (Amersham Pharmacia Biotech, Buckinghamshire, United Kingdom), and membrane-bound peroxidase was visualized using ECL Plus chemiluminescence detection kit (Amersham).
Sequence Analysis of the BCRP Gene.
The entire coding region of BCRP cDNA was generated by RT-PCR from total RNA by using RNA LA PCR kit (Takara, Kyoto, Japan) according to the manufacturer’s instructions. The primer set used was 5′-CGGATCCTCCTGAGATCCTGAGCCTTTGGTT-3′ and 5′-CGCTCTAGAGATGGCAAGGGAACAGAAAACAACA-3′. PCR products were inserted into pCR2.1 TA cloning vector (Invitrogen, Carlsbad, CA), and three to eight clones were sequenced with ABI Prism377 automatic sequencer (Applied Biosystems, Foster City, CA). Mutations observed in more than two clones were subjected to analysis of other clones derived from an independent RT-PCR product to exclude PCR-induced mutation.
Next, the exon-intron structure of the BCRP gene was determined by aligning the complete ABCP cDNA coding sequence (GenBank accession no. AF103796) and Homo sapiens BAC clone RP11-368G2 from 4 (GenBank accession no. AC084732). The genomic structure of the BCRP gene that we determined was the same as reported in the literature (13). Exon 2 that covers the 34th nucleotide of BCRP cDNA was amplified by PCR with the primer set 5′-GCAATCTCATTTATCTGGACTA-3′ and 5′-TGTGAGGTTCACTGTAGGTAAA-3′. Exon 5 that covers the 421st nucleotide of BCRP cDNA was amplified by PCR with the primer set 5′-CCTTAGTTATGTTATCTTTGTG-3′ and 5′-GAAACTTCTGAATCAGAGTCAT-3′. Exon 9 that includes the 944–949th nucleotides at the 5′-end was amplified with the primer set 5′-TTAGGGAAGCATCCAAGAAAG-3′ and 5′-GAAGCAGATGATAACAGAACC-3′. PCR products were either sequenced after TA subcloning or directly sequenced as described.
Establishment of Mutant BCRP-expressing Cells.
G34A, C421A, and 944–949-deleted BCRP cDNAs without any other mutations were inserted into a pHaL-IRES-DHFR bicistronic retrovirus vector plasmid. PA317 cells were transfected with those vectors by using a Mammalian Transfection kit (Stratagene, La Jolla, CA) and selected by exposure to 120 ng/ml methotrexate. Hundreds of drug-resistant colonies were pooled and used as BCRP-transfected cells. PA317 cells transfected with wild-type, G34A, C421A, and 944–949-deleted BCRP cDNAs were designated PA/WT, PA/V12M, PA/Q141K, and PA/Δ315-6 cells, respectively.
Northern Blot Analysis.
Twenty μg of total RNA was fractionated on a 1% agarose-formaldehyde gel and transferred to Hybond-N+ (Amersham). The blot was hybridized with a 456-bp fragment from 574th to 1029th nucleotides of BCRP cDNA by using AlkPhos Direct Labeling kit according to the manufacturer’s instructions (Amersham). Chemiluminescence signal generation and detection were performed with CDP-Star detection reagent according to the manufacturer’s instructions (Amersham).
Growth Inhibition Assay.
The sensitivity of cells to SN-38, mitoxantrone, and topotecan was evaluated by measuring cell growth inhibition after incubation of cells at 37°C for 5 days in the absence or presence of various concentrations of anticancer agents. Cell numbers were determined with a Coulter counter. IC50s (drug dose causing 50% inhibition of cell growth) were determined from growth inhibition curves, and the degrees of resistance were calculated by dividing IC50s of BCRP-transfected cells by those of parental PA317 cells.
Measurement of Intracellular Topotecan Uptake.
The effect of mutant BCRP on cellular accumulation of topotecan was determined by flow cytometry. Cells (5 × 105) were incubated with 30 μm topotecan for 30 min at 37°C, washed in ice-cold PBS, and subjected to fluorescence analysis using FACSCalibur (Becton-Dickinson, San Jose, CA).
Results
Western Blot Analysis of Tumor Cell Lines.
