1-20 of 245 Search Results for

bme

Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Journal Articles
Cancer Res (2022) 82 (12_Supplement): LB516A.
Published: 15 June 2022
... generally greater cytotoxicity than gilteritinib in FLT3-ITD+ AML cell lines (MOLM-14 and MV4-11) and three FLT3-mutant AML patient samples cultured in HS5 stromal cell conditioned media that models bone marrow microenvironment (BME) induced TKI resistance. In MOLM-14s, FF-10101 more effectively suppressed...
Journal Articles
Cancer Res (2022) 82 (12_Supplement): 5847.
Published: 15 June 2022
... organoids were established from normal or cancer mammary tissues obtained from consenting patients undergoing lumpectomy or mastectomy. Isolated cells were plated in adherent basement membrane extract (BME) drops and overlaid with optimized organoid culture medium. EMT regulation in breast cancer is usually...
Journal Articles
Cancer Res canres.4390.2021.
Published: 13 June 2022
...) (Thermo Fisher Scientific), the cell pellet was dissolved in Cultrex PathClear Reduced Growth Factor BME (R&D Systems, Minneapolis, MN, USA) and seeded onto a suspension culture microplate (IWAKI, Chiba, Japan). Cells were incubated in isolation culture medium for 10 to 15 days to grow organoids...
Includes: Supplementary data
Journal Articles
Cancer Res (2022) 82 (9): 1762–1773.
Published: 03 May 2022
... for the p53 WT organoid was sequenced by the Advanced Sequencing Facility at The Francis Crick Institute. Colorectal organoids (see Supplementary Table S2) were seeded by resuspension in basement membrane extract (BME) with media (70:30 ratio). Plates were left for 30 minutes at 37°C and 5% CO2...
Includes: Supplementary data
Journal Articles
Cancer Res (2022) 82 (4_Supplement): P5-08-17.
Published: 15 February 2022
..., we identified that knockout (KO) of RALA greatly reduced the invasion of MDA-MB-231 spheroids in basement membrane extract (BME). Conversely, RALB-KO significantly increased 3D invasion of MDA-MB-231 cells. We further investigated roles for RALA and RALB in TNBC with cell viability assays, transwell...
Journal Articles
Cancer Res (2021) 81 (24): 6171–6182.
Published: 15 December 2021
...) and suspended in ENR growth medium [ADDF+++, 50x B-27 supplement (GIBCO, 17504044), 20 μg/mL EGF (Sigma-Aldrich, E4127), 500 mmol/L N-acetyl-L-cysteine (Sigma-Aldrich, A9165)] mixed 1:1 with Cultrex Reduced Growth Factor Basement Membrane Extract Type 2 (BME; Trevigen, 3533-001-02). Embedded organoids...
Includes: Supplementary data
Journal Articles
Cancer Res (2021) 81 (4): 813–815.
Published: 15 February 2021
... sites, and antitumor responses ( 8 ). Given that AML derives from clonal expansion of immature myeloid cells in bone marrow and disrupts normal hematopoiesis, it will be important to study how decitabine treatment affects the bone marrow microenvironment (BME), leukemia stem cells, and immune and other...
Journal Articles
Cancer Res (2021) 81 (5): 1308–1320.
Published: 01 March 2021
... of plasmid length at 65°C; 30 cycles) was carried out to amplify the 3′UTR fragments of interest. Following which, 1 μL of DpnI was added to the reaction mixtures and incubated at 37°C for 5 minutes. Simultaneously, competent cells were thawed, added with 2 μL of BME (β-mercaptoethanol...
Includes: Supplementary data
Images
Wnt regulates the phenotypic plasticity of CAFs.  A,  Experimental setup fo...
