SDF-1/CXCR4 and IP-10/CXCR3 expression in peripheral blood of breast cancer patients

A4

Background: Angiogenesis is an essential process for progression of solid tumors such as breast cancer. Chemokine and chemokine receptors closely correlate with this process for example Stromal cell-derived factor (SDF-1) and chemokine (C-X-C motif) receptor 4 (CXCR4) play important roles in recruitment of endothelial progenitor cells and angiogenesis. In contrast, interferon-inducible protein 10 (IP-10) and chemokine (C-X-C motif) receptor 3 (CXCR3) have angiostatic effects and might suppress tumor progression. Objective: This study examined the expressions of SDF-1, CXCR4, IP-10 and CXCR3 mRNA levels in peripheral blood and aimed to explore the significance of them between breast cancer patients and normal individuals.Methods: Sixty women with breast cancer were examined for expression of SDF-1, CXCR4, IP-10 and CXCR3 mRNA. For this, mRNA was extracted from peripheral blood and its expression was measured using real-time quantitative RT-PCR. Results were compared to those of a sex and age matched control group. Results: Relative Quantification (RQ) of SDF-1 and CXCR4 mRNAs were higher in patients than controls. In contrast, RQ of IP-10 mRNA was lower in patients than those in controls. As a result, CXCR4 were highly expressed and SDF-1 increased 2 fold in patients but IP-10 has shown 5 fold increased in controls. There were no any different in CXCR3 mRNA level between patients and controls. Conclusion: Differentially expressed SDF-1 and CXCR4 as angiogenic factors showed this chemokine and chemokine receptor as one of the factors increasing the susceptibility of women to breast cancer. In contrast, IP-10 expression might be suppressed the formation of the tumor.