Issues

EGFR-overexpressing A431 cells were transfected with PolyIC/MPPE at 0.1 mcg/mL, 24 hours later, 500,000 PBMCs per well were added to the cancer cells and coincubated for another 24 hours. Apoptotic cells (red fluorescence) were visualized using an Annexin-V-Biotin kit. To distinguish tumor cells from PBMCs, tumor cells were labeled with FITC-conjugated EGFR antibody (green fluorescence). Cells were visualized with a fluorescent microscope and photographed using a digital camera. For further details, please see Shir and coworkers on page 1033 in this issue.