Endometrial cancer is a component of hereditary nonpolyposis colorectal carcinoma, primarily the consequence of mutations in genes involved in mismatch repair (MMR; MSH2, MLH1, PMS1, and PMS2). In addition to the repair of DNA replication errors, MMR genes have been implicated in homologous recombination repair (HRR) in yeast and in mammalian cells (1, 2). The involvement of the MMR genes in the DNA HRR pathway implies that defects in the HRR pathway may contribute to the development of hereditary nonpolyposis colorectal carcinoma tumorigenesis. Recently, Mohindra et al. (3) reported that human tumor cell lines (colon, uterine, ovarian, and endometrial) deficient in MMR also had defects in HRR induced by DNA double-strand breaks, and these tumor cell lines were hypersensitive to thymidine, which was not due to MMR deficiency or deoxynucleoside triphosphate pool imbalance. A XRCC2 frameshift mutation identified in the MMR-deficient uterine tumor cell line SKUT-1 conferred hypersensitivity to thymidine or mitomycin C in a MMR-proficient line, suggesting that HRR defects may lead to the thymidine sensitivity of MMR-deficient tumor cell lines (3). These findings additionally support the hypothesis that HRR deficiency may confer susceptibility to hereditary nonpolyposis colorectal carcinoma tumorigenesis. XRCC2 and XRCC3, as two RAD51 paralogues, facilitate the formation of RAD51 foci formation in HRR. We assessed whether candidate polymorphisms in XRCC2 and XRCC3 genes in HRR pathway are associated with endometrial cancer risk in a nested case-control study within the Nurses’ Health Study.

This nested case-control study (cases, n = 220; controls, n = 666) included both prevalent and incident pathologically confirmed endometrial cancer cases diagnosed up to June 1, 1998, from the blood subcohort of the Nurses’ Health Study. Controls were matched to cases (3:1) on year of birth, menopausal status at blood draw, and hormone replacement therapy status at blood draw. The characteristics of cases and controls have been described previously (4). Genotyping assays were performed by the 5′-nuclease assay (TaqMan) using the ABI Prism 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA). Genotyping was performed by laboratory personnel blinded to case-control status, and blinded quality control samples were inserted to validate genotyping procedures; concordance for the blinded samples was 100%. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using conditional logistic regression. We inferred haplotypes using the ARLEQUIN 2.0 software package (5).

We observed no overall associations of the four genotypes with endometrial cancer risk (Tables 1 and 2). We observed that, compared with the XRCC2 31479 G/G genotype, the multivariate ORs for the G/A and A/A genotypes were 0.95 (95% CI, 0.58–1.54) and 2.05 (95% CI, 0.24–17.82), respectively. Three common haplotypes in XRCC3 accounted for 99% of chromosomes in the present study population. As compared with women who were homozygous for XRCC3 4541A, women who were heterozygous for 4541G allele had a multivariate risk of 1.00 (95% CI, 0.69–1.43), and women who were homozygous for 4541G allele had a multivariate risk of 0.75 (95% CI, 0.30–1.87). As compared with noncarriers, the multivariate ORs for women with one XRCC3 17893G allele and two alleles were 0.95 (95% CI, 0.66–1.36) and 0.69 (95% CI, 0.39–1.21), respectively, with an OR of 0.89 (95% CI, 0.63–1.25) for carriage of at least one G allele. As compared with women with 18067 C/C genotype, women with C/T and T/T genotypes had multivariate OR of 1.03 (95% CI, 0.72–1.47) and 1.15 (95% CI, 0.67–1.98), respectively. No significant interactions were observed between these polymorphisms and risk factors such as body mass index, weight gain, and smoking (pack-years).

We observed neither associations of XRCC2 and XRCC3 polymorphisms with endometrial cancer risk, nor significant differences in XRCC3 haplotype distribution in cases and controls. The fairly large number of cases, the prospective collection of covariate information, and the high follow-up rates strengthen the validity of this study. Given the sample size and the allele frequencies among controls, we had 95% power for the XRCC2 G31479A and 99% for the three XRCC3 polymorphisms of detecting the OR of 2.0 for the variant allele carriers versus noncarriers. The functional effects of the two polymorphisms XRCC2 G31479A (R188H) and XRCC3 C18067T (T241M) on cell survival after mitomycin C-induced DNA interstrand cross-linking have been studied in in vitro experiments (6, 7). Neither of the two variants displayed a significant effect on damage sensitivity. Genetic variations in XRCC2 and XRCC3 genes have recently been evaluated in relation to breast cancer risk. This is the first article of polymorphisms in HRR and endometrial cancer risk. In summary, we did not provide evidence that women with the polymorphisms in XRCC2 and XRCC3 genes had an altered risk of endometrial cancer.

