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trpv6

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Journal Articles
Mol Cancer Res (2019) 17 (11): 2145–2153.
Published: 01 November 2019
...' sensitivity to chemotherapeutics in colorectal cancer. TRPV6 with high Ca2+ selectivity and constitutive activity is also overly expressed in human colon cells as compared with normal colonic epithelial cells by transcriptomic analysis of calcium remodeling in colorectal cancer ( 24...
Images
Specific examples of calcium channels, pumps, and receptors causing calcium...
Published: 01 November 2019
Figure 1. Specific examples of calcium channels, pumps, and receptors causing calcium changing in colorectal cancer cells. Plasmalemmal Ca2+ permeable ion channels are vital for regulating Ca2+ influx (e.g., the stimulation of TRPC5 and TRPV6 or the depletion of intracellular Ca2+ stores -ORAI1). Ca2+ pumps include plasma membrane calcium efflux pumps. For instance, PMCA4 controls the external flow of cells. SERCA refers to the endoplasmic reticulum Ca2+ sequestration pumps. ER stress and MCU act as the major repositories of Ca2+ in cell. ER is considered the primary regulator of cell death, while the role of mitochondria in apoptosis has been increasingly obvious. CaSR refers to a member of G Protein–coupled receptors. Figure 1. Specific examples of calcium channels, pumps, and receptors causing calcium changing in colorectal cancer cells. Plasmalemmal Ca2+ permeable ion channels are vital for regulating Ca2+ influx (e.g., the stimulation of TRPC5 and TRPV6 or the depletion of intracellular Ca2+ stores -ORAI1). Ca2+ pumps include plasma membrane calcium efflux pumps. For instance, PMCA4 controls the external flow of cells. SERCA refers to the endoplasmic reticulum Ca2+ sequestration pumps. ER stress and MCU act as the major repositories of Ca2+ in cell. ER is considered the primary regulator of cell death, while the role of mitochondria in apoptosis has been increasingly obvious. CaSR refers to a member of G Protein–coupled receptors. More
Journal Articles
Mol Cancer Res (2009) 7 (12): 2000–2010.
Published: 01 December 2009
...Katrin A. Bolanz; Gergely Gy. Kovacs; Christopher P. Landowski; Matthias A. Hediger The epithelial calcium channel TRPV6 is upregulated in breast carcinoma compared with normal mammary gland tissue. The selective estrogen receptor modulator tamoxifen is widely used in breast cancer therapy...
Images
Phase contrast image ( A ), EYFP image ( B ), and Fura-2 ratio image ( C ) ...
Published: 01 December 2009
FIGURE 5. Phase contrast image ( A ), EYFP image ( B ), and Fura-2 ratio image ( C ) of EYFP-C1-TRPV6–expressing MCF-7 cells. Note that TRPV6-expressing cells have higher basal intracellular calcium level. FIGURE 5. Phase contrast image (A), EYFP image (B), and Fura-2 ratio image (C) of EYFP-C1-... More
Images
A.  Comparison of <span class="search-highlight">TRPV6</span> mRNA expression (fold difference) in MCF-7, MDA-MB-...
Published: 01 December 2009
FIGURE 1. A. Comparison of TRPV6 mRNA expression (fold difference) in MCF-7, MDA-MB-231 cells, and T47D human breast cancer cells detected with real-time PCR. B. Comparison of TRPV6 protein in T47D, MDA-MB-231, and MCF-7 cells measured with immunopreciptiation and Western blotting. C. Phase contrast images (left) and immunofluorescence staining (right) of TRPV6 in MDA-MB-231 cells (a and b), MCF-7 (c and d), and T47D cells (e and f). Images were taken with a ×20 objective (scale bar, 20 μm). FIGURE 1. A. Comparison of TRPV6 mRNA expression (fold difference) in MCF-7, MDA-MB-231 cells, and T47D human breast cancer cells detected with real-time PCR. B. Comparison of TRPV6 protein in T47D, MDA-MB-231, and MCF-7 cells measured with immunopreciptiation and Western blotting. C. Phase contrast images (left) and immunofluorescence staining (right) of TRPV6 in MDA-MB-231 cells (a and b), MCF-7 (c and d), and T47D cells (e and f). Images were taken with a ×20 objective (scale bar, 20 μm). More
Images
Immunofluorescence double staining with anti-<span class="search-highlight">TRPV6</span> ( A ) and anti-PMCA anti...
Published: 01 December 2009
FIGURE 2. Immunofluorescence double staining with anti-TRPV6 ( A ) and anti-PMCA antibodies ( B ) in T47D cells. Arrows, the colocalization of TRPV6 and PMCA in the merged ( C ) image. D. The overlay of the phase contrast and the merged image. Images were taken with a ×20 objective (scale bar, 20 μm). FIGURE 2. Immunofluorescence double staining with anti-TRPV6 (A) and anti-PMCA antibodies (B) in T47D cells. Arrows, the colocalization of TRPV6 and PMCA in the merged (C) image. D. The overlay of the phase contrast and the merged image. Images were taken with a ×20 objective (scale bar, 20 μm). More
Images
Phase contrast ( A ) and fluorescence EYFP image ( B ) to display localizat...
