The use of common analgesics, such as aspirin, non-aspirin NSAIDs and acetaminophen, may be associated with the risk of several hormone-related cancers, possibly via effects on sex hormone and prolactin concentrations. Therefore, we assessed the cross-sectional relationship between analgesic (aspirin, non-aspirin NSAIDS, and acetaminophen) use and sex hormone and prolactin concentrations among 2,034 premenopausal women, 32 to 54 years old, from the Nurses' Health Study II. Approximately 84% of women provided timed luteal and follicular blood samples; the remainder provided a single untimed sample. After adjustment for age, BMI, parity, and other potential confounders, acetaminophen use was inversely associated with prolactin levels (ptrend= 0.04). Compared to non-users, prolactin concentrations were 11.8% lower among women who reported using acetaminophen at least 2 times per week (p<0.01). Similarly, acetaminophen was inversely associated with free testosterone, when comparing use at least 2 times per week to non-users (7.1% lower, p = 0.04; ptrend= 0.04). Use of non-aspirin NSAIDs was positively associated with follicular free estradiol, with 13.5% higher levels in women who reported use 4 or more days per week as compared to non-users (p = 0.04; ptrend= 0.11); results for free follicular estradiol were similar (13.2% higher, p = 0.06; ptrend= 0.11) though not statistically significant. There were no clear associations observed between analgesic use and luteal estradiol, estrone, estrone sulfate, DHEA, DHEAS, androstenedione, and estrogen/androgen ratios. After adjustment for multiple comparisons, none of the associations were statistically significant. In conclusion, our study provides modest evidence for an association of acetaminophen use with prolactin and free testosterone concentrations in premenopausal women, and the suggestion of an association between non-aspirin NSAIDs and follicular free estradiol among those with frequent use.

Citation Format: Scott R. Bauer, Renee T. Fortner, Margaret A. Gates, Heather Eliassen, Susan E. Hankinson, Shelley S. Tworoger. Analgesic use in relation to sex hormone and prolactin concentrations in premenopausal women. [abstract]. In: Proceedings of the AACR Special Conference on Post-GWAS Horizons in Molecular Epidemiology: Digging Deeper into the Environment; 2012 Nov 11-14; Hollywood, FL. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2012;21(11 Suppl):Abstract nr 77.