Abstract A49: Induction of cell-cycle arrest and apoptosis in MCF7 cancer cells by Newcastle disease virus is associated with upregulation of p21^cip and p27^kip

Newcastle disease virus (NDV), a member of Paramyxoviridae family, is known for its selective oncogenic effects in cancer cells. NDV infections in cancer cells resulted in cell death through apoptosis. The actual mechanism of this induction has not been thoroughly investigated. Therefore, the objective of the present study is to investigate the effects of infection of a local velogenic strain of NDV, designated as AF2240, on the cell cycle regulation of MCF7 breast cancer cells. Infection was done at a multiplicity of infection of 1, and cell lysates were collected at 3, 6, 9, and 12 hours postinfection. Protein samples were subjected to SDS-PAGE and immunoblotting with antibodies for CDK, CDK inhibitors, and the cyclin family of cell cycle regulatory proteins. Results showed that proteins involved in cell cycle arrest, especially p21^cip and p27^kip, were significantly increased by 6 hours postinfection. These results suggest that NDV infection leads to initial cell cycle arrest, which is a first step towards cell death through apoptosis, in part via induction of the CDK inhibitors p21^cip and p27^kip. Findings from this study will provide further understanding of the mechanism of oncolysis by NDV.

Citation Information: Clin Cancer Res 2010;16(14 Suppl):A49.