Abstract
Moderate to high doses of ionizing radiation (IR) are known to increase the risk of cancer, particularly following childhood exposure. Concerns remain regarding risks from lower doses and the role of cancer-predisposing factors (CPF; genetic disorders, immunodeficiency, mutations/variants in DNA damage detection or repair genes) on radiation-induced cancer (RIC) risk. We conducted a systematic review of evidence that CPFs modify RIC risk in young people. Searches were performed in PubMed, Scopus, Web of Science, and EMBASE for epidemiologic studies of cancer risk in humans (<25 years) with a CPF, exposed to low–moderate IR. Risk of bias was considered. Fifteen articles focusing on leukemia, lymphoma, breast, brain, and thyroid cancers were included. We found inadequate evidence that CPFs modify the risk of radiation-induced leukemia, lymphoma, brain/central nervous system, and thyroid cancers and limited evidence that BRCA mutations modify radiation-induced breast cancer risk. Heterogeneity was observed across studies regarding exposure measures, and the numbers of subjects with CPFs other than BRCA mutations were very small. Further studies with more appropriate study designs are needed to elucidate the impact of CPFs on RIC. They should focus either on populations of carriers of specific gene mutations or on common susceptible variants using polygenic risk scores.
Introduction
Ionizing radiation (IR) plays an essential role in the diagnosis and follow-up of injuries, diseases, and the treatment of benign and malignant neoplasms (1). Medical sources of IR include conventional radiography, computed tomography (CT), nuclear medicine, fluoroscopy, and radiotherapy (RT).
Moderate to high doses of IR have been shown consistently to increase the risk of cancer in humans (2, 3), in particular in studies of cancer survivors where the radiation dose is related to an increased risk of second cancers in a number of different organs (4–8). One of the major concerns in radiation protection today, however, is characterizing and quantifying the effects of low doses (less than 100 mGy), as there is growing evidence that even low doses can increase cancer risk (3, 8–10), and identifying factors that may modify that risk, in particular age and genetic factors (3, 8, 11).
Medical applications of IR are a particular concern in radiation protection, particularly in children (8). In RT, though IR doses to the tumor target are very high (of the order of 50–60 Gy, to destroy cancer cells), distant organs receive low to moderate doses (100 mGy–500 Gy), since the dose decreases rapidly away from the target organ, and the long-term effects of these exposures on health, including second cancer risk, are very important to understand for the increasingly large populations of cancer survivors (5, 6, 8). Doses from diagnostic procedures are generally low, though repeated examinations, particularly from CT scanning, can lead to dose levels (hundreds of mGy) known to increase cancer risk. Because of the large numbers of persons undergoing diagnostic medical procedures every year, and the rapid development and implementation of new medical IR applications, medical diagnostic sources represent the largest source of general population IR exposure in high-income countries. It is therefore important to evaluate the potential impact of these exposures on risk of cancer in order to optimize practices and doses and reduce risks (6, 8).
Concerning factors that may modify the risk of radiation-induced cancer (RIC), it is well documented that exposure in childhood tends to confer a higher risk, compared with exposure later in life, especially in organs with high cell proliferation (6, 8). Genetic factors (8, 12), including mutations and variants in genes involved in detection or repair of DNA damage, in tumor-suppressor genes, and/or in proto-oncogenes may also play a role in RIC, conferring increased susceptibility (6, 8, 13). The link between cancer and radiation exposure (ultraviolet) in a population with mutations in DNA damage repair genes was first explored in the late 1960s among patients with xeroderma pigmentosum (XP) who developed skin cancer following sun exposure (6, 14). Persons with mutations in DNA damage repair genes, such as BRCA1 or 2 mutations, ataxia telangiectasia (AT; homozygous for a recessive AT mutation), and Nijmegen breakage syndrome (NBS; homozygous for an NBS mutation), are at increased risk of tissue damage following high levels of IR as these genes alter DNA repair, and heterozygous ATM and NBS mutation carriers may be at increased cancer risk (6, 11, 15). An increased risk of secondary cancers has also been reported among children with heritable retinoblastoma (RB), neurofibromatosis, and Li–Fraumeni syndrome (LFS) following moderate to high doses of RT treatments (11).
Children with increased susceptibility to RIC (genetic and/or related to immune deficiency) undergoing diagnostic or therapeutic radiation exposure are therefore of particular concern in radiation protection (6, 8). If such children can be identified, personalized screening, surveillance, management, and treatment can be offered to them to reduce their risk of RIC, including alternative diagnostic and treatment modalities (16, 17).
The objective of the current systematic review was to identify factors that may confer an increased susceptibility of RIC in subjects exposed during childhood, adolescence, and early adulthood (before the age of 25 years). Factors considered, termed cancer-predisposing factors (CPF) in this review, included conditions resulting from genetic disorders, immunodeficiency, mutation in DNA damage detection or repair genes, and family history of breast cancer.
Materials and Methods
The PRISMA-P 2015 checklist was used to formulate the systematic review protocol. The study was registered in PROSPERO (CRD4202014815; ref. 18).
Criteria for considering studies
An adaptation of the Cochrane Handbook for Systematic Reviews for observational studies was used as a study outline for the review (19). The Population, Exposure, Comparator, and Outcome and study design (PECO; Supplementary Table S1) framework was used to formulate the research question, assess eligibility, and synthesize each study (18–20).
The Population (P) of interest was children and young adults (<25 years) with a CPF. The list of conditions was compiled from a review by Journy and colleagues (21), Berrington de Gonzalez and colleagues (22), the BEIR VII report (6), and The Concise Handbook of Familial Cancer Susceptibility Syndromes (ref. 23; Table 1). The Exposure (E) was low–moderate doses of IR (<500 mGy) from medical, accidental, and environmental origins (including atomic bombings). Studies required an estimate of individual exposure or dose and an analysis of dose-/exposure-related response to allow the evaluation of the impact of the CPF on the risk of radiation-induced cancer. The Comparator (C) variable was excluded as it did not fit within the scope of the research aim. The Outcomes (O) of interest leukemia, breast, thyroid, and brain cancers and lymphoma. All but the latter are known to be associated with radiation exposure in childhood and adolescence (8). The evidence for lymphoma is less clear, though a number of large-scale recent studies have reported associations and changes in the classification of hematologic malignancies in recent decades have led to some leukemia subtypes being reclassified as lymphoma (21, 24–27).
. | Leukemia . | Lymphoma . | Brain . | Breast . | Thyroid . | Generica . | Studies . |
---|---|---|---|---|---|---|---|
Genetic disorders | |||||||
Li–Fraumeni syndrome | Yes | Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Fanconi anemia | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Langerhans cell histocytosis | Yes | Berrington de Gonzalez et al. 2016 | |||||
Kostmann granulocytopenia | Yes | Berrington de Gonzalez et al. 2016 | |||||
Nevoid basal cell syndrome | Yes | BEIR VII report 2006; Lindor et al. 2008; Berrington de Gonzalez et al. 2016 | |||||
Neurofibromatosis type 1 | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Neurofibromatosis type 2 | Yes | BEIR VII report 2006; Journy et al. 2015, 2016; Lindor et al. 2008; Berrington de Gonzalez et al. 2016 | |||||
Nijmegen breakage syndrome | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008 | ||||
Monosomy 7 (MDS) | Yes | Berrington de Gonzalez et al. 2016 | |||||
Mismatch-repair deficiency | Yes | Yes | Lindor et al. 2008 | ||||
Beckwith Wiedemann syndrome | Yes | Lindor et al. 2008 | |||||
Congenital heart defectsb | Yes | BEIR VII report 2006; Lindor et al. 2008 | |||||
Noonan syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Bloom syndrome | Yes | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||
Ataxia telangiectasia | Yes | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||
Shwachman–Diamond Syndrome | Yes | Berrington de Gonzalez et al. 2016 | |||||
Tuberous sclerosis | Yes | BEIR VII report 2006; Berrington de Gonzalez et al. 2016 | |||||
Von Hippel–Lindau | Yes | BEIR VII report 2006; Lindor et al. 2008; Berrington de Gonzalez et al. 2016 | |||||
Multiple endocrine neoplasia 1 (MEN 1) | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | |||||
Multiple endocrine neoplasia 2 (MEN 2) | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | |||||
Familial adenomatous polyposis | Yes | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | ||||
Turcot syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016; Lindor et al. 2008 | |||||
Down syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Xeroderma pigmentosum | Yes | Yes | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | |||
Klinefelter syndrome | Yes | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | ||||
Other phacomatoses | Yes | Yes | Journy et al. 2015, 2016 | ||||
Immunodeficiencies | |||||||
Primary immunodeficiency | Yes | Journy et al. 2015, 2016 | |||||
Severe combined immune deficiency (SCID) | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Common variable immune deficiency (CVID) | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
HIV/AIDS | Yes | Journy et al. 2015, 2016 | |||||
Wiskott–Aldrich syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Transplantation | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Mutations | |||||||
BRCA 1/2 gene | Yes | BEIR VII report 2006; Lindor et al. 2008 | |||||
A-T gene | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008 | ||||
Rb1 gene (retinoblastoma, retinocytoma) | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016 | ||||
TP53 | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016 | |||||
CHEK2 | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016 | |||||
XRCC1 | Yes | Yes | BEIR VII report 2006 |
. | Leukemia . | Lymphoma . | Brain . | Breast . | Thyroid . | Generica . | Studies . |
---|---|---|---|---|---|---|---|
Genetic disorders | |||||||
Li–Fraumeni syndrome | Yes | Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Fanconi anemia | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Langerhans cell histocytosis | Yes | Berrington de Gonzalez et al. 2016 | |||||
Kostmann granulocytopenia | Yes | Berrington de Gonzalez et al. 2016 | |||||
Nevoid basal cell syndrome | Yes | BEIR VII report 2006; Lindor et al. 2008; Berrington de Gonzalez et al. 2016 | |||||
Neurofibromatosis type 1 | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Neurofibromatosis type 2 | Yes | BEIR VII report 2006; Journy et al. 2015, 2016; Lindor et al. 2008; Berrington de Gonzalez et al. 2016 | |||||
Nijmegen breakage syndrome | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008 | ||||
Monosomy 7 (MDS) | Yes | Berrington de Gonzalez et al. 2016 | |||||
Mismatch-repair deficiency | Yes | Yes | Lindor et al. 2008 | ||||
Beckwith Wiedemann syndrome | Yes | Lindor et al. 2008 | |||||
Congenital heart defectsb | Yes | BEIR VII report 2006; Lindor et al. 2008 | |||||
Noonan syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Bloom syndrome | Yes | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||
Ataxia telangiectasia | Yes | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||
Shwachman–Diamond Syndrome | Yes | Berrington de Gonzalez et al. 2016 | |||||
Tuberous sclerosis | Yes | BEIR VII report 2006; Berrington de Gonzalez et al. 2016 | |||||
Von Hippel–Lindau | Yes | BEIR VII report 2006; Lindor et al. 2008; Berrington de Gonzalez et al. 2016 | |||||
Multiple endocrine neoplasia 1 (MEN 1) | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | |||||
Multiple endocrine neoplasia 2 (MEN 2) | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | |||||
Familial adenomatous polyposis | Yes | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | ||||
Turcot syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016; Lindor et al. 2008 | |||||
Down syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Xeroderma pigmentosum | Yes | Yes | Yes | BEIR VII report 2006; Journy et al. 2015, 2016 | |||
Klinefelter syndrome | Yes | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | ||||
Other phacomatoses | Yes | Yes | Journy et al. 2015, 2016 | ||||
Immunodeficiencies | |||||||
Primary immunodeficiency | Yes | Journy et al. 2015, 2016 | |||||
Severe combined immune deficiency (SCID) | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Common variable immune deficiency (CVID) | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
HIV/AIDS | Yes | Journy et al. 2015, 2016 | |||||
Wiskott–Aldrich syndrome | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Transplantation | Yes | Journy et al. 2015, 2016; Berrington de Gonzalez et al. 2016 | |||||
Mutations | |||||||
BRCA 1/2 gene | Yes | BEIR VII report 2006; Lindor et al. 2008 | |||||
A-T gene | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008 | ||||
Rb1 gene (retinoblastoma, retinocytoma) | Yes | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016 | ||||
TP53 | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016 | |||||
CHEK2 | Yes | BEIR VII report 2006; Lindor et al. 2008; Journy et al. 2015, 2016 | |||||
XRCC1 | Yes | Yes | BEIR VII report 2006 |
aNot specific to a particular cancer type.
bSelection based on increased exposure to medical IR and general increased cancer risk.