BCRP expression in 59 human tumor cell lines was examined by Western blotting in the absence of reducing agents. Under this condition, BCRP migrated as a homodimer and was detected as a Mr 140,000 band (12). Of the 59 cell lines analyzed, 6 cell lines (lung cancer, A549 and NCI-H460; colon cancer, HT-29 and KM-12; ovarian cancer, OVCAR-5; and leukemia, RPMI8226) showed considerably high BCRP expression (Fig. 1). The other 53 cell lines showed marginal or no BCRP expression. Western blotting of representative 6 cell lines with low or no BCRP expression was also shown in Fig. 1.
Isolation of BCRP cDNA Variants.
To identify BCRP cDNA variants and to examine the possible effect of the mutations on BCRP expression and function, BCRP cDNA clones were isolated from 11 human tumor cell lines, breast cancer MCF-7 and MDA-MB-231, lung cancer A549, NCI-H23, and NCI-H460, colon cancer HCT-116, HT-29, and KM-12, and ovarian cancer OVCAR-5, OVCAR-8, and SK-OV-3. BCRP cDNA could not be obtained from NCI-H226 cells that did not express BCRP protein. mRNA expression in MCF-7, MDA-MB-231, NCI-H23, HCT-116, OVCAR-8, and SK-OV-3 cells was very low as determined by RT-PCR, in accordance with very low or undetectable BCRP protein expression by Western blot analysis (Fig. 1). In cDNA sequence analysis, C421A mutation that substitutes Lys for Gln-141 was found in MDA-MB-231, A549, and HCT-116 cells (Table 1; Fig. 2). G34A mutation that substitutes Met for Val-12 was found in MCF-7 cells. Deletion of 944–949 resulting in the loss of Ala-316 and Thr-316 was noted in MCF-7, A549, HT-29, and SK-OV-3 cells (Table 1).
Next, the above-mentioned mutations/polymorphisms were confirmed by genomic DNA analysis. Aligning ABCP cDNA sequence and Homo sapiens BAC clone RP11-368G2 from 4 demonstrated that the 34th and 421st nucleotides were in exons 2 and 5, respectively. Nucleotides 944–949 were at the 5′-end of exon 9, after the splicing acceptor site. By genomic DNA analysis, G34A polymorphism in exon 2 was observed in MCF-7 cells. C421A polymorphism in exon 5 was noted in MDA-MB-231, A549, and HCT-116 cells (Fig. 2C). Deletion of nucleotides 944–949 was not observed with genomic analysis, suggesting that the 944–949-deleted mRNA is a splicing variant.
Western and Northern Blot Analyses of Mutant BCRP-expressing Cells.
Western blotting of mutant BCRP-transfected PA317 cells demonstrated markedly low expression of Q141K BCRP in PA/Q141K cells compared with other BCRP-transfected cells. PA/WT, PA/V12M, and PA/Δ315-6 cells showed similar BCRP expression (Fig. 3A). The results were confirmed by a second, independent transfection experiment of PA317 cells. BCRP expression levels in the transfected cell lines were stable for 4 months without any drug selection. In contrast, Northern blotting demonstrated similar levels of BCRP mRNA in PA/WT, PA/V12M, PA/Q141K, and PA/Δ315-6 cells (Fig. 3B). These results suggest that Q141K BCRP is unstable when expressed in mammalian cells.
Growth Inhibition Assay of Mutant BCRP-expressing Cells.
PA/WT cells showed 40-fold higher resistance to SN-38 and 10-fold higher resistance to mitoxantrone compared with parental PA317 cells (Table 2; Fig. 4). PA/WT cells also showed a greater than 10-fold resistance to topotecan (Table 2; Fig. 4). PA/WT and PA/V12M cells showed similar levels of resistance to the anticancer drugs (Table 2; Fig. 4A). PA/Δ315–6 cells were marginally more sensitive to the drugs than PA/WT cells (Table 2; Fig. 4B). In contrast, PA/Q141K cells showed a 12-fold greater resistance to SN-38 and a 4-fold greater resistance to mitoxantrone (Table 2; Fig. 4A). This means that PA/Q141K cells are 2–3 times more sensitive to these drugs compared with PA/WT cells. These results support the low expression of BCRP in PA/Q141K cells. Cross-resistance patterns of the transfectants were similar, suggesting that these polymorphisms/deletions did not affect substrate recognition of BCRP.
Measurement of Intracellular Topotecan Uptake.