Published: 15 December 2020
Figure 4. Wnt regulates the phenotypic plasticity of CAFs. A, Experimental setup for RNA sequencing of fibroblasts sorted from tumors after subcutaneous transplantation of control and Sfrp1 in Rag1 mice (n = 3 each) and upon stimulation of mouse colon fibroblasts in vitro with 20% Wnt3a CM (n = 3 independent cultures each). B, GSEA of affected signatures in fibroblasts (+ Wnt vs. control; left) and sorted Pdgfrα+ CAFs (control vs. Sfrp1 tumors; right). Normalized enrichment scores (NES) are shown; asterisks indicate significant enrichment (q-value < 0.05). C, GSEA plots for myCAF and iCAF signatures in Pdgfrα+-sorted fibroblasts from control and Sfrp1 tumors. D–G, qRT-PCR analysis of iCAF and myCAF marker expression and in EMT induction in tumor organoids after in vitro Wnt modulation. D, Experimental setup. Mouse colonic fibroblasts were seeded (lower compartment) on tissue culture-treated plastic (2D) or in BME (3D) and incubated as monoculture or in coculture with APN mouse tumor organoids (top compartment) in control medium, or in presence of 5 μmol/L IWP-2 or 20% Wnt3a CM (Wnt). E and F, Relative expression of myCAF (Acta2 and Axin2; E ) and iCAF ( F ) markers (Il6, Tnfa, and Il1a) in fibroblasts. G, Relative expression of EMT markers (Zeb1, Vim, and Cdh1) in organoids. Mean normalized expression ± SEM is shown in n = 3 independent cultures per condition. Statistical significance was calculated by two-tailed unpaired t test, with P < 0.05 considered as statistically significant. All experiments were performed twice. Figure 4. Wnt regulates the phenotypic plasticity of CAFs. A, Experimental setup for RNA sequencing of fibroblasts sorted from tumors after subcutaneous transplantation of control and Sfrp1 in Rag1 mice (n = 3 each) and upon stimulation of mouse colon fibroblasts in vitro with 20% Wnt3a CM (n = 3 independent cultures each). B, GSEA of affected signatures in fibroblasts (+ Wnt vs. control; left) and sorted Pdgfrα+ CAFs (control vs. Sfrp1 tumors; right). Normalized enrichment scores (NES) are shown; asterisks indicate significant enrichment (q-value < 0.05). C, GSEA plots for myCAF and iCAF signatures in Pdgfrα+-sorted fibroblasts from control and Sfrp1 tumors. D–G, qRT-PCR analysis of iCAF and myCAF marker expression and in EMT induction in tumor organoids after in vitro Wnt modulation. D, Experimental setup. Mouse colonic fibroblasts were seeded (lower compartment) on tissue culture-treated plastic (2D) or in BME (3D) and incubated as monoculture or in coculture with APN mouse tumor organoids (top compartment) in control medium, or in presence of 5 μmol/L IWP-2 or 20% Wnt3a CM (Wnt). E and F, Relative expression of myCAF (Acta2 and Axin2; E) and iCAF (F) markers (Il6, Tnfa, and Il1a) in fibroblasts. G, Relative expression of EMT markers (Zeb1, Vim, and Cdh1) in organoids. Mean normalized expression ± SEM is shown in n = 3 independent cultures per condition. Statistical significance was calculated by two-tailed unpaired t test, with P < 0.05 considered as statistically significant. All experiments were performed twice. More
Journal Articles
Cancer Res (2020) 80 (24): 5569–5582.
Published: 15 December 2020
... setup. Mouse colonic fibroblasts were seeded (lower compartment) on tissue culture-treated plastic (2D) or in BME (3D) and incubated as monoculture or in coculture with APN mouse tumor organoids (top compartment) in control medium, or in presence of 5 μmol/L IWP-2 or 20% Wnt3a CM (Wnt). E and F...
Includes: Supplementary data
Journal Articles
Cancer Res (2020) 80 (22): 5011–5023.
Published: 13 November 2020
.... Note that 500 or 5,000 cells were seeded in 35 mm culture dishes or embedded in Matrigel (Cultrex BME) and seeded in 24-well plates. After 15 days, cell colonies were observed under a phase contrast microscope and photographed. Confluent B16-F10 cells were scraped with a 1,000 μL tip to obtain...
Includes: Supplementary data
Journal Articles
Cancer Res (2020) 80 (21): 4741–4753.
Published: 03 November 2020
... Scientific; 1× B27, Thermo Fisher Scientific; 1× N2, Thermo Fisher Scientific; 0.1 mmol/L BME, Sigma; 2 μg/mL heparin, Stemcell Technologies; 1% penicillin–streptomycin, Gemini; 20 ng/mL EGF, Corning; and 40 ng/mL FGF, Corning) and cultured in Ultra-Low Attachment Plates (Corning). Neurospheres were...
Includes: Supplementary data
Journal Articles
Series: Author Choice
Cancer Res (2020) 80 (20): 4355–4370.
Published: 15 October 2020
... treated for 24 hours with 1 μmol/L of Dexamethasone (DEX), 8 μmol/L SH4–54, no treatment, or combination of DEX and SH4–54. The cells were lysed using 350 μL of Qiagen RLT buffer and 1% BME. RNA was extracted using Norgen Animal Tissue RNA Purification Kit according to the manufacturer's instructions. RNA...