If the exposure under study was RT for a primary cancer, secondary malignancies were only considered, if they occurred in tissues/organs far from the exposure field with doses below 500 mGy. Empirical quantitative studies of cohort and case–control study design were selected. Exclusions included: non-English publications, systematic reviews, and case studies. All publications up to December 31, 2020, were considered.
Source of information
Databases used were PubMed, Scopus, Web of Science, and EMBASE. A manual search in reference lists from relevant articles was also performed.
Search strategy and study selection
The PECO structure was used to formulate the search strategy with “Population AND Exposure AND Outcome” conditions or string factors. A combination of free-text words and MeSH (Medical Subject Headings) terms was used to search the title, abstract, and keywords of the publications. The whole commands used with the search strings are provided in Supplementary Table S2. The literature search was conducted on November 6, 2019, and repeated on February 11, 2020. An additional search was performed on May 2, 2020, and December 30, 2020, to integrate some additional search terms deemed necessary for the scope of this review.
Study eligibility criteria (Supplementary Table S1) were defined by R. Harbron and M. Canet and tested on a subset sample of 10 randomly selected publications. A consistency test was performed to test interreviewer agreement; a Cohen's kappa (K) coefficient > 0.8 was considered sufficient in order to move to the following stage of the systematic review process. Studies were reviewed in parallel by M. Canet, R. Harbron, and E. Cardis following two phases: phase I, screening of titles and abstracts retrieved from the literature search; phase II, screening of full-text articles identified in phase I and of publications identified in the reference lists of relevant papers. Results from each reviewer were compared. Seven studies required further assessment by all three reviewers to reach consensus on inclusion/exclusion.
Record management
Data management relied on the open access online software program CADIMA (28). Data collection was performed by M. Canet and reviewed by R. Harbron, E. Cardis, and I. Thierry-Chef. Publications gathered from each database were uploaded and screened for potential duplicates. Following phase II, details on selected studies were collected, including study population, study characteristics, exposure assessment, outcome measurement, and information contributing to the risk assessment.
Risk of bias assessment
An adaptation of the Cochrane Collaboration's risk of bias tool developed for observational studies was used to assess risk of bias for each individual study (29–32).
The following sources of bias were evaluated: recruitment, blinding, exposure assessment, confounding, outcome assessment, selective reporting, conflict of interest, and any other bias. For each source, the risk of bias was rated as “low,” “probably low,” “probably high,” or “high.” The bias assessment was individually performed by M. Canet, R. Harbron, and E. Cardis, with further discussions in case of discrepancies. Studies in which a coauthor contributed were reviewed by other authors. An overall judgment of the risk of bias was performed to aid in the synthesis of the results and confidence characterization (30). For each identified bias, we assessed the extent it influenced the results and validity of each study (32, 33). In the event an identified bias was considered to compromise study results, the overall risk of bias judgment was “the study presents one [or more, according to the number of biases] threat to validity.”
Data synthesis and confidence characterization
Given the wide range of exposure measures and outcomes, a qualitative synthesis was carried out using the methods outlined in the Cochrane collaboration handbook and Popay and colleagues (19, 34). For each outcome, we reported the effect estimates from each study. We then used a standardized binary metric to estimate the direction of effect demonstrated across studies. Depending on the direction of effect IR exposure had on cancer risk estimates; either an ↑ suggested an increase effect, ↓ decrease in effect, and ✗ when no evidence of an effect was reported in each paper. To determine our confidence in the results from each study we used the GRADE-CerQual approach (35). Briefly, the assessment of each individual study was based on four components: (i) methodological limitations, (ii) coherence, (iii) adequacy of data, and (iv) relevance of studies according to PECO inclusion criteria; and later contributed to the overall assessment of confidence.
Results
Search results
Our primary search yielded 16,315 articles. Figure 1 shows the flow diagram for our selection of relevant studies. Following duplicate removal (n = 6,308), 10,005 articles were screened at title/abstract level, leaving 109 full texts for review. Ninety-four full-text articles were excluded (see Supplementary Table S3). Briefly, 30 articles were excluded based on the population criterion; 27 on the basis of study design; 12 did not fulfill our exposure criterion; 8 were excluded based on the population and outcome criterion; 5 for not fulfilling the exposure/outcome criterion; another 5 based on the population and exposure criteria; 3 articles were missing and 4 studies did not contain primary data or outcome. A total of 15 publications were finally included in this review, two of which described the same study (21, 36).
Study characteristics and risk of bias
Study characteristics are summarized in Table 2. There were eight cohorts and six case–control studies. Ten studies assessed the effects of diagnostic medical exposures: CT scanning in four and chest radiography in six. Three studies assessed therapeutic exposures and one accidental exposure. All studies considered exposures before age 25 years, five specifically below age 15. Nine studies included dose estimation and five assessed exposure based on the number of X-ray examinations only.
Methods . | . | Radiation exposure . | ||||||
---|---|---|---|---|---|---|---|---|
Reference . | Study population and location . | Study design . | Sample size . | Sex . | CPFs . | Type of exposure . | Age at exposure . | Dose distribution (mGy) . |
Diagnostic exposure | ||||||||
Andrieu et al. 2006 | BRCA1 and BRCA2 mutation carriers | Retrospective cohort | 1,601 mutation carriers: | Women only | BRCA1, BRCA2 | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
International BRCA1/2 Carrier Cohort Study (IBCCS)—UK, NL, FR, AUSTRIA, SWE, SPAIN, DK, BELG, IT, GER, ICEL, HUNG, CA | Pseudo-incident subcohort: 969 | Exposure metric is the number of X-rays. | ||||||
BRCA 1 mutation: 726 | Never exposed 36% | |||||||
BRCA 2 mutation: 243 | ≥1 period with 1–4 X-rays and none with ≥5 X-rays 33% | |||||||
≥1 period with ≥5 X-rays* 31% | ||||||||
*Breast dose unlikely to exceed 10–20 mGy. | ||||||||
Berrington de Gonzalez et al. 2016 | Children and adolescents who underwent CT scanning. | Retrospective Cohort | 180,000 | Women: 43.6% | Down, Noonan, Li–Fraumeni, Wiskott–Aldrich, Klinefelter, Shwachman–Diamond, Bloom, immunodeficiencies, nevoid basal cell, Turcot syndrome, Von Hippel–Lindau syndromes, Langerhans cell histiocytosis, Kostmann granulocytopenia, neurofibromatosis type 1 and type 2, ataxia telangiectasia, tuberous sclerosis, myelodysplasia monosomy, Fanconi anemia, solid organ, and bone marrow transplants | CT scans | <22 years | Dose range |
Great Britain | Men: 56.2% | 2.32–51.13 RBM dose | ||||||
0.25–330.18 Brain dose | ||||||||
Esther et al. 2007 | Individuals part of the Breast Cancer Family Registry (Breast CFR) | Nested case–control study | 2,254 cases: | Women only | Family history of BC | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
Canada (Ontario), Australia (Melbourne, Sydney) | 402 with family history | Exposure metric is number of X-rays | ||||||
1,704 without | ≥10 X-rays for tuberculosis or pneumonia | |||||||
3,431 controls | Family history 4 ca, 14 cont | |||||||
(1,556 unaffected sisters and 1,875 unrelated population controls) | without 17 ca, 14 cont | |||||||
≥10 Other X-rays | ||||||||
Family history 16 ca, 114 cont | ||||||||
without 60 ca, 114 cont | ||||||||
Esther et al. 2013 | BRCA 1 / 2 mutation carriers | Nested case–control study | BRCA1 carriers: | Women only | BRCA 1 and BRCA 2 | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
Cases: 167 | Distribution by number of X-rays. | |||||||
Controls: 287 | BRCA1 cases | |||||||
USA, Canada, Australia / New Zealand | BRCA2 carriers: | 0 X-ray 125 ca, 246 cont | ||||||
Cases: 104 | 1–2 11 ca, 16 cont | |||||||
Controls: 169 | 3–5 8 ca, 6 cont | |||||||
≥6 6 ca, 7 cont | ||||||||
Unknown 11 ca, 9 cont | ||||||||
BRCA2 cases | ||||||||
0 76 ca, 133 cont | ||||||||
1–2 7 ca, 15 cont | ||||||||
3–5 9 ca, 2 cont | ||||||||
≥6 3 ca, 3 cont | ||||||||
Unknown 5 ca, 12 cont | ||||||||
Gronwald et al. 2008 | Early-onset breast cancer patients. | Case–case study. | Cases: | Women only | BRCA 1 | Chest X-rays (excluding mammography) | <30 years | No estimation of dose |
Szczecin, Poland | 138 BC cases with BRCA 1 mutation | Mean number of chest X-rays before age 20 | ||||||
Controls: | 0.6 ca | |||||||
158 BC cases without | 0.3 cont | |||||||
Journy et al. 2015 | Children who underwent CT scanning | Retrospective Cohort | 67 274 | Women: 43% | Familial adenomatous polyposis, retinocytoma, multiple endocrine neoplasia (MEN1, MEN2), Fanconi anemia, ataxia telangiectasia, xeroderma pigmentosum, Bloom syndrome, neurofibromatosis type 1 and type 2, other phakomatoses, Noonan syndrome, Bloom syndrome, Down syndrome, Klinefelter, HIV/AIDS, severe combined immunodeficiency, Wiskott–Aldrich syndrome, common variable immunodeficiency, transplantation | CT scans | <10 years | Mean cumulative dose (sd) |
CPC of interest (n): | Men: 57% | 8.9 (10.7) RBM dose | ||||||
Journy et al. 2016 | France | 5 Leukemia CPF | 23.1 (31.8) Brain dose | |||||
7 Lymphoma CPF | ||||||||
7 Brain tumor CPF | ||||||||
Lecarpentier et al. 2011 | Carriers of BRCA1 or BRCA2 mutation | Retrospective Cohort | 1,337 mutation carriers | Women only | BRCA 1, BRCA 2 | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
Subcohort: 990 | Number of X-rays.: n (%). | |||||||
France | BRCA1 mutation: 635 | Never exposed: 10 (3) | ||||||
BRCA 2 mutation 355 | 1 period with 1–4 X-rays: 12 (3) | |||||||
