In the article on PCI-24781 induces caspase and reactive oxygen species-dependent apoptosis published in the May 15, 2009 issue of Clinical Cancer Research, there was an error in Table 1. Down-regulated genes were incorrectly labeled as up-regulated genes. The correct table appears here.

Table 1.

Selected genes from expression analysis following 24-h treatment with PCI-24781, bortezomib, or the combination (in Ramos cells)

Accn #Down-regulated genes
0.25 μmol/L PCI/3 nmol/L Bor
NamePCI-24781BortezomibCombination*
Cell cycle-related     
    NM_000075 Cyclin-dependent kinase 4 (CDK4) 0.49 0.83 0.37 
    NM_001237 Cyclin A2 (CCNA2) 0.43 0.87 0.37 
    NM_001950 E2F transcription factor 4, p107/p130-binding (E2F4) 0.48 0.79 0.40 
    NM_001951 E2F transcription factor 5, p130-binding (E2F5) 0.46 0.98 0.43 
    NM_003903 CDC16 cell division cycle 16 homolog (S cerevisiae) (CDC16) 0.61 0.78 0.43 
    NM_031966 Cyclin B1 (CCNB1) 0.55 0.90 0.43 
    NM_001760 Cyclin D3 (CCND3) 0.48 1.02 0.46 
    NM_001255 CDC20 cell division cycle 20 homolog (S cerevisiae; CDC20) 0.61 0.82 0.46 
    NM_001262 Cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4; CDKN2C) 0.61 1.15 0.56 
    NM_001238 Cyclin E1 (CCNE1) 0.56 1.05 0.60 
    NM_001239 Cyclin H (CCNH) 0.74 0.90 0.64 
    NM_004701 Cyclin B2 (CCNB2) 0.90 0.95 0.67 
    NM_001240 Cyclin T1 (CCNT1) 0.67 1.16 0.71 
    NM_001761 Cyclin F (CCNF) 0.77 0.93 0.74 
    NM_001258 Cyclin-dependent kinase 3 (CDK3) 0.60 1.14 0.80 
    NM_004702 Cyclin E2 (CCNE2) 0.76 1.40 0.82 
    NM_001759 Cyclin D2 (CCND2) 0.85 0.87 0.86 
Proteasome     
    NM_017518 26S proteasome-associated UCH interacting protein 1 (UIP1) 0.36 0.79 0.29 
    NM_002811 Proteasome 26S subunit, non-ATPase, 7 (PSMD7) 0.68 1.44 0.57 
    NM_005047 Proteasome 26S subunit, non-ATPase, 5 (PSMD5) 0.61 1.04 0.70 
    NM_002812 Proteasome 26S subunit, non-ATPase, 8 (PSMD8) 0.60 1.13 0.70 
    NM_002794 Proteasome subunit, beta type, 2 (PSMB2) 0.75 1.26 0.83 