Published: 01 December 2009
FIGURE 4. Phase contrast ( A ) and fluorescence EYFP image ( B ) to display localization of EYFP-C1-TRPV6 after transient transfection in MCF-7 cells. Images were taken with a ×60 objective (scale bar, 20 μm). Arrows, plasma membrane localization. FIGURE 4. Phase contrast (A) and fluorescence EYFP image (B) to display localization of EYFP-C1-TRPV6 after transient transfection in MCF-7 cells. Images were taken with a ×60 objective (scale bar, 20 μm). Arrows, plasma membrane localization. More
Images
Representative tracings showing the effect of 10 μmol/L tamoxifen citrate o...
Published: 01 December 2009
FIGURE 7. Representative tracings showing the effect of 10 μmol/L tamoxifen citrate on intracellular [Ca2+] ([Ca2+]i) in EYFP-C1-TRPV6–expressing MCF-7 cells in the presence of 1 mmol/L extracellular Ca2+ ( A ) or in nominally calcium-free solution ( B ). FIGURE 7. Representative tracings showing the effect of 10 μmol/L tamoxifen citrate on intracellular [Ca2+] ([Ca2+]i) in EYFP-C1-TRPV6–expressing MCF-7 cells in the presence of 1 mmol/L extracellular Ca2+ (A) or in nominally calcium-free solution (B). More
Images
A.  Effect of pretreatment of transfected MCF-7 cells with the ER antagonis...
Published: 01 December 2009
FIGURE 9. A. Effect of pretreatment of transfected MCF-7 cells with the ER antagonist ICI 182.780 (1 μmol/L) for 10 min on TRPV6 activity with and without tamoxifen. Data are the means of at least 25 cells from six separate experiments. B. Effect of 10 μmol/L tamoxifen on calcium influx in ER-negative MDA-MB-231 cells. *, P < 0.05 control versus tamoxifen group. FIGURE 9. A. Effect of pretreatment of transfected MCF-7 cells with the ER antagonist ICI 182.780 (1 μmol/L) for 10 min on TRPV6 activity with and without tamoxifen. Data are the means of at least 25 cells from six separate experiments. B. Effect of 10 μmol/L tamoxifen on calcium influx in ER-negative MDA-MB-231 cells. *, P < 0.05 control versus tamoxifen group. More
Images
Summary data showing the rate of changes in the fura-2 ratio after readditi...
Published: 01 December 2009
FIGURE 8. Summary data showing the rate of changes in the fura-2 ratio after readdition of calcium in control group and in response to treatment with 1 μmol/L tamoxifen (Tam), 10 μmol/L tamoxifen, and 10 μmol/L 4-hydroxy-tamoxifen (4-H-Tam) treatment for 10 min and with 1 and 10 μmol/L tamoxifen treatment for 1 h in MCF-7 cells transfected with TRPV6. *, P < 0.05 treatment versus control group. FIGURE 8. Summary data showing the rate of changes in the fura-2 ratio after readdition of calcium in control group and in response to treatment with 1 μmol/L tamoxifen (Tam), 10 μmol/L tamoxifen, and 10 μmol/L 4-hydroxy-tamoxifen (4-H-Tam) treatment for 10 min and with 1 and 10 μmol/L tamoxifen treatment for 1 h in MCF-7 cells transfected with TRPV6. *, P < 0.05 treatment versus control group. More
Images
A.  Representative tracings showing changes in the calcium increase induced...
Published: 01 December 2009
FIGURE 10. A. Representative tracings showing changes in the calcium increase induced by inhibition of PKC with 200 nmol/L PMA for 10 min. B. Summary data showing the effect of the PKC activator PMA (200 nmol/L) and the PKC inhibitor calphostin C (100 nmol/L) on the inhibitory effect of 10 μmol/L tamoxifen in MCF-7 cells transfected with TRPV6. Cells were pretreated with calphostin C for 1 h. Data are the means of at least 25 cells from six separate experiments. *, P < 0.05 treatment versus control group. FIGURE 10. A. Representative tracings showing changes in the calcium increase induced by inhibition of PKC with 200 nmol/L PMA for 10 min. B. Summary data showing the effect of the PKC activator PMA (200 nmol/L) and the PKC inhibitor calphostin C (100 nmol/L) on the inhibitory effect of 10 μmol/L tamoxifen in MCF-7 cells transfected with TRPV6. Cells were pretreated with calphostin C for 1 h. Data are the means of at least 25 cells from six separate experiments. *, P < 0.05 treatment versus control group. More
Journal Articles
Mol Cancer Res (2006) 4 (3): 169–176.
Published: 01 March 2006
..., which are somewhat equivalent organs to prostate glands ( 20 ). Furthermore, we note that TLOC1/Sec62 protein is classified as a potential nonselective cation channel by the protein families database of alignments and that another nonselective cation channel, TRPV6, is overexpressed in human prostate...