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cell-cycle

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Journal Articles
Cancer Res (2022) 82 (12_Supplement): 5382.
Published: 15 June 2022
... by PrestoBlue assay. Cell cycle was measured by PI staining and cell voltage potentials were assessed using DiBAC4 staining. It is well-established that cancer cells have distinct bioelectrical properties and most have depolarized cell voltage potentials that support cell proliferation. Expression of cell...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 5414.
Published: 15 June 2022
... in regulating prostate cancer growth. Results: We identified 1814 DEGs after inhibiting CYP3A5 using siRNA pool in MDAPCa2b cells, 825 of which increased or decreased expression greater than two-fold. The differentially down-regulated genes associate with several growth regulatory pathways: cell cycle...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 5374.
Published: 15 June 2022
... NF-κB mediating apoptosis to achieve anti-tumor effects in hepatocellular carcinoma. p53, a tumor suppressor gene that could inhibit cell division and proliferation through regulating with cell cycle and apoptosis related molecules. Patients with p53 gene mutation were often resistant to current...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 5388.
Published: 15 June 2022
... cycle-related genes was reduced at 24h upon treatment with single-agent palbociclib, but rebounded to baseline by 96h. Combination with giredestrant induced the expected downregulation of ER activity at both 24 and 96h. Notably, the combination further reduced the expression of cell cycle-related genes...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 2310.
Published: 15 June 2022
... and methylation of non-histone substrates, the latter modulating protein stability or actin polymerization. Moreover, EZH2 is known to play a role in regulating cell cycle progression. However, it remains unclear whether EZH2 has an enzyme-independent function in the cell cycle. Here we show that EZH2 knockdown...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 2312.
Published: 15 June 2022
... to VGSC expression that varies across the cell cycle. We assessed cell surface expression of VGSCs and Na+,K+-ATPase during phases of the cell cycle in which there are single copies of DNA compared with phases in which DNA has doubled. For this, DNA was labeled with either propidium...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 2313.
Published: 15 June 2022
... to colon cancer cells induced apoptosis within 24 hours, which was extended for a long-term. We revealed that fenbendazole markedly suppressed proliferation rate via cell cycle arrest. Cell cycle progression is elaborately regulated by multiple genes, such as cyclins and cyclin-dependent kinases (CDKs...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 1775.
Published: 15 June 2022
...Nischal Koirala; Jennifer Aske; Xiaoqian Lin; Nandini Dey; Pradip De Background: Proliferative signaling of the HER2- and ER-pathways converge and propagate via cyclin D and CDK4/6, leading to uncontrolled cell cycle progression and resistance to anti- HER2- and ER-targeted therapies. Purpose: We...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 5482.
Published: 15 June 2022
... term, R001-treated TNBC cells showed evidence of pyroptotic cell death. Prolonged treatment of TNBC cells with R001 led to DNA damage and G2/M-phase cell cycle arrest, which are late-stage responses that were preceded by the suppression of ATM-Chk2-Cdc25c pathway, and the induction of p21 and γH2AX...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): LB088.
Published: 15 June 2022
... inhibition enhances HER3-DXd’s antitumor efficacy. The antitumor effects of HER3-DXd plus the synergistic partners were assessed using a soft agar colony formation assay and a clonogenic assay in TMR HER3+/ER+ MCF7 and T47D BC cells. Treatment effects on cell cycle distribution...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 6183.
Published: 15 June 2022
... with an extremely low IC50 (0.1 µM). IP-DNQ evoked massive ROS production and oxidative DNA lesions, resulting in PARP1 hyperactivation and mitochondrial catastrophe. Furthermore, we also observed that IP-DNQ induced G2/M phase cell cycle arrest and promoted programmed necrosis and caspase-dependent apoptosis co...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 1553.
Published: 15 June 2022
... of the interactions between the miRs and their targets led to the selection of the Cdk6 as the target of miR-1 to be further investigated, based on its: 1) upregulation in AOM/DSS-induced dysplasia; and 2) association with cell cycle progression and inflammatory signaling. The goal of the present study...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): CT113.
Published: 15 June 2022
... v4.0 every cycle. CBC was performed on day 8 of cycle 1 for ANC nadir. Pretreatment clinical samples were collected for biomarker analysis including HR deficiency and circulating tumor cells (CTC). Results: Between January 2017 and November 2020, 49 patients (median age 64.1 years [IQR 56.8 - 69.9...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 3982.
Published: 15 June 2022
... transmitted and reduced expression of complex molecules and also effected their association leading to p53 activation. CDDP-activated p53 led to cell cycle arrest and induced both early and late apoptosis in APL cells. Our findings also showed that CDDP stimulated more promyelocytes formation with dense...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 5647.
Published: 15 June 2022
... homolog (PTEN) is a tumor suppressor gene whose mutations have been found to be one of the most frequent events in GBM. PTEN acts as a negative regulator of the phosphoinositide 3-kinase (PI3K) pathway. GBM transcriptome data from The Cancer Genome Atlas (TCGA) revealed a positive enrichment of cell cycle...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 1268.