    NM_002790 Proteasome subunit, alpha type, 5 (PSMA5) 0.74 1.43 0.92 
Oxidative stress     
    NM_006440 Thioredoxin reductase 2 (TXNRD2) 0.36 0.83 0.24 
    NM_145177 Dehydrogenase/reductase (SDR family) X-linked (DHRSX) 0.37 0.87 0.40 
    NM_012473 Thioredoxin 2 (TXN2) 0.60 0.94 0.53 
    NM_145792 Microsomal glutathione S-transferase 1 (MGST1) 0.66 0.83 0.54 
    NM_002134 Heme oxygenase (decycling) 2 (HMOX2) 0.66 0.89 0.57 
    NM_012331 Methionine sulfoxide reductase A (MSRA) 0.74 0.86 0.72 
    NM_000637 Glutathione reductase (GSR) 0.61 1.46 0.80 
    NM_001752 Catalase (CAT) 0.93 0.72 0.83 
    NM_147149 Glutathione S-transferase M4 (GSTM4) 0.89 0.92 0.87 
    NM_182743 Thioredoxin reductase 1 (TXNRD1) 0.81 1.69 0.98 
HDACs and histones     
    NM_016596 Histone deacetylase 7A (HDAC7A) 0.35 1.06 0.31 
    NM_004964 Histone deacetylase 1 (HDAC1) 0.74 0.89 0.55 
        NM_018486 Histone deacetylase 8 (HDAC8) 0.76 1.02 0.64 
        NM_001527 Histone deacetylase 2 (HDAC2) 0.94 0.97 0.87 
NF-kB target genes     
        BC022556 Myc target 1 (MYCT1) 0.12 0.92 0.08 
        NM_001556 IkB kinase beta (IKBKB) 0.60 1.13 0.32 
    NM_032778 MYC induced nuclear antigen (MINA) 0.40 0.87 0.33 
    NM_002983 Chemokine (C-C motif) ligand 3 (CCL3) 0.46 0.85 0.42 
    NM_003998 NF-kB p105 (NFKB1) 0.47 1.19 0.43 
    NM_002467 v-myc viral oncogene homolog (MYC) 0.80 0.95 0.67 
    NM_006273 Chemokine (C-C motif) ligand 7 (CCL7) 0.51 0.61 0.69 
    NM_006509 Rel B, NFkB subunit (RELB) 0.32 1.27 0.72 
    NM_014002 IkB kinase ε(IKBKE) 0.59 0.88 0.76 
    NM_000600 Interleukin 6 (interferon, ρ2) (IL6) 0.84 1.28 0.81 
    NM_002503 IkB ρ(NFKBIB) 0.80 1.22 0.82 
    BC033522 Rel A, NFkB p65 subunit 0.92 1.04 0.85 
Apoptosis     
    NM_001168 Baculoviral IAP repeat-containing 5 (surviving; BIRC5) 0.56 0.83 0.54 
    NM_003879 CASP8 and FADD-like apoptosis regulator (CFLAR; c-FLIP) 0.60 1.03 0.56 
    NM_016252 Baculoviral IAP repeat-containing 6 (apollon; BIRC6) 0.75 1.10 0.72 
     