Two periods with 1–4 X-rays: 276 (77) | ||||||||
≥1 period with 5+ X-rays: 62 (17) | ||||||||
Meulepas et al. 2019 | Children and adolescents who underwent CT scanning | Retrospective Cohort | 168,394 | Women: 46.1% | Tuberous sclerosis | CT scans | <18 years | Mean cumulative dose (IQR) |
CPC of interest (n): | Men: 53.9% | 9.5 (2.0–11.8) RBM dose | ||||||
Netherlands | Tuberous sclerosis (TSC) 82 | 38.5 (1.5–49.4) Brain dose | ||||||
Nikkilä et al. 2018 | General population | Registry-based case–control study | Cases: 1,093 | Women: 48% | Down syndrome | CT scans | <15 years | RBM dose (IQ) |
10.1 (4.79–13.6) ca | ||||||||
6.29 (5.69–7.14) cont | ||||||||
Controls: 3,279 | Men: 52% | |||||||
Finland | CPC of interest (n): | |||||||
Down syndrome: | ||||||||
Cases: 40 (3.5%) | ||||||||
Controls: 2 (0.1%) | ||||||||
Pijpe et al. 2012 | Carriers of BRCA1/2 mutations | Retrospective cohort study | 1,994 mutation carriers | Women only | BRCA 1, BRCA 2 | Conventional radiography of chest or shoulders, fluoroscopy, mammography | <20 years | Cumulative breast dose |
20–29 | <2 23% | |||||||
30–39 | 2–6.5 25% | |||||||
France, UK, Netherlands (overlap with Andrieu and Lecarpentier) | Subcohort: 1,122 | 6.6–17.3 28% | ||||||
BRCA 1 mutation: 685 | ≥17.3 24% | |||||||
BRCA 2 mutation: 437 | ||||||||
Therapeutic exposure | ||||||||
Kleinerman et al. 2005 | 1-year retinoblastoma survivors treated with radiotherapy | Retrospective cohort | Cohort sample size: 1,601 RB patients (following exclusion) | Women: 47.2% | Rb1 | Radiotherapy for retinoblastoma | <8 years | Mean breast dose |
Men: 52.8% | 400 mGy | |||||||
New York and Boston, US | Hereditary: 963 | |||||||
Nonhereditary: 638 | ||||||||
Little et al. 2014 | Retinoblastoma survivors | Retrospective case–control, nested within cohort | 31 cases (17 heritable RB) | Women: | Rb 1 | Radiotherapy for retinoblastoma | <15 years | Mean (range) breast bud dose |
US (Kleinerman cohort) and UK | 77 controls (42 heritable RB) | US: 97% | 160–170 (0–1,300) mGy | |||||
Controls matched on sex, RB heritable status, and birth date | UK: 100% | |||||||
Momani et al. 2004 | Patients treated with radiation for benign head and neck disorders | Cohort | Cohort sample size: | Women: 50% | Sibling concordance | Radiotherapy for treatment of benign disease | <16 years | Thyroid dose |
US | 4,296 treated for benign disorders | Men: 50% | 581 ± 210 Concordant pairs | |||||
251 sibling pairs | 679 ± 440 Discordant pairs | |||||||
Environmental exposure | ||||||||
Damiola et al. 2014 | General population | Retrospective case–control | 83 cases | Women: 61% | Polymorphisms in SNPs related to PTC, thyroid biology, or radiation-induced second primary tumors | 1,31I fall-out from Chernobyl accident | <15 years | Median thyroid dose (range) |
Gomel, Belarus | 324 controls | Men: 40% | 674 (14–4,921) ca | |||||
390 (2–6,003) cont |
Methods . | . | Radiation exposure . | ||||||
---|---|---|---|---|---|---|---|---|
Reference . | Study population and location . | Study design . | Sample size . | Sex . | CPFs . | Type of exposure . | Age at exposure . | Dose distribution (mGy) . |
Diagnostic exposure | ||||||||
Andrieu et al. 2006 | BRCA1 and BRCA2 mutation carriers | Retrospective cohort | 1,601 mutation carriers: | Women only | BRCA1, BRCA2 | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
International BRCA1/2 Carrier Cohort Study (IBCCS)—UK, NL, FR, AUSTRIA, SWE, SPAIN, DK, BELG, IT, GER, ICEL, HUNG, CA | Pseudo-incident subcohort: 969 | Exposure metric is the number of X-rays. | ||||||
BRCA 1 mutation: 726 | Never exposed 36% | |||||||
BRCA 2 mutation: 243 | ≥1 period with 1–4 X-rays and none with ≥5 X-rays 33% | |||||||
≥1 period with ≥5 X-rays* 31% | ||||||||
*Breast dose unlikely to exceed 10–20 mGy. | ||||||||
Berrington de Gonzalez et al. 2016 | Children and adolescents who underwent CT scanning. | Retrospective Cohort | 180,000 | Women: 43.6% | Down, Noonan, Li–Fraumeni, Wiskott–Aldrich, Klinefelter, Shwachman–Diamond, Bloom, immunodeficiencies, nevoid basal cell, Turcot syndrome, Von Hippel–Lindau syndromes, Langerhans cell histiocytosis, Kostmann granulocytopenia, neurofibromatosis type 1 and type 2, ataxia telangiectasia, tuberous sclerosis, myelodysplasia monosomy, Fanconi anemia, solid organ, and bone marrow transplants | CT scans | <22 years | Dose range |
Great Britain | Men: 56.2% | 2.32–51.13 RBM dose | ||||||
0.25–330.18 Brain dose | ||||||||
Esther et al. 2007 | Individuals part of the Breast Cancer Family Registry (Breast CFR) | Nested case–control study | 2,254 cases: | Women only | Family history of BC | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
Canada (Ontario), Australia (Melbourne, Sydney) | 402 with family history | Exposure metric is number of X-rays | ||||||
1,704 without | ≥10 X-rays for tuberculosis or pneumonia | |||||||
3,431 controls | Family history 4 ca, 14 cont | |||||||
(1,556 unaffected sisters and 1,875 unrelated population controls) | without 17 ca, 14 cont | |||||||
≥10 Other X-rays | ||||||||
Family history 16 ca, 114 cont | ||||||||
without 60 ca, 114 cont | ||||||||
Esther et al. 2013 | BRCA 1 / 2 mutation carriers | Nested case–control study | BRCA1 carriers: | Women only | BRCA 1 and BRCA 2 | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
Cases: 167 | Distribution by number of X-rays. | |||||||
Controls: 287 | BRCA1 cases | |||||||
USA, Canada, Australia / New Zealand | BRCA2 carriers: | 0 X-ray 125 ca, 246 cont | ||||||
Cases: 104 | 1–2 11 ca, 16 cont | |||||||
Controls: 169 | 3–5 8 ca, 6 cont | |||||||
≥6 6 ca, 7 cont | ||||||||
Unknown 11 ca, 9 cont | ||||||||
BRCA2 cases | ||||||||
0 76 ca, 133 cont | ||||||||
1–2 7 ca, 15 cont | ||||||||
3–5 9 ca, 2 cont | ||||||||
≥6 3 ca, 3 cont | ||||||||
Unknown 5 ca, 12 cont | ||||||||
Gronwald et al. 2008 | Early-onset breast cancer patients. | Case–case study. | Cases: | Women only | BRCA 1 | Chest X-rays (excluding mammography) | <30 years | No estimation of dose |
Szczecin, Poland | 138 BC cases with BRCA 1 mutation | Mean number of chest X-rays before age 20 | ||||||
Controls: | 0.6 ca | |||||||
158 BC cases without | 0.3 cont | |||||||
Journy et al. 2015 | Children who underwent CT scanning | Retrospective Cohort | 67 274 | Women: 43% | Familial adenomatous polyposis, retinocytoma, multiple endocrine neoplasia (MEN1, MEN2), Fanconi anemia, ataxia telangiectasia, xeroderma pigmentosum, Bloom syndrome, neurofibromatosis type 1 and type 2, other phakomatoses, Noonan syndrome, Bloom syndrome, Down syndrome, Klinefelter, HIV/AIDS, severe combined immunodeficiency, Wiskott–Aldrich syndrome, common variable immunodeficiency, transplantation | CT scans | <10 years | Mean cumulative dose (sd) |
CPC of interest (n): | Men: 57% | 8.9 (10.7) RBM dose | ||||||
Journy et al. 2016 | France | 5 Leukemia CPF | 23.1 (31.8) Brain dose | |||||
7 Lymphoma CPF | ||||||||
7 Brain tumor CPF | ||||||||
Lecarpentier et al. 2011 | Carriers of BRCA1 or BRCA2 mutation | Retrospective Cohort | 1,337 mutation carriers | Women only | BRCA 1, BRCA 2 | Chest X-rays (excluding mammography) | <20 and ≥20 | No estimation of dose |
Subcohort: 990 | Number of X-rays.: n (%). | |||||||
France | BRCA1 mutation: 635 | Never exposed: 10 (3) | ||||||
BRCA 2 mutation 355 | 1 period with 1–4 X-rays: 12 (3) | |||||||
Two periods with 1–4 X-rays: 276 (77) | ||||||||
≥1 period with 5+ X-rays: 62 (17) | ||||||||
Meulepas et al. 2019 | Children and adolescents who underwent CT scanning | Retrospective Cohort | 168,394 | Women: 46.1% | Tuberous sclerosis | CT scans | <18 years | Mean cumulative dose (IQR) |
CPC of interest (n): | Men: 53.9% | 9.5 (2.0–11.8) RBM dose | ||||||
Netherlands | Tuberous sclerosis (TSC) 82 | 38.5 (1.5–49.4) Brain dose | ||||||
Nikkilä et al. 2018 | General population | Registry-based case–control study | Cases: 1,093 | Women: 48% | Down syndrome | CT scans | <15 years | RBM dose (IQ) |
10.1 (4.79–13.6) ca | ||||||||
6.29 (5.69–7.14) cont | ||||||||
Controls: 3,279 | Men: 52% | |||||||
Finland | CPC of interest (n): | |||||||
Down syndrome: | ||||||||
Cases: 40 (3.5%) | ||||||||
Controls: 2 (0.1%) | ||||||||
Pijpe et al. 2012 | Carriers of BRCA1/2 mutations | Retrospective cohort study | 1,994 mutation carriers | Women only | BRCA 1, BRCA 2 | Conventional radiography of chest or shoulders, fluoroscopy, mammography | <20 years | Cumulative breast dose |
20–29 | <2 23% | |||||||
30–39 | 2–6.5 25% | |||||||
France, UK, Netherlands (overlap with Andrieu and Lecarpentier) | Subcohort: 1,122 | 6.6–17.3 28% | ||||||
BRCA 1 mutation: 685 | ≥17.3 24% | |||||||
BRCA 2 mutation: 437 | ||||||||
Therapeutic exposure | ||||||||
Kleinerman et al. 2005 | 1-year retinoblastoma survivors treated with radiotherapy | Retrospective cohort | Cohort sample size: 1,601 RB patients (following exclusion) | Women: 47.2% | Rb1 | Radiotherapy for retinoblastoma | <8 years | Mean breast dose |
Men: 52.8% | 400 mGy | |||||||
New York and Boston, US | Hereditary: 963 | |||||||
Nonhereditary: 638 | ||||||||
Little et al. 2014 | Retinoblastoma survivors | Retrospective case–control, nested within cohort | 31 cases (17 heritable RB) | Women: | Rb 1 | Radiotherapy for retinoblastoma | <15 years | Mean (range) breast bud dose |
US (Kleinerman cohort) and UK | 77 controls (42 heritable RB) | US: 97% | 160–170 (0–1,300) mGy | |||||
Controls matched on sex, RB heritable status, and birth date | UK: 100% | |||||||
Momani et al. 2004 | Patients treated with radiation for benign head and neck disorders | Cohort | Cohort sample size: | Women: 50% | Sibling concordance | Radiotherapy for treatment of benign disease | <16 years | Thyroid dose |
US | 4,296 treated for benign disorders | Men: 50% | 581 ± 210 Concordant pairs | |||||
251 sibling pairs | 679 ± 440 Discordant pairs | |||||||
Environmental exposure | ||||||||
Damiola et al. 2014 | General population | Retrospective case–control | 83 cases | Women: 61% | Polymorphisms in SNPs related to PTC, thyroid biology, or radiation-induced second primary tumors | 1,31I fall-out from Chernobyl accident | <15 years | Median thyroid dose (range) |
Gomel, Belarus | 324 controls | Men: 40% | 674 (14–4,921) ca | |||||
390 (2–6,003) cont |
Synthesis of evidence/results
Eight studies assessed the effects of CPFs on the IR-related breast cancer risk; three studied leukemia risk, three brain tumor risk, two studies thyroid cancer, and one lymphoma. Table 3 summarizes the results of the studies.