Published: 15 June 2022
... overexpression of cyclin D1 and/or CDK4/6 has been detected in the majority of sarcomas even when wild-type Rb protein is expressed. Hence, inhibition of cell cycle progression through reactivation of the tumor suppressor function of Rb by inhibiting CDK4/6 activity poses a potential avenue for new therapeutic...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 159.
Published: 15 June 2022
... (TK1) plays an important role in DNA synthesis and repair. Normally, it is highly expressed during S phase and provides recycled nucleotides by phosphorylating thymidine to make thymidine monophosphate. Past studies have suggested that TK1 may influence cell cycle control and, therefore, provides...
Journal Articles
Cancer Res (2022) 82 (4_Supplement): P5-10-06.
Published: 15 February 2022
..., Arginine deprivation can be exploited as a potential targeted therapy for the treatment of various cancers. Arginase, an enzyme of the urea cycle, converts L-arginine to L-ornithine, which is the precursor of polyamines that are essential components of cell proliferation. However, Arginine deprivation...
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ALKBH5 promotes <span class="search-highlight">cell</span>-<span class="search-highlight">cycle</span> progression by regulating <span class="search-highlight">cell</span> <span class="search-highlight">cycle</span> and DNA rep...
Published: 16 May 2022
Figure 2. ALKBH5 promotes cell-cycle progression by regulating cell cycle and DNA replication–associated gene expression. A, Volcano plot showing gene expression changes in 143B cells transfected with scrambled siRNA or siALKBH5 (KD) for 48 hours. Cut-off criteria for differentially expressed genes include absolute log2-fold change >1 and P value < 0.05 (red, differentially increased; blue, decreased genes). B, Results of gene ontology (GO) analysis showing highly enriched biological processes in ALKBH5 KD 143B cells (reduced genes). C, Results of qRT-PCR analysis of target gene expression in 143B cells transfected with scrambled or ALKBH5 siRNA-1 (siALKBH5). D, Western blots of target genes in scrambled and two different siRNAs against ALKBH5 (siALKBH5–1 and siALKBH5–2)-transfected 143B cells using antibodies against the indicated proteins. β-Actin was used as the loading control. Gel photograph is representative of at least three independent experiments. The Western blots showing MCM2, CDC6, CCNB1, WEE1, and β-actin were run on one gel (after stripping), while CDK2 and β-actin were run on a separate gel. E, Cell-cycle distribution of 143B, OS-17, or hFOB cells transfected with scrambled or ALKBH5 siRNA. Propidium Iodide was used to stain for DNA content and flow cytometry analysis was done to identify cell population in different phases of cell cycle using FlowJo software. F, Bivariate cell-cycle analysis of EdU immunofluorescence versus DNA content of U2OS cells pulse labeled with EdU. Bar graph shows quantification of EdU+ cells in scrambled and ALKBH5 siRNA–transfected U2OS cells. P values calculated using standard Student t tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Bar graphs represent means ±SEM for at least three independent experiments. Figure 2. ALKBH5 promotes cell-cycle progression by regulating cell cycle and DNA replication–associated gene expression. A, Volcano plot showing gene expression changes in 143B cells transfected with scrambled-siRNA or siALKBH5 (KD) for 48 hours. Cut-off criteria for differentially expressed genes include absolute log2 fold change >1 and P value < 0.05 (red indicates differentially increased and blue represents decreased genes). B, Results of gene ontology (GO) analysis showing highly enriched biological processes in ALKBH5 KD 143B cells (reduced genes). C, Results of qRT-PCR analysis of target gene expression in 143B cells transfected with scrambled or ALKBH5-siRNA-1 (siALKBH5). D, Western blots of target genes in scrambled and two different siRNAs against ALKBH5 (siALKBH5–1 and siALKBH5–2) transfected 143B cells using antibodies against the indicated proteins. β-Actin was used as the loading control. Gel photograph is representative of at least three independent experiments. The Western blot showing MCM2, CDC6, CCNB1, WEE1 and β-actin were run on one gel (after stripping); while, CDK2 and β-actin were run on a separate gel. E, Cell-cycle distribution of 143B, OS-17, or hFOB cells transfected with scrambled or ALKBH5-siRNA. Propidium Iodide was used to stain for DNA content and flow cytometry analysis was done to identify cell population in different phases of cell cycle using FlowJo software. F, Bivariate cell-cycle analysis of EdU immunofluorescence versus DNA content of U2OS cells pulse-labeled with EdU. Bar graph shows quantification of EdU+ cells in scrambled and ALKBH5-siRNA–transfected U2OS cells. P values calculated using standard Student t tests. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Bar graphs represent means ±SEM for atleast three independent experiments. More
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 2327.
Published: 15 June 2022
... of glucose by growing the cells in the presence of the stable glucose isotope, UC13-Glucose and measured the C13-labelled metabolites of glycolysis and the TCA cycle. Glucose consumption was higher in endocrine therapy resistant MCF7/LCC9 (LCC9) cells compared with parental MCF7 (MCF7) and estrogen...