When PA317 cells were incubated with topotecan, a fluorescence peak shifted to the right in parental PA317 cells, indicating cellular uptake of topotecan (Fig. 5). The mean fluorescence channel number of PA317 cells increased 4.6-fold in the presence of topotecan. In contrast, only a marginal shift occurred in PA/WT, PA/V12M, and PA/Δ315-6 cells (Fig. 5). Increases of mean fluorescence channel number in PA/WT, PA/V12M, and PA/Δ315-6 cells were 1.5-, 1.6-, and 1.5-fold in the presence of topotecan, respectively. There was a stronger peak shift to the right in PA/Q141K cells than PA/WT cells, showing that topotecan uptake in PA/Q141K cells was higher than that in PA/WT cells, and the increase of mean fluorescence channel number in PA/Q141K cells was 2.1-fold in the presence of topotecan (Fig. 5). This suggests that the topotecan efflux activity of exogenous BCRP is low in PA/Q141K cells.
Frequencies of G34A and C421A Polymorphisms.
Frequencies of G34A and C421A polymorphisms in the BCRP gene were examined in a normal (noncancer) Japanese population. Twenty-nine samples were used in the first screening, and because of the possible importance of C421A polymorphism, an additional 95 samples were used only for the allele. The results are summarized in Table 3. Of 124 samples examined, 67 were wild-type, 48 were heterozygous, and 9 were homozygous for the C421A allele. C421A polymorphism exhibited high frequency in this normal Japanese population.
In our subsequent genomic DNA analysis, C376T polymorphism in exon 4 substituting stop codon for Gln-126 was found in 3 (2.4%) of the 124 general Japanese population (Table 3). The 3 were all heterozygous for the C376T allele. This C376T polymorphism may have higher impact than C421A polymorphism because active BCRP protein will not be expressed from the C376T allele.
Discussion
In this study, we first examined BCRP expression by Western blotting in a panel of 59 cell lines in the NCI anticancer drug screening. Among these 59 cell lines, considerably high expression of BCRP was noted in 6 cell lines (10%), consisting of lung cancer, colon cancer, ovarian cancer, and leukemia cell lines, showing that BCRP was highly expressed in a variety of cancer cells without drug selection. BCRP is physiologically expressed in the placenta, digestive tract, genitalia, and hematopoietic stem cells (5, 14). Our result suggests that BCRP expression in cancer cells occurs irrespective of their primary origins and may be responsible for natural drug resistance of cancer cells.
In this study, we identified three BCRP cDNA variants. G34A and C421A are polymorphisms because they were observed with high frequencies in the general Japanese population. Nucleotides 944–949 followed the splicing acceptor site of exon 9, and genomic analysis did not reveal such deletion mutations. Therefore, the deletion of nucleotides 944–949 was considered to be a splicing variant between exons 8 and 9. This splicing variant mRNA is also expressed in normal individuals because the deleted cDNA has been also isolated from commercially available human placental cDNA (Marathone-ready cDNA; Clontech, data not shown).
PA/Q141K cells were significantly more sensitive to anticancer agents than the other BCRP transfectants. Intracellular topotecan accumulation of PA/Q141K was higher than that in the other BCRP transfectants. By Western blotting, BCRP expression in PA/Q141K cells was markedly lower than that in the other BCRP transfectants. Another transfection experiment of mutant BCRP cDNAs in KB-3-1 human epidermoid carcinoma cells also revealed markedly lower expression of Q141K BCRP compared with wild-type and V12M BCRP (data not shown). Although the 141st amino acid of BCRP is located in the functionally important ATP-binding region between Walker A and B, increased sensitivity to anticancer drugs was not because of functional alteration but because of decreased protein expression. In the transfection experiment, the expression of C421A BCRP mRNA was identical to those of mRNAs from wild-type, G34A, and 944–949-deleted BCRP by Northern blotting. Therefore, the increased sensitivity was considered to be a result of instability of Q141K BCRP. Because lysine and glutamine have different electronic charges, substitution of lysine for glutamine might alter the tertiary structure of BCRP protein, leading to greater susceptibility to degradation.
We first intended to correlate mutant cDNA with protein expression levels of cancer cell lines, but three variants were observed in either cell lines that highly expressed BCRP or those that did not express BCRP. For instance, BCRP was highly expressed in A549 cells that carry both the C421A and wild-type BCRP alleles. Protein expression levels can be influenced not only by mRNA sequences but also by genomic structures such as chromatin alterations, methylation, or acetylation. Increased BCRP expression in A549 cells could be explained by the high transcription rate of the wild-type BCRP gene.