Reference . | Age at outcome (years) . | Outcome . | Summary of results . | Comments . | Direction of effect . |
---|---|---|---|---|---|
Diagnostic exposure | |||||
Andrieu et al. 2016 | >18 | Breast cancer | Pseudo-incident cohort—compared with nonexposed | Adjusted for parity and oophorectomy (yes/no), stratified by country group. | ↑ |
Mean subcohort: 41 | HR 1.76 (0.9–3.4) – ≥ 1 period 1–4 X-rays 50 cases | ||||
HR 2.69 (1.4–5.3) – ≥ 1 one period 5+ 76 cases | Adjusted for surveillance bias. | ||||
HR 5.21 (1.6–17.5)—Exp before age 20 only 12 cases | |||||
HR 1.91 (0.9 –4.1)—Exp after age 20 only 26 cases | |||||
Berrington de Gonzalez et al. 2016 | All | Brain tumors | ERR 0.023/mGy (0.010–0.049)—overall 135 cases | Adjusted for sex, attained age, age at exposure, SES. | ✗ |
ERR 0.027/mGy (0.010–0.065)—excluding brain tumor-related conditions 106 cases | Doses lagged by 2 years for leukemia and 5 years for brain tumors | ||||
Leukemia / MDS | ERR 0.036/mGy (0.005–0.118)—overall 74 cases | ✗ | |||
ERR 0.034/mGy (0.004–0.116)—excluding leukemia-related conditions /PF 67 cases | |||||
Esther et al. 2007 | 18–69 | Breast cancer | Cases with a family history of breast cancer vs. all controls | Adjusted for age (<35, 35–44, 45–54, ≥55), study center (Ontario, Australia), country of birth (same as study center: yes, no), education (high school graduate or less, some college or vocational school, university degree). | ✗ |
X-ray for TB or pneumonia | |||||
OR 2.27 (1.37–3.76): < 10 X-rays 21 cases, 80 controls | |||||
OR 2.75 (0.89–8.53): ≥ 10 X-rays 4 cases, 14 controls | |||||
OR 2.61 (1.51–4.51): age 1st exp < 20 18 cases, 64 controls | |||||
Other X-rays | |||||
OR 0.78 (0.60–1.01): < 10 X-rays 86 cases, 804 controls | Similar results when adjusting for personal history of benign breast disease, age at menarche, number of full-term pregnancies, age at first full-term pregnancy, menopausal status, oral contraception, hormonal therapy, alcohol consumption, and smoking. | ||||
OR 1.07 (0.62–1.85): ≥ 10 X-rays 16 cases, 114 controls | |||||
OR 1.21 (0.86–1.70): age 1st exp < 20 49 cases, 295 controls | |||||
Cases without a family history of breast cancer vs. all controls | |||||
X-ray for TB or pneumonia | |||||
OR 2.18 (1.57–3.02): < 10 X-rays 81 cases, 80 controls | |||||
OR 2.35 (1.13–4.89): ≥ 10 X-rays 17 cases, 14 controls | |||||
OR 2.51 (1.76–3.60): age 1st exp < 20 71 cases, 64 controls | Majority of cases had first exposure after 20 years of age. | ||||
Other X-rays | |||||
OR 0.72 (0.61–0.84): < 10 X-rays 294 cases, 804 controls | |||||
OR 1.21 (0.89–1.69): ≥ 10 X-rays 60 cases, 114 controls | |||||
OR 1.04 (0.83–1.30): age 1st exp< 20 143 cases, 295 controls | |||||
Esther et al. 2013 | <50 | Breast cancer | BRCA 1 mutation carriers | Adjusted for reference age (<40 and 40–49 years), country/region, history of breast or ovarian cancer in first-degree relatives (yes, no), and number of full-term pregnancies). Subcohort: carriers diagnosed with breast cancer/censored within five years before questionnaire completion, follow-up counted only for this five-year period. | ✗ |
OR 1.0: No chest X-rays 125 cases, 246 controls | |||||
OR 0.84 (0.33–2.13): 1–2 chest X-rays 11 cases, 16 controls | |||||
OR 1.22 (0.35–4.21): 3–5 chest X-rays 8 cases, 6 controls | |||||
OR 1.20 (0.37–3.96): ≥ 6 chest X-rays 6 cases, 7 controls | |||||
OR 1.75 (0.57–5.35): unknown number 11 cases, 9 controls | Majority of cases had first exposure after 20 years of age. | ||||
OR 0.57 (0.22–1.48): age 1st exp < 20 years 10 cases, 22 controls | |||||
OR 1.69 (0.76–3.76): age 1st exp ≥ 20 years 21 cases, 14 controls | |||||
OR 2.58 (0.42–15.8): unknown age 1st exp 5 cases, 2 controls | |||||
BRCA 2 mutation carriers | |||||
OR 1.0: No chest X-rays 76 cases, 133 controls | |||||
OR 0.80 (0.28–2.28): 1–2 chest X-rays 7 cases, 15 controls | |||||
OR 10.63 (1.93–58): 3–5 chest X-rays 9 cases, 2 controls | |||||
OR 0.89 (0.15–5.49): ≥ 6 chest X-rays 3 cases, 3 controls | |||||
OR 0.62 (0.18–2.17): unknown number 5 cases, 12 controls | |||||
OR 1.55 (0.54–4.47): age 1st exp < 20 years | |||||
9 cases, 10 controls | |||||
OR 1.06 (0.43–2.60): age 1st exp ≥ 20 years 11 cases, 17 controls | |||||
OR 1.12 (0.24–5.26): unknown age 1st exp 4 cases, 5 controls | |||||
Gronwald et al. 2008 | <50 | Breast cancer | Carriers (BRCA1) vs. noncarriers | Adjusted for birth year, age at diagnosis. | ↑ |
OR 1.8 (1.0–3.2): 1 chest X-ray < 20 years numbers not specified | |||||
OR 5.7 (1.2–27): 4+chest X-rays < 20 years | |||||
Journy et al. 2015 | Children | CNS Cancer | ERR 0.022/mGy (−0.016; 0.061): overall 22 cases | Adjusted for sex, period of birth, attained age and time since entry in cohort, presence of any PF (yes/no) or presence of specific PF. Lag period of 1–4 years was tested. | ✗ |
<15 | ERR 0.028/mGy: excluding CNS PF 15 cases | ||||
Leukemia | ERR 0.057/mGy (−0.079; 0.193): overall 17 cases | ↓ | |||
ERR 0.187/mGy: excluding leukemia PF 12 cases | |||||
Lymphoma | ERR 0.018/mGy (−0.068; 0.104): overall 19 cases | ✗ | |||
ERR 0.025/mGy: excluding lymphoma PF 12 cases | |||||
Journy et al. 2016 | Children | CNS tumor | HR 1.07/10 mGy (0.99–1.10): no PF 15 cases | Adjustment for sex and age at cohort entry. | ✗ |
<15 | HR 0.80/10 mGy (0.45–1.06): with a PF 7 cases | ||||
Interaction P value 0.22 | |||||
Leukemia | HR 1.16/10 mGy (0.77–1.27): no PF 12 cases | ✗ | |||
HR 0.57/10 mGy (0.06–1.32): with a PF 5 cases | |||||
Interaction P value: 0.42 | |||||
Lecarpentier et al. 2011 | >18 | Breast cancer | HR exposed vs. nonexposed | Adjusted for menopausal status (yes/no), parity, BMI, and gene. | ✗ |
Mean: 44 | 4.83 (1.83-12.8) one period with 1–4 X-rays 12 cases | 90% cases had 1st exposure before age 20 | |||
6.22 (2.94-13.1) two periods with 1–4 X-rays 276 cases | |||||
2.80 (1.30–6.05) one period with 5+ X-rays 62 cases | |||||
4.16 (2.03–8.56): age at 1st exp. < 20 years 331 cases | |||||
6.45 (2.86–14.6): age at 1st exp. ≥ 20 years 34 cases | |||||
Meulepas et al. 2019 | Children, adolescents, and adults | Brain tumors | Overall brain tumor risk | Adjusted for age, sex, and calendar period, quartiles of income, and house value. | ✗ |
ERR 0.0086 mGy (0.002–0.022) 84 cases | |||||
NA | Exclusion of TSC subjects did not change risk estimate (not shown) | Dose lagged by 5 years. | |||
ERR 0.0079/mGy (0.002–0.021) 81 cases | |||||
Nikkilä et al. 2018 | <15 | Leukemia | Overall Leukemia risk (NCICT dosimetry) | Adjustments for large for gestational age, maternal smoking during pregnancy, parental education, and parental socioeconomic status. | ✗ |
EOR 0.13/mGy (0.02-0.26) 15 cases, 10 controls | |||||
exposed | Dose lagged by 2 years. | ||||
Down syndrome–radiation Interaction P value: 0.99 (risk estimate not shown: 5 cases of Down Syndrome) | |||||
Pijpe et al. 2012 | >18 | Breast cancer | Exposed < 20 years | Adjusted for age at entry in subcohort, parity, and menopause and clustered on family. | ↑ |
HR 1.47 (0.89–2.42) <2 mGy 31 cases | |||||
Mean: subcohort 41 | HR 1.09 (0.41–2.91) 2–6.5 mGy 6 cases | ||||
HR 3.16 (1.19–8.36) 6.6 mGy+ 12 cases | |||||
Therapeutic exposure | |||||
Kleinerman et al. 2005 | Adults ≥ 40 | Breast cancer | Hereditary RB survivors vs. general population | Adjusted for age, sex, and calendar period. | ✗ |
SIR 3.3 (0.4–12): no radiation exposure 2 cases | |||||
SIR 4.2 (1.8–8.2): moderate exposure 8 cases | |||||
Overall SIRs: without taking radiation into account | |||||
SIR 3.96 (1.9–7.3) hereditary RB | |||||
SIR 2.84 (1.1–5.9) nonhereditary RB without taking radiation into account | |||||
Little et al. 2014 | Young adults and adults | Breast cancer | OR at 100 mGy | Adjusted for chemotherapy (US cohort) and bilateral blindness. | ↓ |
1.96 (1.06–inf) nonhereditary RB 8 cases and 0 controls | |||||
Range (mean): 25–56.1 (41.9) | 0.80 (0.58–1.04) hereditary RB 24 cases and 64 controls | ||||
heterogeneity p- value 0.026 | |||||
Momani et al. 2004 | Young adults and adults | Thyroid cancer | No difference from expected 20: concordant pairs | ✗ | |
Mean (SD): 30.9 (10.1) | 63: discordant pairs | ||||
Environmental exposure | |||||
Damiola et al. 2014 | Children, adolescents, and young adults <25 years | Papillary thyroid carcinoma | OR 0.34/mGy (0.16–0.73) ATM (D1853N) | Adjusted for age and sex through matching as well as iodine deficiency and supplementation. | ✗ |
LRT interaction P value 0.45 | |||||
OR 0.95/mGy (0.40–2.29) ATM P1526P (rs1800889) LRT interaction P value 0.02: ns when adjusted for multiple comparison | |||||
OR 1.55/mGy (1.03–2.34) FOXE1 5′UTR (rs1867277) LRT interaction P value 0.53 |
Reference . | Age at outcome (years) . | Outcome . | Summary of results . | Comments . | Direction of effect . |
---|---|---|---|---|---|