Accn # Up-regulated genes
 		0.25 μmol/L PCI/3 nmol/L Bor
 		  
 		Name
 		PCI-24781
 		Bortezomib
 		Combination*
 
Apoptosis and tumor necrosis factor receptor related     
    NM_000177 Gelsolin (GSN) 6.99 1.04 7.56 
    NM_032974 Caspase 10, apoptosis-related cysteine protease (CASP10) 7.99 0.81 6.16 
    NM_006573 Tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B) 3.59 1.91 3.38 
    NM_016639 Tumor necrosis factor receptor superfamily, member 12A (TNFRSF12A) 2.02 0.79 2.44 
    NM_018647 Tumor necrosis factor receptor superfamily, member 19 (TNFRSF19) -0.11 1.85 2.23 
    NM_003842 Tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B) 2.61 1.15 2.22 
    NM_014417 BCL2 binding component 3 (BBC3; PUMA) 0.95 1.69 1.85 
    NM_138621 BCL2-like 11 (apoptosis facilitator) (BCL2L11) 1.43 0.94 1.79 
    NM_000043 Fas (TNFRSF6; FAS) 1.72 1.46 1.73 
    NM_006290 Tumor necrosis factor, α-induced protein 3 (TNFAIP3) 1.35 0.95 1.69 
    NM_003300 TNF receptor-associated factor 3 (TRAF3) 2.80 0.78 1.62 
    NM_000594 Tumor necrosis factor (TNF superfamily, member 2; TNF) 0.99 0.94 1.31 
Cell cycle inhibitors     
    NM_000389 Cyclin-dependent kinase inhibitor 1A (p21, Cip1; CDKN1A) 25.87 0.92 28.45 
    NM_000076 Cyclin-dependent kinase inhibitor 1C (p57, Kip2; CDKN1C) 6.21 0.46 16.18 
    L36844 p15INK4B CDK inhibitory protein (CDKN2B) 5.26 2.54 4.49 
    NM_004064 Cyclin-dependent kinase inhibitor 1B (p27, Kip1; CDKN1B) 4.34 2.15 3.51 
Cytoskeletal     
    NM_178012 Tubulin, β 2B (TUBB2B) 14.66 0.87 16.45 
    BC056264 Histone 1, H2bg (HIST1H2BF) 6.42 1.17 5.65 
    NM_021052 Histone 1, H2ae (HIST1H2AE) 2.23 0.91 3.76 
    NM_021065 Histone 1, H2ad (HIST1H2AD) 1.47 0.69 2.69 
    NM_003543 Histone 1, H4h (HIST1H4H) 1.01 1.20 1.88 
    NM_001015053 Histone deacetylase 5 (HDAC5) 1.22 1.09 1.71 
    NM_003509 Histone 1, H2ai (HIST1H2AI) 1.12 0.80 1.45 
    NM_003516 Histone 2, H2aa (HIST2H2AA) 1.13 1.00 1.41 
    NM_003528 Histone 2, H2be (HIST2H2BE) 2.11 0.84 1.23 
Oxidative stress     
    NM_002738 Protein kinase C, β1 (PRKCB1) 14.00 0.86 11.85 
    NM_002133 Heme oxygenase (decycling) 1 (HMOX1) 6.52 1.82 7.53 
    NM_004417 Dual specificity phosphatase 1 (DUSP1) 3.49 0.67 5.54 
    NM_005346 Heat shock 70kDa protein 1B (HSPA1B) 2.23 3.74 5.27 
Other     
    NM_002206 Integrin, α7 (ITGA7) 15.21 1.47 15.01 
    NM_002228 v-jun sarcoma virus 17 oncogene homolog (JUN) 4.06 0.94 12.14 
    NM_000584 Interleukin 8 (IL8) 3.18 1.88 6.41 
    NM_000582 Osteopontin, early T-lymphocyte activation 1 (SPP1) 3.67 0.82 5.07 
    NM_004862 Lipopolysaccharide-induced TNF factor (LITAF) 2.26 1.20 2.08 
Accn #Down-regulated genes
0.25 μmol/L PCI/3 nmol/L Bor
NamePCI-24781BortezomibCombination*
Cell cycle-related     
    NM_000075 Cyclin-dependent kinase 4 (CDK4) 0.49 0.83 0.37 
    NM_001237 Cyclin A2 (CCNA2) 0.43 0.87 0.37 
    NM_001950 E2F transcription factor 4, p107/p130-binding (E2F4) 0.48 0.79 0.40 
    NM_001951 E2F transcription factor 5, p130-binding (E2F5) 0.46 0.98 0.43 
    NM_003903 CDC16 cell division cycle 16 homolog (S cerevisiae) (CDC16) 0.61 0.78 0.43 