In this study, we showed that 46% of a normal Japanese population carries the C421A allele and, in particular, 7% were homozygous. In the analysis of 59 tumor cell lines, 5 (8%) were heterozygous and 2 (3%) were homozygous for the C421A allele. Because most of these cell lines were established in Western countries, people with the C421A allele should exist at high frequency in Western countries as well. BCRP transports anticancer agents such as SN-38 and mitoxantrone. Irinotecan, a prodrug of SN-38, and mitoxantrone are used in practical chemotherapy for a wide variety of cancers. BCRP expressed in normal tissues of cancer patients may serve to reduce adverse effects of these drugs such as hematological toxicity and digestive tract disorders. Irinotecan in clinically administered dosages causes grade 3–4 leukopenia in ∼30% and grade 3–4 diarrhea in 20% of cancer patients according to the World Health Organization criteria (15, 16). These severe toxicities are presently unpredictable. Because BCRP is expressed in both hematopoietic stem cells and enterocytes of the digestive tract and may protect those cells against toxic compounds, administration of the drugs to patients with the C421A allele may cause severe side effects. We started additional studies to investigate the association of C421A polymorphism with side effects from irinotecan chemotherapy.
In our subsequent genomic DNA analysis, C376T polymorphism in exon 4 substituting stop codon for Gln-126 was found in 3 (2.4%) of the 124 general Japanese population. The 3 were all heterozygous for the C376T allele. This C376T polymorphism may have higher impact than C421A polymorphism because active BCRP protein will not be expressed from the C376T allele. Additional investigation is planned concerning mRNA expression of this C376T allele and possible implication of the C376T polymorphism in side effect of irinotecan.
In summary, C421A BCRP cDNA was associated with low protein expression and subsequent sensitivity to anticancer drugs compared with wild type. C421A polymorphism showed high frequency in the general Japanese population. Screening for C421A polymorphism in cancer patients before chemotherapy should be useful for the prevention of serious side effects of some anticancer drugs.
Variant . | Amino acid change . | Cell line . |
---|---|---|
G34A | Val-12 to Met | MCF-7a |
C421A | Gln-141 to Lys | MDA-MB-231a |
A549a | ||
HCT-116a | ||
Deletion of 944–949 | Deletion of Ala-315 and Thr-316 | MCF-7 |
A549 | ||
HT-29 | ||
SK-OV-3 |
Variant . | Amino acid change . | Cell line . |
---|---|---|
G34A | Val-12 to Met | MCF-7a |
C421A | Gln-141 to Lys | MDA-MB-231a |
A549a | ||
HCT-116a | ||
Deletion of 944–949 | Deletion of Ala-315 and Thr-316 | MCF-7 |
A549 | ||
HT-29 | ||
SK-OV-3 |
Heterozygous allele.
. | PA317 . | PA/WT . | PA/V12M . | PA/Q141K . | PA/Δ315-6 . |
---|---|---|---|---|---|
SN-38 | 2.5 | 98 | 98 | 30 | 55 |
Mitoxantrone | 0.060 | 0.58 | 0.63 | 0.25 | 0.42 |
Topotecan | 17 | >200 | >200 | 100 | 190 |
. | PA317 . | PA/WT . | PA/V12M . | PA/Q141K . | PA/Δ315-6 . |
---|---|---|---|---|---|
SN-38 | 2.5 | 98 | 98 | 30 | 55 |
Mitoxantrone | 0.060 | 0.58 | 0.63 | 0.25 | 0.42 |
Topotecan | 17 | >200 | >200 | 100 | 190 |
IC50s (drug dose causing 50% inhibition of cell growth) were determined from cell growth curves in each experiment.
. | Wild-type . | Heterozygous . | Homozygous . | Total . |
---|---|---|---|---|
G34A | 19 (66%) | 9 (31%) | 1 (3%) | 29 |
C376Ta | 121 (98%) | 3 (2%) | 0 (0%) | 124 |
C421A | 67 (54%) | 48 (39%) | 9 (7%) | 124 |
. | Wild-type . | Heterozygous . | Homozygous . | Total . |
---|---|---|---|---|
G34A | 19 (66%) | 9 (31%) | 1 (3%) | 29 |
C376Ta | 121 (98%) | 3 (2%) | 0 (0%) | 124 |
C421A | 67 (54%) | 48 (39%) | 9 (7%) | 124 |
C376T polymorphism in exon 4 substitutes stop codon for Gln-126.
References
Supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology, the Ministry of Health, Labour and Welfare, Japan, and the Virtual Research Institute of Aging of Nippon Boehringer Ingelheim.