Diagnostic exposure | |||||
Andrieu et al. 2016 | >18 | Breast cancer | Pseudo-incident cohort—compared with nonexposed | Adjusted for parity and oophorectomy (yes/no), stratified by country group. | ↑ |
Mean subcohort: 41 | HR 1.76 (0.9–3.4) – ≥ 1 period 1–4 X-rays 50 cases | ||||
HR 2.69 (1.4–5.3) – ≥ 1 one period 5+ 76 cases | Adjusted for surveillance bias. | ||||
HR 5.21 (1.6–17.5)—Exp before age 20 only 12 cases | |||||
HR 1.91 (0.9 –4.1)—Exp after age 20 only 26 cases | |||||
Berrington de Gonzalez et al. 2016 | All | Brain tumors | ERR 0.023/mGy (0.010–0.049)—overall 135 cases | Adjusted for sex, attained age, age at exposure, SES. | ✗ |
ERR 0.027/mGy (0.010–0.065)—excluding brain tumor-related conditions 106 cases | Doses lagged by 2 years for leukemia and 5 years for brain tumors | ||||
Leukemia / MDS | ERR 0.036/mGy (0.005–0.118)—overall 74 cases | ✗ | |||
ERR 0.034/mGy (0.004–0.116)—excluding leukemia-related conditions /PF 67 cases | |||||
Esther et al. 2007 | 18–69 | Breast cancer | Cases with a family history of breast cancer vs. all controls | Adjusted for age (<35, 35–44, 45–54, ≥55), study center (Ontario, Australia), country of birth (same as study center: yes, no), education (high school graduate or less, some college or vocational school, university degree). | ✗ |
X-ray for TB or pneumonia | |||||
OR 2.27 (1.37–3.76): < 10 X-rays 21 cases, 80 controls | |||||
OR 2.75 (0.89–8.53): ≥ 10 X-rays 4 cases, 14 controls | |||||
OR 2.61 (1.51–4.51): age 1st exp < 20 18 cases, 64 controls | |||||
Other X-rays | |||||
OR 0.78 (0.60–1.01): < 10 X-rays 86 cases, 804 controls | Similar results when adjusting for personal history of benign breast disease, age at menarche, number of full-term pregnancies, age at first full-term pregnancy, menopausal status, oral contraception, hormonal therapy, alcohol consumption, and smoking. | ||||
OR 1.07 (0.62–1.85): ≥ 10 X-rays 16 cases, 114 controls | |||||
OR 1.21 (0.86–1.70): age 1st exp < 20 49 cases, 295 controls | |||||
Cases without a family history of breast cancer vs. all controls | |||||
X-ray for TB or pneumonia | |||||
OR 2.18 (1.57–3.02): < 10 X-rays 81 cases, 80 controls | |||||
OR 2.35 (1.13–4.89): ≥ 10 X-rays 17 cases, 14 controls | |||||
OR 2.51 (1.76–3.60): age 1st exp < 20 71 cases, 64 controls | Majority of cases had first exposure after 20 years of age. | ||||
Other X-rays | |||||
OR 0.72 (0.61–0.84): < 10 X-rays 294 cases, 804 controls | |||||
OR 1.21 (0.89–1.69): ≥ 10 X-rays 60 cases, 114 controls | |||||
OR 1.04 (0.83–1.30): age 1st exp< 20 143 cases, 295 controls | |||||
Esther et al. 2013 | <50 | Breast cancer | BRCA 1 mutation carriers | Adjusted for reference age (<40 and 40–49 years), country/region, history of breast or ovarian cancer in first-degree relatives (yes, no), and number of full-term pregnancies). Subcohort: carriers diagnosed with breast cancer/censored within five years before questionnaire completion, follow-up counted only for this five-year period. | ✗ |
OR 1.0: No chest X-rays 125 cases, 246 controls | |||||
OR 0.84 (0.33–2.13): 1–2 chest X-rays 11 cases, 16 controls | |||||
OR 1.22 (0.35–4.21): 3–5 chest X-rays 8 cases, 6 controls | |||||
OR 1.20 (0.37–3.96): ≥ 6 chest X-rays 6 cases, 7 controls | |||||
OR 1.75 (0.57–5.35): unknown number 11 cases, 9 controls | Majority of cases had first exposure after 20 years of age. | ||||
OR 0.57 (0.22–1.48): age 1st exp < 20 years 10 cases, 22 controls | |||||
OR 1.69 (0.76–3.76): age 1st exp ≥ 20 years 21 cases, 14 controls | |||||
OR 2.58 (0.42–15.8): unknown age 1st exp 5 cases, 2 controls | |||||
BRCA 2 mutation carriers | |||||
OR 1.0: No chest X-rays 76 cases, 133 controls | |||||
OR 0.80 (0.28–2.28): 1–2 chest X-rays 7 cases, 15 controls | |||||
OR 10.63 (1.93–58): 3–5 chest X-rays 9 cases, 2 controls | |||||
OR 0.89 (0.15–5.49): ≥ 6 chest X-rays 3 cases, 3 controls | |||||
OR 0.62 (0.18–2.17): unknown number 5 cases, 12 controls | |||||
OR 1.55 (0.54–4.47): age 1st exp < 20 years | |||||
9 cases, 10 controls | |||||
OR 1.06 (0.43–2.60): age 1st exp ≥ 20 years 11 cases, 17 controls | |||||
OR 1.12 (0.24–5.26): unknown age 1st exp 4 cases, 5 controls | |||||
Gronwald et al. 2008 | <50 | Breast cancer | Carriers (BRCA1) vs. noncarriers | Adjusted for birth year, age at diagnosis. | ↑ |
OR 1.8 (1.0–3.2): 1 chest X-ray < 20 years numbers not specified | |||||
OR 5.7 (1.2–27): 4+chest X-rays < 20 years | |||||
Journy et al. 2015 | Children | CNS Cancer | ERR 0.022/mGy (−0.016; 0.061): overall 22 cases | Adjusted for sex, period of birth, attained age and time since entry in cohort, presence of any PF (yes/no) or presence of specific PF. Lag period of 1–4 years was tested. | ✗ |
<15 | ERR 0.028/mGy: excluding CNS PF 15 cases | ||||
Leukemia | ERR 0.057/mGy (−0.079; 0.193): overall 17 cases | ↓ | |||
ERR 0.187/mGy: excluding leukemia PF 12 cases | |||||
Lymphoma | ERR 0.018/mGy (−0.068; 0.104): overall 19 cases | ✗ | |||
ERR 0.025/mGy: excluding lymphoma PF 12 cases | |||||
Journy et al. 2016 | Children | CNS tumor | HR 1.07/10 mGy (0.99–1.10): no PF 15 cases | Adjustment for sex and age at cohort entry. | ✗ |
<15 | HR 0.80/10 mGy (0.45–1.06): with a PF 7 cases | ||||
Interaction P value 0.22 | |||||
Leukemia | HR 1.16/10 mGy (0.77–1.27): no PF 12 cases | ✗ | |||
HR 0.57/10 mGy (0.06–1.32): with a PF 5 cases | |||||
Interaction P value: 0.42 | |||||
Lecarpentier et al. 2011 | >18 | Breast cancer | HR exposed vs. nonexposed | Adjusted for menopausal status (yes/no), parity, BMI, and gene. | ✗ |
Mean: 44 | 4.83 (1.83-12.8) one period with 1–4 X-rays 12 cases | 90% cases had 1st exposure before age 20 | |||
6.22 (2.94-13.1) two periods with 1–4 X-rays 276 cases | |||||
2.80 (1.30–6.05) one period with 5+ X-rays 62 cases | |||||
4.16 (2.03–8.56): age at 1st exp. < 20 years 331 cases | |||||
6.45 (2.86–14.6): age at 1st exp. ≥ 20 years 34 cases | |||||
Meulepas et al. 2019 | Children, adolescents, and adults | Brain tumors | Overall brain tumor risk | Adjusted for age, sex, and calendar period, quartiles of income, and house value. | ✗ |
ERR 0.0086 mGy (0.002–0.022) 84 cases | |||||
NA | Exclusion of TSC subjects did not change risk estimate (not shown) | Dose lagged by 5 years. | |||
ERR 0.0079/mGy (0.002–0.021) 81 cases | |||||
Nikkilä et al. 2018 | <15 | Leukemia | Overall Leukemia risk (NCICT dosimetry) | Adjustments for large for gestational age, maternal smoking during pregnancy, parental education, and parental socioeconomic status. | ✗ |
EOR 0.13/mGy (0.02-0.26) 15 cases, 10 controls | |||||
exposed | Dose lagged by 2 years. | ||||
Down syndrome–radiation Interaction P value: 0.99 (risk estimate not shown: 5 cases of Down Syndrome) | |||||
Pijpe et al. 2012 | >18 | Breast cancer | Exposed < 20 years | Adjusted for age at entry in subcohort, parity, and menopause and clustered on family. | ↑ |
HR 1.47 (0.89–2.42) <2 mGy 31 cases | |||||
Mean: subcohort 41 | HR 1.09 (0.41–2.91) 2–6.5 mGy 6 cases | ||||
HR 3.16 (1.19–8.36) 6.6 mGy+ 12 cases | |||||
Therapeutic exposure | |||||
Kleinerman et al. 2005 | Adults ≥ 40 | Breast cancer | Hereditary RB survivors vs. general population | Adjusted for age, sex, and calendar period. | ✗ |
SIR 3.3 (0.4–12): no radiation exposure 2 cases | |||||
SIR 4.2 (1.8–8.2): moderate exposure 8 cases | |||||
Overall SIRs: without taking radiation into account | |||||
SIR 3.96 (1.9–7.3) hereditary RB | |||||
SIR 2.84 (1.1–5.9) nonhereditary RB without taking radiation into account | |||||
Little et al. 2014 | Young adults and adults | Breast cancer | OR at 100 mGy | Adjusted for chemotherapy (US cohort) and bilateral blindness. | ↓ |
1.96 (1.06–inf) nonhereditary RB 8 cases and 0 controls | |||||
Range (mean): 25–56.1 (41.9) | 0.80 (0.58–1.04) hereditary RB 24 cases and 64 controls | ||||
heterogeneity p- value 0.026 | |||||
Momani et al. 2004 | Young adults and adults | Thyroid cancer | No difference from expected 20: concordant pairs | ✗ | |
Mean (SD): 30.9 (10.1) | 63: discordant pairs | ||||
Environmental exposure | |||||
Damiola et al. 2014 | Children, adolescents, and young adults <25 years | Papillary thyroid carcinoma | OR 0.34/mGy (0.16–0.73) ATM (D1853N) | Adjusted for age and sex through matching as well as iodine deficiency and supplementation. | ✗ |
LRT interaction P value 0.45 | |||||
OR 0.95/mGy (0.40–2.29) ATM P1526P (rs1800889) LRT interaction P value 0.02: ns when adjusted for multiple comparison | |||||
OR 1.55/mGy (1.03–2.34) FOXE1 5′UTR (rs1867277) LRT interaction P value 0.53 |
Strong heterogeneity was observed across studies regarding exposure measures and risk estimates. The risk of bias assessment is summarized in Fig. 2 and Supplementary Table S4.
A summary of the evidence and evaluation of the possible modifying effects of CPFs is provided, by outcome, in Table 4.