    NM_031966 Cyclin B1 (CCNB1) 0.55 0.90 0.43 
    NM_001760 Cyclin D3 (CCND3) 0.48 1.02 0.46 
    NM_001255 CDC20 cell division cycle 20 homolog (S cerevisiae; CDC20) 0.61 0.82 0.46 
    NM_001262 Cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4; CDKN2C) 0.61 1.15 0.56 
    NM_001238 Cyclin E1 (CCNE1) 0.56 1.05 0.60 
    NM_001239 Cyclin H (CCNH) 0.74 0.90 0.64 
    NM_004701 Cyclin B2 (CCNB2) 0.90 0.95 0.67 
    NM_001240 Cyclin T1 (CCNT1) 0.67 1.16 0.71 
    NM_001761 Cyclin F (CCNF) 0.77 0.93 0.74 
    NM_001258 Cyclin-dependent kinase 3 (CDK3) 0.60 1.14 0.80 
    NM_004702 Cyclin E2 (CCNE2) 0.76 1.40 0.82 
    NM_001759 Cyclin D2 (CCND2) 0.85 0.87 0.86 
Proteasome     
    NM_017518 26S proteasome-associated UCH interacting protein 1 (UIP1) 0.36 0.79 0.29 
    NM_002811 Proteasome 26S subunit, non-ATPase, 7 (PSMD7) 0.68 1.44 0.57 
    NM_005047 Proteasome 26S subunit, non-ATPase, 5 (PSMD5) 0.61 1.04 0.70 
    NM_002812 Proteasome 26S subunit, non-ATPase, 8 (PSMD8) 0.60 1.13 0.70 
    NM_002794 Proteasome subunit, beta type, 2 (PSMB2) 0.75 1.26 0.83 
    NM_002790 Proteasome subunit, alpha type, 5 (PSMA5) 0.74 1.43 0.92 
Oxidative stress     
    NM_006440 Thioredoxin reductase 2 (TXNRD2) 0.36 0.83 0.24 
    NM_145177 Dehydrogenase/reductase (SDR family) X-linked (DHRSX) 0.37 0.87 0.40 
    NM_012473 Thioredoxin 2 (TXN2) 0.60 0.94 0.53 
    NM_145792 Microsomal glutathione S-transferase 1 (MGST1) 0.66 0.83 0.54 
    NM_002134 Heme oxygenase (decycling) 2 (HMOX2) 0.66 0.89 0.57 
    NM_012331 Methionine sulfoxide reductase A (MSRA) 0.74 0.86 0.72 
    NM_000637 Glutathione reductase (GSR) 0.61 1.46 0.80 
    NM_001752 Catalase (CAT) 0.93 0.72 0.83 
    NM_147149 Glutathione S-transferase M4 (GSTM4) 0.89 0.92 0.87 
    NM_182743 Thioredoxin reductase 1 (TXNRD1) 0.81 1.69 0.98 
HDACs and histones     
    NM_016596 Histone deacetylase 7A (HDAC7A) 0.35 1.06 0.31 
    NM_004964 Histone deacetylase 1 (HDAC1) 0.74 0.89 0.55 
        NM_018486 Histone deacetylase 8 (HDAC8) 0.76 1.02 0.64 
        NM_001527 Histone deacetylase 2 (HDAC2) 0.94 0.97 0.87 
NF-kB target genes     
        BC022556 Myc target 1 (MYCT1) 0.12 0.92 0.08 
        NM_001556 IkB kinase beta (IKBKB) 0.60 1.13 0.32 
    NM_032778 MYC induced nuclear antigen (MINA) 0.40 0.87 0.33 
    NM_002983 Chemokine (C-C motif) ligand 3 (CCL3) 0.46 0.85 0.42 
    NM_003998 NF-kB p105 (NFKB1) 0.47 1.19 0.43 
    NM_002467 v-myc viral oncogene homolog (MYC) 0.80 0.95 0.67 
    NM_006273 Chemokine (C-C motif) ligand 7 (CCL7) 0.51 0.61 0.69 
    NM_006509 Rel B, NFkB subunit (RELB) 0.32 1.27 0.72 
    NM_014002 IkB kinase ε(IKBKE) 0.59 0.88 0.76 
    NM_000600 Interleukin 6 (interferon, ρ2) (IL6) 0.84 1.28 0.81 
    NM_002503 IkB ρ(NFKBIB) 0.80 1.22 0.82 
    BC033522 Rel A, NFkB p65 subunit 0.92 1.04 0.85 
Apoptosis     
    NM_001168 Baculoviral IAP repeat-containing 5 (surviving; BIRC5) 0.56 0.83 0.54 
    NM_003879 CASP8 and FADD-like apoptosis regulator (CFLAR; c-FLIP) 0.60 1.03 0.56 
    NM_016252 Baculoviral IAP repeat-containing 6 (apollon; BIRC6) 0.75 1.10 0.72 
     