Includes: Supplementary data
Journal Articles
Cancer Res (2020) 80 (11_Supplement): A21.
Published: 02 June 2020
... with collagenase and dispase to release epithelium. Glands or epithelia cells were collected, resuspended in BME (basement membrane extract), and plated in 2 wells of 24-well chambers. On day 4-6, PDOs were dissociated and passaged using manual pipetting. For passage 2 on day 9-11, PDOs were isolated into smaller...
Journal Articles
Series: Author Choice
Cancer Res (2020) 80 (17): 3719–3731.
Published: 02 September 2020
... in Luria broth (LB) to OD600 0.9 and induced with 0.5 mol/L isopropyl β-D-1-thiogalactopyranoside (IPTG) for 16 hours at 16°C. Cells were pelleted and resuspended in lysis buffer [20 mmol/L sodium phosphate (pH 8.0), 500 mmol/L NaCl, 10 mmol/L imidazole, 1 mmol/L 2-mercaptoethanol (BME), 5% (v/v) glycerol...
Includes: Supplementary data
Journal Articles
Cancer Res (2020) 80 (4): 663–674.
Published: 14 February 2020
... Research Development and Innovation Office 10.13039/501100011019 K116295 Tovari J. Research and Technology Innovation Fund of Hungary FIEK_16-1-2016-0005 Szakács G. National Excellence Program ÚNKP-18-4-BME-391 Szabó J.E. 2019 Thematic Excellence Program TUDFO...
Includes: Supplementary data
Images
<em>Cn<sup>−/−</sup></em> fibroblasts promote endothelial cell tube...
Published: 01 August 2019
Figure 5. Cn−/− fibroblasts promote endothelial cell tube formation. A, Representative images from tube formation assay with WT versus Cn−/− fibroblast + GFP+ EC (green) cocultures embedded in BME at 24 hours following plating. Images are from one representative experiment (N = 2 images from one replicate for quantification) of six experimental replicates. Quantification was performed on phase images using the Angiogenesis Analyzer tool in ImageJ. B, Conditioned media from WT or Cn−/− fibroblasts cultured on BME for 24 hours was analyzed with an angiogenesis antibody array. Cytokines with statistically significant differences between WT and Cn−/− fibroblasts (P < 0.05) are quantified on right. **, P < 0.01; ***, P < 0.001. N = 2 per condition; array contained technical duplicates for each cytokine. Error bar, SD. Figure 5. Cn−/− fibroblasts promote endothelial cell tube formation. A, Representative images from tube formation assay with WT versus Cn−/− fibroblast + GFP+ EC (green) cocultures embedded in BME at 24 hours following plating. Images are from one representative experiment (N = 2 images from one replicate for quantification) of six experimental replicates. Quantification was performed on phase images using the Angiogenesis Analyzer tool in ImageJ. B, Conditioned media from WT or Cn−/− fibroblasts cultured on BME for 24 hours was analyzed with an angiogenesis antibody array. Cytokines with statistically significant differences between WT and Cn−/− fibroblasts (P < 0.05) are quantified on right. **, P < 0.01; ***, P < 0.001. N = 2 per condition; array contained technical duplicates for each cytokine. Error bar, SD. More
Journal Articles
Cancer Res (2019) 79 (13_Supplement): 43.
Published: 01 July 2019
... (Hsp90) were used for drug sensitivity screens through CCK-8 assay. Results For establishing a living TNBCs tissues from patients, tissue samples were operated to isolate cells through mechanical and enzymatic digestion. Digested cells were resuspended with BME drops and seeded into organoid culture...
Journal Articles
Cancer Res (2019) 79 (15): 3928–3939.
Published: 01 August 2019
...Figure 5. Cn−/− fibroblasts promote endothelial cell tube formation. A, Representative images from tube formation assay with WT versus Cn−/− fibroblast + GFP+ EC (green) cocultures embedded in BME at 24 hours following plating. Images are from...
Includes: Supplementary data
Journal Articles
Cancer Res (2019) 79 (4_Supplement): P2-01-08.
Published: 15 February 2019
... of bone metastasis of breast cancer. Materials and Methods: In order to identify potential genes involved in bone metastasis, we established a novel in vitro model named Bone Matrix Extract (BME) which was extracted from human femur and used to mimic the bone environment. Full profile of gene...