. | . | Elements for assessing the certainty in the evidence . | . | . | |
---|---|---|---|---|---|
Summary of review finding . | Studies contributing to the review finding . | Factors that decrease confidence . | Factors that increase confidence . | CERQual assessment of confidence in the evidence . | Explanation of CERQual assessment . |
Leukemia | |||||
No indication of a greater leukemia risk following low–moderate IR exposure, between individuals with a leukemia CPF and without. | No major methodological concerns: Berrington de Gonzalez et al. 2016, Nikkilä et al. 2018, Journy et al. 2015/2016 | Methods: All studies are subject to reverse causation but lagging of doses done to minimize this in all studies, as well as revision of medical records in Berrington de Gonzalez et al. 2016. | Magnitude of effect: The 3 studies demonstrated little to no change in effect when excluding children with leukemia CPFs compared with overall cancer risk suggesting no major effect modification. | Inadequate | The numbers of subjects with Leukemia CPFs in all papers are small; hence, we cannot rule out the existence of an effect. |
Consistency: One study (Journy et al.) found a reduction in HR when excluding subjects with a leukemia CPF; the confidence intervals are wide and numbers are very small so results are consistent with no effect of exclusion (Berrington de Gonzalez et al. 2016) and no heterogeneity (Nikkilä et al. 2018). | Dose response: No evidence of a dose response in subjects with leukemia CPF (Journy et al. 2016). No effect modification (Nikkilä et al. 2018) | ||||
Relevance: All studies were relevant to the PECO question. | |||||
Data adequacy: Statistical power of concern in 1/3 studies (Journy et al. 2015/2016) due to small sample size and short follow-up. Based on the nature of the data, i.e., different CPF considered in different studies and small numbers, risks for individual CPFs cannot be studied. | |||||
Type of effect estimated: Only one study provided an estimate of leukemia risk/dose level in children with leukemia CPFs (Journy et al. 2016). | |||||
Lymphoma | |||||
No indication of a greater lymphoma risk following low–moderate IR exposure, between individuals with a lymphoma CPF and without. | No major methodological concerns: Journy et al. 2015 | Methods: The study is subject to possible confounding by indication and reverse causation but lagging of doses done to minimize this. | Magnitude of effect: ERR/mGy increased slightly when excluding subjects with lymphoma CPFs, suggesting possible slightly lower risk/mGy | Inadequate | The number of subjects with lymphoma CPFs in the study is small; hence, we cannot conclude the existence or not of an effect. |
Consistency: Not applicable considering only one study met criteria. | Dose response: Not reported for lymphoma CPFs | ||||
Relevance: Study relevant to PECO criteria. | |||||
Data adequacy: Statistical power of concern due to small sample size and short follow-up. | |||||
Type of effect estimated: ERR/mGy for lymphoma overall and excluding subjects with lymphoma CPFs. | |||||
Brain and CNS tumor | |||||
No indication of a greater brain and CNS cancer risk following low–moderate IR exposure, between individuals with a brain and CNS tumors CPF vs. without. | No methodological concerns: Berrington de Gonzalez et al. 2016, Meulepas et al. 2019 | Methods: 1/3 studies presented some methodological concerns: short follow-up time and small sample size (Journy et al. 2015/2016). | Magnitude of effect: Studies showed little to no modification of radiation-related brain tumor risk | Inadequate | The numbers of subjects with brain and CNS tumors CPFs in all papers are small; hence, we cannot rule out the existence of an effect. |
Some methodological concerns: Journy et al. 2015/2016 | Consistency: No evidence for heterogeneity (Meulepas et al. 2019), possibly slightly lower risk (Berrington de Gonzalez et al. 2016, Meulepas et al. 2019). | Dose response: No evidence of a dose-response in subjects with brain and CNS tumors CPFs (Journy et al. 2016). No effect modification (Meulepas et al. 2019) | |||
Relevance: All studies were relevant to the PECO question | |||||
Data adequacy: Statistical power of concern in 1/3 studies (Journy et al. 2015/2016) due to small sample size and short follow-up. Based on the nature of the data, i.e., various CPFs considered in different studies and small numbers, risks for individual CPFs cannot be studied. | |||||
Type of effect estimated: Only one study provided an estimate for brain and CNS tumor risk/dose level in children with brain and CNS tumor CPFs (Journy et al. 2016)—the other two compared risk estimates overall with those obtained when excluding subjects with brain and CNS tumor CPFs (Berrington de Gonzalez et al. 2016; Meulepas et al. 2019) | |||||
Breast cancer | |||||
BRCA 1 and 2 | |||||
Most studies suggest a greater risk following very low IR doses among carriers of a BRCA 1 or 2 mutation | No major methodologic concerns: Andrieu et al. 2016, Esther et al. 2007, Esther et al. 2013, Lecarpentier et al. 2011, Pijpe et al. 2012, Little et al. 2014 | Methods: Information about X-rays was collected by questionnaire and is therefore subject to recall bias in all studies. Any effect is expected to be small however. Two studies (Esther et al. 2007, Gronwald et al. 2008) did not adjust for important breast cancer risk factors. | Magnitude of effect: Apart from the studies by Esther and collaborators, the studies of BRCA 1 or 2 mutation carriers show risk estimates for X-rays that are considerably larger than those expected in the general population at these levels of doses. | Limited | Several studies suggest that carriers of mutations in BRCA 1 or 2 have a greater risk of breast cancer following low doses but the results are not consistent across studies and methodological limitations cannot be ruled out. |
Some methodological concerns: Esther et al. 2007, Gronwald et al. 2008 | Consistency: Results of the studies are not entirely consistent. | Dose response: Risk appears to increase with the number of X-rays in Andrieu et al. 2016, Pijpe et al. 2012, and Gronwald et al. 2008. | |||
Relevance: The studies of BRCA1/2: analyses by the number of X-rays not specific to children, except in Pijpe et al. | |||||
Data adequacy: Statistical power of concern in Esther et al. 2013. Concerns regarding validation of exposures with medical records (Andrieu et al. 2016, Lecarpentier et al. 2011, Little et al. 2014). Age at exposures not precise in three studies (Andrieu et al. 2016, Lecarpentier et al. 2011, Gronwald et al. 2008). | |||||
Type of effect estimated: HR or OR by number of X-rays in all studies except Pijpe et al. 2012, where HR was by the level of dose. | |||||
Retinoblastoma | |||||
Reduced risk among those with heritable RB | Some methodologic concern: Kleinerman et al. 2005, Little et al. 2014 | Methods: Both studies did not collect information on important breast cancer risk factors in particular parity. Possible ascertainment bias in Kleinerman et al. 2005 and in the US part of Little et al. 2014. | Magnitude of effect: Heterogeneity of risk between those with heritable and nonheritable RB (Little et al. 2014) | Inadequate | Reduced risk in Little et al. 2014, Kleinerman is not informative. |
Consistency: One study of RB found significant heterogeneity, with no or even a reduced risk of breast cancer in relation to radiation (Little et al. 2014), whereas the other found no effect. | Dose response: No dose-related risk of BC among those with heritable RB | ||||
Relevance: Kleinerman et al. 2005 did not show analyses by the level of radiation dose. | |||||
Data adequacy: Low statistical power in Kleinerman et al. 2005 | |||||
Type of effect estimated: OR at 100 mGy in Little et al. | |||||
Thyroid cancer | |||||
No apparent interaction between CPFs and radiation dose on risk of thyroid cancer | No methodological concerns: Damiola et al. 2014 | Methods: Possible ascertainment and surveillance bias in Momani et al. 2004 | Magnitude of effect: Not reported | Inadequate | Neither study shows an increased risk in those with CPFs but numbers of subjects limited. |
Some methodologic concerns: | Consistency: Both studies are consistent in showing no effect. | Dose-response: Not reported | |||
Momani et al. 2004 | Relevance: All studies considered to meet PECO criteria. | ||||
Data adequacy: Overall data are considered good quality. Statistical power of concern in both studies (Momani et al. 2004, Damiola et al. 2014) is insufficient to be able to detect the subtle variations in susceptibility. | |||||
Type of effect estimated: Only one study provided a measure of risk/dose level (Damiola et al. 2014). | |||||
Level | Definition | ||||
Sufficient | A causal association between the exposure and the outcome has been established. That is, a positive association has been observed in the body of evidence on radiation dose and the outcome of interest in studies in which chance, bias, and confounding were ruled out with reasonable confidence. | ||||
Limited | A causal interpretation of the positive association observed in the body of evidence on radiation exposure and the outcome of interest is credible, but chance, bias, or confounding could not be ruled out with reasonable confidence. | ||||
Inadequate | The available studies are of insufficient quality, consistency, or statistical precision to permit a conclusion to be drawn about the presence or the absence of a causal association between exposure and outcome. | ||||
Evidence of lack of effect | There are several high-quality studies covering the full range of levels of exposure of interest, which are mutually consistent in not showing a positive association between dose/exposure and the studied outcome at any observed level of dose. |
. | . | Elements for assessing the certainty in the evidence . | . | . | |
---|---|---|---|---|---|
Summary of review finding . | Studies contributing to the review finding . | Factors that decrease confidence . | Factors that increase confidence . | CERQual assessment of confidence in the evidence . | Explanation of CERQual assessment . |
Leukemia | |||||
No indication of a greater leukemia risk following low–moderate IR exposure, between individuals with a leukemia CPF and without. | No major methodological concerns: Berrington de Gonzalez et al. 2016, Nikkilä et al. 2018, Journy et al. 2015/2016 | Methods: All studies are subject to reverse causation but lagging of doses done to minimize this in all studies, as well as revision of medical records in Berrington de Gonzalez et al. 2016. | Magnitude of effect: The 3 studies demonstrated little to no change in effect when excluding children with leukemia CPFs compared with overall cancer risk suggesting no major effect modification. | Inadequate | The numbers of subjects with Leukemia CPFs in all papers are small; hence, we cannot rule out the existence of an effect. |
Consistency: One study (Journy et al.) found a reduction in HR when excluding subjects with a leukemia CPF; the confidence intervals are wide and numbers are very small so results are consistent with no effect of exclusion (Berrington de Gonzalez et al. 2016) and no heterogeneity (Nikkilä et al. 2018). | Dose response: No evidence of a dose response in subjects with leukemia CPF (Journy et al. 2016). No effect modification (Nikkilä et al. 2018) | ||||
Relevance: All studies were relevant to the PECO question. | |||||
Data adequacy: Statistical power of concern in 1/3 studies (Journy et al. 2015/2016) due to small sample size and short follow-up. Based on the nature of the data, i.e., different CPF considered in different studies and small numbers, risks for individual CPFs cannot be studied. | |||||
Type of effect estimated: Only one study provided an estimate of leukemia risk/dose level in children with leukemia CPFs (Journy et al. 2016). | |||||
Lymphoma | |||||
No indication of a greater lymphoma risk following low–moderate IR exposure, between individuals with a lymphoma CPF and without. | No major methodological concerns: Journy et al. 2015 | Methods: The study is subject to possible confounding by indication and reverse causation but lagging of doses done to minimize this. | Magnitude of effect: ERR/mGy increased slightly when excluding subjects with lymphoma CPFs, suggesting possible slightly lower risk/mGy | Inadequate | The number of subjects with lymphoma CPFs in the study is small; hence, we cannot conclude the existence or not of an effect. |
Consistency: Not applicable considering only one study met criteria. | Dose response: Not reported for lymphoma CPFs | ||||
Relevance: Study relevant to PECO criteria. | |||||
Data adequacy: Statistical power of concern due to small sample size and short follow-up. | |||||
Type of effect estimated: ERR/mGy for lymphoma overall and excluding subjects with lymphoma CPFs. | |||||
Brain and CNS tumor | |||||
No indication of a greater brain and CNS cancer risk following low–moderate IR exposure, between individuals with a brain and CNS tumors CPF vs. without. | No methodological concerns: Berrington de Gonzalez et al. 2016, Meulepas et al. 2019 | Methods: 1/3 studies presented some methodological concerns: short follow-up time and small sample size (Journy et al. 2015/2016). | Magnitude of effect: Studies showed little to no modification of radiation-related brain tumor risk | Inadequate | The numbers of subjects with brain and CNS tumors CPFs in all papers are small; hence, we cannot rule out the existence of an effect. |
Some methodological concerns: Journy et al. 2015/2016 | Consistency: No evidence for heterogeneity (Meulepas et al. 2019), possibly slightly lower risk (Berrington de Gonzalez et al. 2016, Meulepas et al. 2019). | Dose response: No evidence of a dose-response in subjects with brain and CNS tumors CPFs (Journy et al. 2016). No effect modification (Meulepas et al. 2019) | |||
Relevance: All studies were relevant to the PECO question | |||||
Data adequacy: Statistical power of concern in 1/3 studies (Journy et al. 2015/2016) due to small sample size and short follow-up. Based on the nature of the data, i.e., various CPFs considered in different studies and small numbers, risks for individual CPFs cannot be studied. | |||||
Type of effect estimated: Only one study provided an estimate for brain and CNS tumor risk/dose level in children with brain and CNS tumor CPFs (Journy et al. 2016)—the other two compared risk estimates overall with those obtained when excluding subjects with brain and CNS tumor CPFs (Berrington de Gonzalez et al. 2016; Meulepas et al. 2019) | |||||
Breast cancer | |||||
BRCA 1 and 2 | |||||
Most studies suggest a greater risk following very low IR doses among carriers of a BRCA 1 or 2 mutation | No major methodologic concerns: Andrieu et al. 2016, Esther et al. 2007, Esther et al. 2013, Lecarpentier et al. 2011, Pijpe et al. 2012, Little et al. 2014 | Methods: Information about X-rays was collected by questionnaire and is therefore subject to recall bias in all studies. Any effect is expected to be small however. Two studies (Esther et al. 2007, Gronwald et al. 2008) did not adjust for important breast cancer risk factors. | Magnitude of effect: Apart from the studies by Esther and collaborators, the studies of BRCA 1 or 2 mutation carriers show risk estimates for X-rays that are considerably larger than those expected in the general population at these levels of doses. | Limited | Several studies suggest that carriers of mutations in BRCA 1 or 2 have a greater risk of breast cancer following low doses but the results are not consistent across studies and methodological limitations cannot be ruled out. |
Some methodological concerns: Esther et al. 2007, Gronwald et al. 2008 | Consistency: Results of the studies are not entirely consistent. | Dose response: Risk appears to increase with the number of X-rays in Andrieu et al. 2016, Pijpe et al. 2012, and Gronwald et al. 2008. | |||
Relevance: The studies of BRCA1/2: analyses by the number of X-rays not specific to children, except in Pijpe et al. | |||||
Data adequacy: Statistical power of concern in Esther et al. 2013. Concerns regarding validation of exposures with medical records (Andrieu et al. 2016, Lecarpentier et al. 2011, Little et al. 2014). Age at exposures not precise in three studies (Andrieu et al. 2016, Lecarpentier et al. 2011, Gronwald et al. 2008). | |||||
Type of effect estimated: HR or OR by number of X-rays in all studies except Pijpe et al. 2012, where HR was by the level of dose. | |||||
Retinoblastoma | |||||
Reduced risk among those with heritable RB | Some methodologic concern: Kleinerman et al. 2005, Little et al. 2014 | Methods: Both studies did not collect information on important breast cancer risk factors in particular parity. Possible ascertainment bias in Kleinerman et al. 2005 and in the US part of Little et al. 2014. | Magnitude of effect: Heterogeneity of risk between those with heritable and nonheritable RB (Little et al. 2014) | Inadequate | Reduced risk in Little et al. 2014, Kleinerman is not informative. |
Consistency: One study of RB found significant heterogeneity, with no or even a reduced risk of breast cancer in relation to radiation (Little et al. 2014), whereas the other found no effect. | Dose response: No dose-related risk of BC among those with heritable RB | ||||
Relevance: Kleinerman et al. 2005 did not show analyses by the level of radiation dose. | |||||
Data adequacy: Low statistical power in Kleinerman et al. 2005 | |||||
Type of effect estimated: OR at 100 mGy in Little et al. | |||||
Thyroid cancer | |||||
No apparent interaction between CPFs and radiation dose on risk of thyroid cancer | No methodological concerns: Damiola et al. 2014 | Methods: Possible ascertainment and surveillance bias in Momani et al. 2004 | Magnitude of effect: Not reported | Inadequate | Neither study shows an increased risk in those with CPFs but numbers of subjects limited. |
Some methodologic concerns: | Consistency: Both studies are consistent in showing no effect. | Dose-response: Not reported | |||
Momani et al. 2004 | Relevance: All studies considered to meet PECO criteria. | ||||
Data adequacy: Overall data are considered good quality. Statistical power of concern in both studies (Momani et al. 2004, Damiola et al. 2014) is insufficient to be able to detect the subtle variations in susceptibility. | |||||
Type of effect estimated: Only one study provided a measure of risk/dose level (Damiola et al. 2014). | |||||
Level | Definition | ||||
Sufficient | A causal association between the exposure and the outcome has been established. That is, a positive association has been observed in the body of evidence on radiation dose and the outcome of interest in studies in which chance, bias, and confounding were ruled out with reasonable confidence. | ||||
Limited | A causal interpretation of the positive association observed in the body of evidence on radiation exposure and the outcome of interest is credible, but chance, bias, or confounding could not be ruled out with reasonable confidence. | ||||
Inadequate | The available studies are of insufficient quality, consistency, or statistical precision to permit a conclusion to be drawn about the presence or the absence of a causal association between exposure and outcome. | ||||
Evidence of lack of effect | There are several high-quality studies covering the full range of levels of exposure of interest, which are mutually consistent in not showing a positive association between dose/exposure and the studied outcome at any observed level of dose. |
Leukemia
Three studies considered leukemia risk (refs. 21, 22, 36, 37; Table 2): two cohorts of young CT patients in France (Journy and colleagues; refs. 21, 36) and the UK (Berrington de Gonzalez and colleagues; ref. 22) and one population-based case–control study in Finland (Nikkilä and colleagues; ref. 37). In all studies, historical records of CT examinations were obtained from the Radiological Information Systems of the participating hospitals to reconstruct CT exposure history of the patients. Red bone marrow (RBM) dose estimates were estimated for each CT scan from typical protocols, based on age, gender, examination type, and body part scanned (38–40) independently of disease status. Individual cumulative RBM doses were generally well below 100 mGy, ranging between 2 and 51 mGy. Doses from other medical procedures (e.g., conventional radiography, nuclear medicine, fluoroscopy) were not available. This is unlikely to lead to severe bias, however, as CT is likely to be, by far, the dominant radiation source overall.