Accn # Up-regulated genes
 		0.25 μmol/L PCI/3 nmol/L Bor
 		  
 		Name
 		PCI-24781
 		Bortezomib
 		Combination*
 
Apoptosis and tumor necrosis factor receptor related     
    NM_000177 Gelsolin (GSN) 6.99 1.04 7.56 
    NM_032974 Caspase 10, apoptosis-related cysteine protease (CASP10) 7.99 0.81 6.16 
    NM_006573 Tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B) 3.59 1.91 3.38 
    NM_016639 Tumor necrosis factor receptor superfamily, member 12A (TNFRSF12A) 2.02 0.79 2.44 
    NM_018647 Tumor necrosis factor receptor superfamily, member 19 (TNFRSF19) -0.11 1.85 2.23 
    NM_003842 Tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B) 2.61 1.15 2.22 
    NM_014417 BCL2 binding component 3 (BBC3; PUMA) 0.95 1.69 1.85 
    NM_138621 BCL2-like 11 (apoptosis facilitator) (BCL2L11) 1.43 0.94 1.79 
    NM_000043 Fas (TNFRSF6; FAS) 1.72 1.46 1.73 
    NM_006290 Tumor necrosis factor, α-induced protein 3 (TNFAIP3) 1.35 0.95 1.69 
    NM_003300 TNF receptor-associated factor 3 (TRAF3) 2.80 0.78 1.62 
    NM_000594 Tumor necrosis factor (TNF superfamily, member 2; TNF) 0.99 0.94 1.31 
Cell cycle inhibitors     
    NM_000389 Cyclin-dependent kinase inhibitor 1A (p21, Cip1; CDKN1A) 25.87 0.92 28.45 
    NM_000076 Cyclin-dependent kinase inhibitor 1C (p57, Kip2; CDKN1C) 6.21 0.46 16.18 
    L36844 p15INK4B CDK inhibitory protein (CDKN2B) 5.26 2.54 4.49 
    NM_004064 Cyclin-dependent kinase inhibitor 1B (p27, Kip1; CDKN1B) 4.34 2.15 3.51 
Cytoskeletal     
    NM_178012 Tubulin, β 2B (TUBB2B) 14.66 0.87 16.45 
    BC056264 Histone 1, H2bg (HIST1H2BF) 6.42 1.17 5.65 
    NM_021052 Histone 1, H2ae (HIST1H2AE) 2.23 0.91 3.76 
    NM_021065 Histone 1, H2ad (HIST1H2AD) 1.47 0.69 2.69 
    NM_003543 Histone 1, H4h (HIST1H4H) 1.01 1.20 1.88 
    NM_001015053 Histone deacetylase 5 (HDAC5) 1.22 1.09 1.71 
    NM_003509 Histone 1, H2ai (HIST1H2AI) 1.12 0.80 1.45 
    NM_003516 Histone 2, H2aa (HIST2H2AA) 1.13 1.00 1.41 
    NM_003528 Histone 2, H2be (HIST2H2BE) 2.11 0.84 1.23 
Oxidative stress     
    NM_002738 Protein kinase C, β1 (PRKCB1) 14.00 0.86 11.85 
    NM_002133 Heme oxygenase (decycling) 1 (HMOX1) 6.52 1.82 7.53 
    NM_004417 Dual specificity phosphatase 1 (DUSP1) 3.49 0.67 5.54 
    NM_005346 Heat shock 70kDa protein 1B (HSPA1B) 2.23 3.74 5.27 
Other     
    NM_002206 Integrin, α7 (ITGA7) 15.21 1.47 15.01 
    NM_002228 v-jun sarcoma virus 17 oncogene homolog (JUN) 4.06 0.94 12.14 
    NM_000584 Interleukin 8 (IL8) 3.18 1.88 6.41 
    NM_000582 Osteopontin, early T-lymphocyte activation 1 (SPP1) 3.67 0.82 5.07 
    NM_004862 Lipopolysaccharide-induced TNF factor (LITAF) 2.26 1.20 2.08 

NOTE: Changes are expressed as ratios of treated to control cell levels.

*

Combination of PCI-24781 and bortezomib (0.25 μmol/L and 3 nmol/L, respectively).

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Bhalla S, Balasubramanian S, David K, et al. PCI-24781 induces caspase and reactive oxygen species-dependent apoptosis through NF-κB mechanisms and is synergistic with bortezomib in lymphoma cells. Clin Cancer Res 2009;15:3354–65.

View Original Article
American Association for Cancer Research
2009