Effects of indol-3-carbinol(I3C) on tight junction activity in prostate cancer cells- LNCAP, PC3 and DU145.

B115

To determine whether I3C inhibits the cell motility, progression and metastasis of prostate cancer cells, this experiment was performed. Indole-3-carbinol (I3C) is a compound found in cruciferous vegetables such as cabbage, cauliflower, broccoli and Brussels sprouts. Our results indicate that I3C inhibit cell growth of LNCAP, PC3 cells in a dose(50uM, 100uM) and time(24, 48 and 72 hrs) dependent manner. Using the wound healing experiment and cell invasion study, I3C inhibits the cell motility of the prostate adenocarcinoma cell line, DU145. The TER values was increased in DU145 cells grown in transwells treated with I3C. Claudin-3 and claudin-4 have been shown to be overexpressed in prostate cancer cells and in particular, in metastatic prostate cancer cells. But I3C occurs concurrently with a significant decrease in the levels of the proteins, claudin-3 and claudin-4 in prostate cancer cells but those of PC3 were not significantly different. This result suggests that I3C may be expected to inhibit cancer progression and metastasis by restoring tight junction and their composite protein in prostate cancer.