Despite the large sample sizes of the studies, the number of leukemia cases among exposed individuals was relatively small: 17 and 74 cases, respectively, in the Journy and colleagues (21, 36) and Berrington de Gonzalez and colleagues (22) studies, and 15 cases in the Nikkilä and colleagues (37) study (Table 3).
Leukemia CPFs considered varied between studies (Table 2): only Down syndrome in the Nikkilä and colleagues study (37); a large number of genetic conditions and immunodeficiencies were considered in Journy and colleagues and Berrington de Gonzalez and colleagues studies (21, 22, 36). The number of exposed cases with leukemia CPFs was small in all studies: 7 and 5, respectively, in Journy and colleagues (21, 36), Berrington de Gonzalez and colleagues and Nikkilä and colleagues (37).
Studies by Journy and colleagues (21, 36) and Berrington de Gonzalez and colleagues (22) assessed the effect of leukemia CPFs on radiation risk estimates by comparing excess relative risk (ERR) estimates including and excluding subjects with these CPFs. Resulting estimates in Berrington de Gonzalez and colleagues (22) were nearly identical (Table 3). In Journy and colleagues (21), the ERR/mGy was higher (0.187 vs. 0.057) in subjects with no CPFs, although findings were based on small numbers of cases and confidence intervals overlapped. In a further analysis of this study (36), the Hazard Ratio among subjects without CPFs was also higher than among those with CPFs, but there was no evidence for heterogeneity of risk between the two groups. There was also no evidence for heterogeneity of risk between subjects with and without Down syndrome in the Nikkilä and colleagues study (ref. 37; risk estimates not shown).
All studies adjusted for the main confounding factors—age, sex, time period and, in by Nikkilä and colleagues (37) and Berrington de Gonzalez and colleagues (22), a measure of socioeconomic status (Table 3). Estimates of RIC in CT studies are subject to potential confounding by indication (meaning children who undergo CT scanning may be at higher risk of cancer due to some underlying condition) and reverse causation (whereby the CT scan was performed to investigate early symptoms of a later diagnosed cancer). Reverse causation is expected to be minimal for leukemia, as CT scanning is generally not used for the initial diagnosis. Reasons for scans could not generally be identified, and thus it is difficult to directly assess the effects of reverse causation. To minimize potential confounding by reverse causation, and to allow for a minimal latency period between exposure and leukemia diagnosis, all studies excluded cancers within 2 years after exposure and lagged doses by 2 years, as is typically done in radiation epidemiology studies (1). Confounding by indication is mainly suspected if subjects with a CPF are more likely than others to undergo CT scanning. As the objective of the analyses presented here is precisely to evaluate whether such predisposing factors might modify the association between radiation dose and cancer risk, confounding by indication is not a concern here.
Overall, we found no evidence that the risk of leukemia following exposure to low–moderate doses of IR differed between children with and without a leukemia CPF (Table 4). Given the small numbers of subjects in the studies, however, we cannot rule out the existence of an effect and conclude there is inadequate evidence of increased risk of radiation-induced leukemia among children with leukemia-related CPFs.
Lymphoma
One study investigated the risk of lymphoma, the Journy and colleagues cohort of pediatric CT patients in France (21). Characteristics of the study are shown in Table 2, and issues of follow-up, dosimetry, and potential biases are described above in the section on leukemia. Nineteen cases of lymphoma were identified (Table 3), seven with a lymphoma CPF. The ERR was slightly lower but statistically compatible with the ERR among subjects without a lymphoma CPF.
We, therefore, found no evidence that the risk of lymphoma following exposure to low-moderate doses of IR was different between children with and without a lymphoma CPF (Table 4). Again, given the small numbers of subjects in the studies, we cannot rule out the existence of an effect and conclude there is inadequate evidence of increased risk of radiation-induced lymphoma among children with a lymphoma-related CPF.
Brain and central nervous system (CNS) tumors
Three studies investigated the risk of brain and CNS tumors (refs. 21, 22, 41; Table 2). Studies by Journy and colleagues (21, 36) and Berrington de Gonzalez and colleagues (22) are described above (section “Leukemia”) with brain doses estimated following the same strategy as RBM dose estimation. The third was a cohort of young CT patients in the Netherlands, Meulepas and colleagues (41)), focusing on tuberous sclerosis complex (TSC). In Berrington de Gonzalez and colleagues (22), 135 brain and CNS tumors were identified, 19 with a brain CPF. In Journy and colleagues, there were 15 cases, 7 with a brain CPF (21, 36), whereas there were 84 cases, with 3 cases of TSC in Meulepas and colleagues (41).
The approach for dose estimation in Meulepas and colleagues (41) was similar to that used in Journy and colleagues (21, 36) and Berrington de Gonzalez and colleagues (22). Brain doses from CT examinations tended to be greater than RBM doses ranging from 1.5 to 77 mGy, with the highest estimated dose in the UK (22) for a single individual being over 300 mGy. Analyses were adjusted for age, sex, calendar period and, in Berrington de Gonzalez and colleagues (22) and Meulepas and colleagues (41), a measure of SES. All studies excluded cases developing cancer within 5 years after exposure and lagged doses by 5 years to minimize potential confounding by reverse causation, a choice supported by results of an independent, population-based series of 695 brain tumor cases ages 10 to 24 years from 14 countries (42).
All three studies assessed the effect of brain CPFs on RIC by comparing ERR/mGy in analyses including and excluding subjects with brain/CNS CPFs. Risk estimates changed minimally when subjects with brain CPFs were excluded (Table 3). In a further analysis of the Journy and colleagues study (36), the hazard ratio among subjects without CPFs was slightly higher than those with CPFs, but there was no evidence for heterogeneity of risk between the two groups.
Overall, we found no evidence that the risk of brain and CNS tumors following exposure at low-moderate doses of IR was different between children with and without CNS CPFs (Table 4). Again, given the small numbers of subjects in the studies, we cannot rule out the existence of an effect and conclude there is inadequate evidence of increased risk of radiation-induced brain and CNS tumors among young people with CPFs.
Breast cancer
BRCA 1 and 2 mutations
Most of the studies identified overlap partly (Table 2). The study by Andrieu and colleagues (43), based on the International BRCA1/2 Carrier Cohort Study (IBCCS), included 1,601 women with a mutation in one of the two genes recruited in Europe and Canada between 1997 and 2002. The study by Lecarpentier and colleagues (46) recruited 1,337 mutation carriers in France between 2000 and 2010, 319 of which were also included in the IBCCS. The cohort study by Pijpe and colleagues (44) included 1,994 mutation carriers, recruited as part of the GENE-RAD-RISK project between 2006 and 2009 in France (including 477 subjects from Lecarpentier and colleagues; ref. 46), the Netherlands, and the UK. There was also overlap between the cohorts in the Esther and colleagues (48) and Esther and colleagues (45) nested case–control studies. The former included 402 cases with a family history of breast cancer, whereas 271 BRCA1/2 mutation carriers were considered in the latter (Table 2). Only the Polish case-case study of Gronwald and colleagues (47), is entirely independent. All studies of BRCA1/2 mutation carriers were based on cohorts assembled from family cancer clinics, except Gronwald and colleagues (47), who recruited early onset of breast cancer cases from treatment centers across Poland and tested them for BRCA 1 mutations. Recruitment from family clinics may induce a surveillance bias as disease status can affect the likelihood of case ascertainment, leading to an oversampling of affected women (Supplementary Table S3); however, analyses were corrected for such a bias through appropriate methods (51, 52).
Survival is another potential source of bias in studies of BRCA 1 and 2 mutation carriers, where prevalent cases may have answered the questionnaire years after diagnosis of their diseases, whereas other cases died from breast cancer before questionnaire completion (Supplementary Table S3). The studies by Andrieu and colleagues (43), Pijpe and colleagues (44), and Esther and colleagues 2013 (45) presented analyses of subcohorts of “pseudo-incident” or relatively recent cases (diagnosed or censored within the five years before completion of the questionnaire) to minimize this potential bias.
A total of 126 exposed cases were identified in the “pseudo-incident” cohort of 969 carriers of BRCA1/2 mutations in Andrieu et al, 365 in the subcohort of 990 women in Lecarpentier and colleagues and 49 in the subcohort of 1122 women in Pijpe and colleagues (Table 3). The number of exposed cases was not specified in Gronwald and colleagues (47). In the case–control study by Esther and colleagues (48), out of 402 cases with a family history of breast cancer, 25 reported at least one X-ray for TB or pneumonia and 305 at least one chest X-ray for other purposes. In the Esther and colleagues study (45), 36 out of 167 cases with a BRCA1 mutation and 24 out of 104 cases with a BRCA2 mutation reported at least one chest X-ray.