Grant support: NIH Grant CA82838 and Grant RSG-00-061-04-CCE.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Requests for reprints: Jiali Han, Harvard Center for Cancer Prevention, Harvard School of Public Health, Building II, 665 Huntington Avenue, Boston, MA 02115. E-mail: [email protected]

Table 1

Associations between XRCC2 and XRCC3 genotypes and endometrial cancer risk

CasesControlsMatched odds ratioaMultivariate odds ratiob
XRCC2 G31479A (Arg188His)     
 G/G 183 557 1.00 1.00 
 G/A 32 99 1.03 (0.67–1.60) 0.95 (0.58–1.54) 
 A/A 2 3 2.02 (0.34–12.08) 2.05 (0.24–17.82) 
 Ptrend   0.68 0.97 
 G/A + A/A 34 102 1.06 (0.69–1.63) 0.98 (0.61–1.57) 
XRCC3 A4541G (5′-UTR)     
 A/A 140 438 1.00 1.00 
 A/G 73 200 1.13 (0.82–1.58) 1.00 (0.69–1.43) 
 G/G 7 25 0.85 (0.37–1.98) 0.75 (0.30–1.87) 
 Ptrend   0.74 0.71 
 A/G + G/G 80 225 1.10 (0.80–1.51) 0.97 (0.68–1.37) 
XRCC3 A17893G (IVS5–14)     
 A/A 100 274 1.00 1.00 
 A/G 97 296 0.90 (0.65–1.25) 0.95 (0.66–1.36) 
 G/G 23 89 0.71 (0.42–1.18) 0.69 (0.39–1.21) 
 Ptrend   0.19 0.26 
 A/G + G/G 120 385 0.86 (0.63–1.16) 0.89 (0.63–1.25) 
XRCC3 C18067T (Thr241Met)     
 C/C 94 280 1.00 1.00 
 C/T 97 306 0.96 (0.70–1.33) 1.03 (0.72–1.47) 
 T/T 29 79 1.12 (0.69–1.82) 1.15 (0.67–1.98) 
 Ptrend   0.80 0.64 
 C/T + T/T 126 385 0.99 (0.73–1.35) 1.05 (0.75–1.48) 
CasesControlsMatched odds ratioaMultivariate odds ratiob
XRCC2 G31479A (Arg188His)     
 G/G 183 557 1.00 1.00 
 G/A 32 99 1.03 (0.67–1.60) 0.95 (0.58–1.54) 
 A/A 2 3 2.02 (0.34–12.08) 2.05 (0.24–17.82) 
 Ptrend   0.68 0.97 
 G/A + A/A 34 102 1.06 (0.69–1.63) 0.98 (0.61–1.57) 
XRCC3 A4541G (5′-UTR)     
 A/A 140 438 1.00 1.00 
 A/G 73 200 1.13 (0.82–1.58) 1.00 (0.69–1.43) 
 G/G 7 25 0.85 (0.37–1.98) 0.75 (0.30–1.87) 
 Ptrend   0.74 0.71 
 A/G + G/G 80 225 1.10 (0.80–1.51) 0.97 (0.68–1.37) 
XRCC3 A17893G (IVS5–14)     
 A/A 100 274 1.00 1.00 
 A/G 97 296 0.90 (0.65–1.25) 0.95 (0.66–1.36) 
 G/G 23 89 0.71 (0.42–1.18) 0.69 (0.39–1.21) 
 Ptrend   0.19 0.26 
 A/G + G/G 120 385 0.86 (0.63–1.16) 0.89 (0.63–1.25) 
XRCC3 C18067T (Thr241Met)     
 C/C 94 280 1.00 1.00 
 C/T 97 306 0.96 (0.70–1.33) 1.03 (0.72–1.47) 
 T/T 29 79 1.12 (0.69–1.82) 1.15 (0.67–1.98) 
 Ptrend   0.80 0.64 
 C/T + T/T 126 385 0.99 (0.73–1.35) 1.05 (0.75–1.48) 
View Large
Table 2

XRCC3-inferred haplotypes in cases and controls

A4541GHaplotypes A17893GC18067TAllele frequency (%)P
Cases (n = 440)Controls (n = 1314)
0 0 0 0.13 0.10 0.24 
0 0 1 0.35 0.35 0.94 
0 1 0 0.33 0.36 0.32 
1 0 0 0.20 0.19 0.76 
A4541GHaplotypes A17893GC18067TAllele frequency (%)P
Cases (n = 440)Controls (n = 1314)
0 0 0 0.13 0.10 0.24 
0 0 1 0.35 0.35 0.94 
0 1 0 0.33 0.36 0.32 
1 0 0 0.20 0.19 0.76 
View Large

We thank Dr. Hardeep Ranu, Robert O’Brien, Pati Soule, and Alicia Whittington for their laboratory assistance and Rong Chen for her programming support. We also thank the participants in the Nurses’ Health Study for their dedication and commitment.

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