No individual dose reconstruction was conducted in most of the studies of BRCA1/2 mutation carriers. Instead, breast cancer risk was evaluated as a function of the number of chest X-rays received (Table 2). No distinction was made between fluoroscopy and conventional X-rays, the former resulting in doses about 10 times higher, on average, during the time period of relevance for exposures in these cohorts than the latter (44). Individual cumulative doses were estimated only in the study of Pijpe and colleagues (44), considering the types of examinations the subjects had undergone and nominal estimates of breast dose for fluoroscopy, radiography, mammography, and CT derived from a literature review and expert judgment—the average dose was low (14 mGy), of the order of several years of annual natural background radiation. Information on diagnostic procedures, collected through a questionnaire in studies of BRCA 1/2 mutation carriers, is subject to recall bias (Supplementary Table S3). The extent of this bias is likely to be small, however, in populations of women who are at high breast cancer risk due to the mutations they carry.
All studies of BRCA 1/2 mutation carriers adjusted for the main breast cancer risk factors, except Esther and colleagues (48), which did not adjust for parity, and Gronwald and colleagues (47), where adjustment variables are not clear.
The Andrieu and colleagues study (43) reported hazard ratios (HR) that increased with an increasing number of X-rays, with the highest risk estimate among subjects who only reported exposures before age 20 (Table 3). This pattern of risk, similar to that seen in other populations exposed to radiation at considerably higher levels, suggests that women with BRCA1 or 2 mutations might be at higher risk of radiation-induced breast cancer than the general population. A similar pattern was seen in Gronwald and colleagues (47), where risk in carriers compared with noncarriers increased with the number of X-rays. The Pijpe and colleagues study (44), with individual dose estimates, also suggests an increased risk at very low doses, much lower than those at which similar increases have been observed in the general population. In Lecarpentier and colleagues (46), though elevated HRs were observed in all categories of numbers of X-rays, no trend with the number of X-rays was found, and the HR was somewhat higher (but statistically compatible) in subjects who reported a later age at first exposure.
Esther and colleagues (48), however, provide no evidence of differential risk between those with and without a family history of beast cancer, whereas Esther and colleagues (45), based on small numbers of exposed cases found no trend in OR by number of X-rays and no greater risk in those youngest at exposure.
The differences in results between the studies are difficult to interpret. Although the studies by Andrieu and colleagues (43), Gronwald and colleagues (47), Pijpe and colleagues (44), and Lecarpentier and colleagues (46) all found increased risks of breast cancer following extremely low doses (typically equivalent to a few days of natural background radiation), the two case–control studies by Esther and collaborators (45, 48) did not. The number of cases studied by Esther and colleagues (45) was low, however, and the proportion of subjects reporting chest X-rays is substantially lower than in the other studies. Further, although analyses of risk by age at first exposure (below 20 years and 20 and above) were conducted in all BRCA1/2 mutation carrier studies, the analyses by the number of X-rays are not stratified by age at exposure, and hence direct estimation of the effect of BRCA 1/2 mutations on breast cancer risk from exposure to radiation below the age of 20 is possible only in one study, that of Pijpe and colleagues (44). Differences in the distribution of X-rays at different ages may therefore confound a possible association.
Overall, therefore, we found limited evidence that carriers with a BRCA1/2 mutation might modify breast cancer risk following exposure before the age of 20 (Table 4). Most studies suggest an increased risk but the results are not consistent across studies and methodological limitations cannot be ruled out.
Retinoblastoma
Kleinerman and colleagues (49) studied breast cancer risk in a cohort of women who had survived retinoblastoma from two US hospitals. Little and colleagues (50) conducted a case–control study of breast cancer, nested within the Kleinerman cohort (49) and within a separate UK cohort of childhood retinoblastoma survivors (Table 2).
In Kleinerman and colleagues (49), information was available only on whether subjects underwent radiotherapy or not, with no indication of the dose. In their case–control study, however, Little and colleagues (50) estimated individual doses for each subject, based on RT treatment details from records and detailed modeling of scatter radiation to the breast tissue based on doses to the eye.
In Kleinerman and colleagues (49), breast cancer cases were ascertained through contact with the study subjects or their families; hence, it is possible that some cases were missed (Supplementary Table S3). Diagnoses were validated with hospital records. As the US subjects in Little and colleagues (50) come from the Kleinerman and colleagues (49) study, the same limitation applies; the UK cases, however, were identified through linkage with the national population-based cancer registry. A further limitation of both studies is the lack of adjustment for important breast cancer risk factors, in particular parity (Supplementary Table S3).
Kleinerman and colleagues (49) found a similar SIR for breast cancer among those who were exposed to radiation and those who were not, but results are based on very small numbers of exposed cases (Table 3). Little and colleagues (50) found heterogeneity in risk between those with hereditary and nonhereditary retinoblastoma, with a lower OR among those with hereditary retinoblastoma. There was evidence of a dose–response relationship only among those whose retinoblastoma was not hereditary.
Overall, we found “inadequate” evidence supporting a reduced risk of radiation-induced breast cancer among individuals with hereditary retinoblastoma, based only on one study (50).
Thyroid cancer
Two studies investigated the risk of thyroid cancer (refs. 53, 54; Table 2). The first study, by Damiola and colleagues (53), studied polymorphic associated thyroid carcinoma (TC) risk following the Chernobyl nuclear accident in a population-based case–control study, of 83 cases and 324 controls from Belarus exposed in childhood. The second study, by Momani and colleagues (54), investigated familial concordance in relation to thyroid cancer risk in 251 exposed sibling pairs, with 83 thyroid cancer cases, from a cohort of 4,296 children treated for benign (head/neck) disorders in the USA.
In Damiola and colleagues (53), individual doses were estimated for each study subject (55) taking into account information obtained by questionnaire on the whereabouts and dietary habits of the study subjects and information on environmental contamination available for each settlement where they resided. The main contribution to thyroid dose was from 131I, predominantly from milk intake. In Momani and colleagues (54), individual doses were estimated from RT records and modeling (56).
Case ascertainment is expected to be reasonably complete in Damiola and colleagues (53), where cancer cases were found in all appropriate medical and surgical departments in the study regions. In Momani and colleagues (54), however, cases were ascertained by questionnaires addressed to cohort members. There is, therefore, potential for ascertainment bias, as well as surveillance bias as most members of the cohort were under surveillance for follow-up of their head/neck disorder.
Damiola and colleagues (53) found associations between papillary TC and a number of single-nucleotide polymorphism (SNP) and genes, as well as between papillary TC and radiation dose. Overall, however, the genetic polymorphisms studied and radiation dose appeared to act independently on the risk of TC, though the numbers of subjects in each analysis were small (Table 3). In Momani and colleagues (54), familial influence on TC risk was assessed by evaluating whether the distribution of tumors within family pairs could be accounted by known risk factors, considering years at risk, sex, age at exposure, and radiation dose. The distribution of concordant and discordant pairs was as expected (Table 3), suggesting no familial influence on the risk of radiation-induced thyroid cancer.
Overall, we found “inadequate evidence” that the risk of radiation-induced thyroid cancer following exposure to IR at low–moderate doses was different between children with and without a thyroid CPF. Neither study found an increased risk among those with CPFs but the numbers of subjects in the analyses limit the statistical power of the studies.
Discussion
The current systematic review aimed to identify cancer-predisposing factors that may modify the risk of radiation-induced cancer following exposure to low–moderate doses of ionizing radiation in populations below 25 years of age. To our knowledge, this is the first systematic review on the topic. Based on the scientific literature reviewed, we concluded that there was inadequate evidence that such factors increase or decrease the risk of radiation-induced leukemia, lymphoma, brain/CNS tumors, and thyroid cancers. However, for breast cancer, we found limited evidence that BRCA1/2 mutation might increase the risk following very low doses of radiation, and this needs to be further explored.
Most of the studies reviewed—even the large-scale cohort studies of pediatric CT patients—identified very small numbers of exposed subjects with CPFs, resulting in low statistical power, as seen also in a recent systematic review of genetic susceptibility to radiation-induced thyroid cancer (57). The low prevalence of CPFs in these studies is not surprising considering the rarity of these conditions in the general population as well as, for some CPFs, the short life expectancy of the carriers (8, 23, 58). The CPF with highest prevalence in these studies was organ transplantation (about 1% in the Journy and colleagues study; ref. 21), a known risk factor for lymphoma (59). Prevalence was lower for other CPFs, 0.3% for Down syndrome and 0.05% for TSC in Journy and colleagues (21) and Meulepas and colleagues (41), respectively, and much less than 1/1,000 or 1/10,000 for most of the others (22). Because of this, analyses by Journy and colleagues (21, 36) and Berrington de Gonzalez and colleagues (22) have considered all CPFs as a group, when in fact any susceptibility conferred is likely to vary between types, and even these analyses were underpowered as shown above.
The only studies with some statistical power to evaluate a possible effect were studies of cohorts of women selected on the basis of CPF status—namely, women with a BRCA1 or 2 mutation or with hereditary RB—and hence all, or the majority of study participants, had a CPF. Several of these studies suggest that risk among BRCA1/2 carriers at very low doses (of the level of one-year dose from natural background radiation) is much higher than in the general population, increases with the number of chest X-rays and may be highest for exposures at younger ages. Results are not entirely consistent across studies; hence, they provide only limited evidence of an effect of CPF on risk of RIC. The studies reviewed are subject to some limitations that need to be better addressed in the future. Apart from Pijpe and colleagues, studies (43, 46, 47) only considered chest X-ray exposures; whereas in these studies, X-rays (both fluoroscopy and conventional) were likely to be the main source of dose, missing doses from other procedures may potentially bias risk estimates. Moreover, Pijpe and colleagues (44) was the only study providing individual radiation dose estimates to the breast. Information about exact age at exposure was generally missing, with information collected only about X-ray procedures in different age ranges. Though a number of other specific gene mutations were included in our systematic review, only studies of RB and BRCA1/2 mutations were found.
The effect of mutations in a number of specific genes—including BRCA1, BRCA2, ATM, TP53, CHEK2—on risk of radiation-induced breast cancer has only been examined systematically in one study, the large multinational WECARE study of contralateral breast cancer following moderate–high doses of RT in adults (60, 61). WECARE provided no clear indication that carriers of BRCA1/2 mutations were more susceptible to RIC than noncarriers (60), though ATM mutations appeared to increase the risk of contralateral breast cancer after RT (62).
Although the role of specific mutations as modifiers of radiation-induced breast cancer risk in cancer survivors is unclear due to the rarity of these mutations, recent genetic associations studies in adult women who received low doses of radiation in occupational settings suggest that common susceptibility variants may play an important role in modulating RIC (63–69). Indeed, a genome-wide association study identified specific variants that appear to confer a higher risk of radiation-induced breast cancer among childhood cancer survivors (70). Polygenic risk scores (PRS), aggregating associations with many variants, are increasingly being used in gene–environment interaction to identify risks that may not be detectable for individual variants (71). This method was also used in the WECARE study, in which a PRS comprising variants in DNA repair pathways was associated with an increased risk of subsequent radiation-associated contralateral breast cancer (72). Similarly, the use of a PRS among Hodgkin lymphoma patients confirms the existence of genetic susceptibility to radiation-induced breast cancer (73). These results have important implications for the radiation protection of patients.
To adequately address whether CPFs may modify the risk of RICs in young people, further studies with sufficient power are necessary. Given the rarity of CPFs in the general population, informative studies should be based either on (i) cohorts of persons who are carriers of mutations of interest—e.g., BRCA1/2, AT heterozygotes—in which detailed information regarding radiation exposure can be obtained and doses estimated either at the level of the cohort or in a nested case–control study; or (ii) general population/patient studies with adequate individual dosimetry, focusing on common variants using PRS.
Children with genetic conditions that confer an increased susceptibility to RIC are of particular concern in medical radiation protection (8, 11). If such children can be identified, personalized screening, surveillance, management, and treatment can be offered to reduce their risk of RIC. The studies identified in this systematic review are too limited—mainly because of low statistical power—to address this issue, with the exception of studies of women with BRCA1/2 mutations that provided limited evidence of an increased risk of RIC. Further studies with more appropriate study designs are needed to better identify cancer-predisposing conditions/variants that may increase the risk of RIC.
Authors' Disclosures
No disclosures were reported.
Acknowledgments
This work was performed within the MEDIRAD project, which has received funding from the Euratom research and training program 2014–2018 under grant agreement No 755523.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Note: Supplementary data for this article are available at Cancer Epidemiology, Biomarkers & Prevention Online (http://cebp.aacrjournals.org/).