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apoptosis

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Journal Articles
Cancer Res (2022) 82 (9): 1844.
Published: 03 May 2022
... A , Fenollar-Ferrer C , Napoli M , Anselmi C , Girardini JE , . Peptide aptamers targeting mutant p53 induce apoptosis in tumor cells . Cancer Res 2008 ; 68 : 6550 – 8 . ...
Journal Articles
Journal Articles
Cancer Res (2022) 82 (7): 1208–1221.
Published: 01 April 2022
... apoptosis in cancer cells while not affecting normal cells. These results indicate that G9a and EZH2 promotes the evasion of ER stress–mediated apoptosis by repressing IL24 transcription, therefore suggesting that their inhibition may represent a potential therapeutic strategy for solid cancers...
Includes: Supplementary data
Journal Articles
Cancer Res (2022) 82 (4_Supplement): P5-10-06.
Published: 15 February 2022
...-Arginase induced apoptosis that was associated with cell morphological changes, histone release from fragmented DNA, caspase-3 activation, and cell cycle arrest at S and/or G2/M phases in a dose-. dependent manner after 48h. Moreover, Peg-Arginase down-regulated miR-23a, STAT3, β-catenin, cyclin D1...
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METTL3 recovers PD-L1 expression depressed by JNK inhibition in bladder can...
Published: 03 May 2022
Figure 6. METTL3 recovers PD-L1 expression depressed by JNK inhibition in bladder cancer cells. A – D, qPCR analysis ( A ), Western blot ( B ), m6A-qPCR assay ( C ), and luciferase assay ( D ) indicate PD-L1 expression and m6A level in 5637 cells where JNK inhibition was rescued by exogenous METTL3. E, Cytotoxicity of activated CD8+ T cells on cocultured 5637 cells was measured by LDH assay. F, Analysis of 5637 cell apoptosis while cocultured with activated CD8+ T cells by flow cytometry. G – L, Cytotoxicity of activated CD8+ T cells on cocultured 5637 cells ( G, I, and K ) and cell apoptosis of 5637 cells ( H , J, and L ) transfected with indicated plasmid or siRNAs was measured by LDH assay and flow cytometry, respectively. Data are presented as mean ± SD of three independent experiments, with individual data points shown. P values for the differences between indicated groups were assessed by two-tailed Student t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Figure 6. METTL3 recovers PD-L1 expression depressed by JNK inhibition in bladder cancer cells. qPCR analysis (A), Western blot (B), m6A-qPCR assay (C), and luciferase assay (D) indicate PD-L1 expression and m6A level in 5637 cells where JNK inhibition was rescued by exogenous METTL3. E, Cytotoxicity of activated CD8+ T cells on cocultured 5637 cells was measured by LDH assay. F, Analysis of 5637 cell apoptosis while cocultured with activated CD8+ T cells by flow cytometry. Cytotoxicity of activated CD8+ T cells on cocultured 5637 cells (G, I, and K) and cell apoptosis of 5637 cells (H, J, and L) transfected with indicated plasmid or siRNAs was measured by LDH assay and flow cytometry, respectively. Data are presented as mean ± SD of three independent experiments with individual data points shown. P values for the differences between indicated groups were assessed by two-tailed Student t test. *, **, and *** indicate P < 0.05, 0.01, and 0.001 respectively. NS, not significant. More
Journal Articles
Cancer Res (2022) 82 (10_Supplement): A027.
Published: 15 May 2022
.... Fluorescence labeled gefitinib-resistant PC-9 cells and naïve parental PC-9 cells are co-cultured in a closed chamber with limited availability to oxygen and nutrient supply at one end. A green hypoxia probe is used to verify the establishment of a hypoxia gradient and a red Annexin V probe monitors apoptosis...
Journal Articles
Cancer Res (2022) 82 (10_Supplement): B009.
Published: 15 May 2022
... damage to induce apoptosis. As a result, adaptive therapy algorithms that modulate treatment but never completely withdraw it are predicted to perform better in this setting than strategies based on treatment interruptions. Subsequent experiments confirm this prediction in vivo. To conclude, we...
Journal Articles
Cancer Res (2022) 82 (10_Supplement): PR015.
Published: 15 May 2022
... in vitro in a resource-poor environment. The competitive effect was removed on administration of chemotherapy but restored upon re-growth of the sensitive cell population. Flow cytometry for annexin V revealed that resistant cells undergo more apoptosis when cultured with sensitive cells, which increased...
Journal Articles
Cancer Res canres.3910.2021.
Published: 11 May 2022
..., and apoptosis. Here, we sought to identify cancer-associated micropeptides and to uncover their mechanistic functions. A micropeptide named short trans-membrane protein 1 (STMP1) that localizes at the inner mitochondrial membrane was identified to be upregulated in various cancer types and associated...
Includes: Supplementary data
Journal Articles
Cancer Res (2022) 82 (10): 1847–1848.
Published: 16 May 2022
... that high expression and activity of these enzymes is specific to cycling (i.e., activated) B cells. Knockout or inhibition of PHGDH in human cells and mouse models results in impaired antibody production and immune response, as well as induction of apoptosis in lymphomas derived from this compartment...
Journal Articles
Cancer Res (2022) 82 (10): 1909–1925.
Published: 16 May 2022
... regardless of gemcitabine treatment. EC apoptosis, was not detectable by double immunostaining for cleaved caspase 3 and PECAM in primary 8661 tumors, from treated or untreated EC-FAKWT or EC-FAKKO mice (Supplementary Fig. S1F). Figure 1. EC-FAK deletion in mice with established...
Includes: Supplementary data
Journal Articles
Cancer Res (2022) 82 (10): 1890–1908.
Published: 16 May 2022
... hours postinjection, Texas red-dextran 70 kDa was injected retro-orbitally 30 minutes prior to sacrifice. Four to 6 sections per mouse (from different regions) were evaluated for apoptosis rates in GFP+ cells. MH6620c1 subcutaneous tumors were established by inoculation of 3...
Includes: Multimedia, Supplementary data
Journal Articles
Cancer Res (2022) 82 (10): 1858–1869.
Published: 16 May 2022
... integrity. As a tumor grows, areas of tumor that outgrow the nutrient and oxygen supply generate a necrotic milieu. Greater efficacy in vivo with the APOMAB conjugate was seen with chemotherapy prior to ADC therapy (resulting in apoptosis/necrosis with greater availability of La/SSB) and only...
Images
JNK regulates m<sup>6</sup>A and expression level of PD-L1.  A – D,  qPCR a...
Published: 03 May 2022
Figure 5. JNK regulates m6A and expression level of PD-L1. A – D, qPCR and Western blot showing PD-L1 expression in bladder cancer cells treated with SP600125 or siRNAs targeting different JNKs. E, Knockdown of JNK1 by two different siRNAs in 5637 cells verified by qPCR. F, Relative luciferase activity of pGL3-PDL1–3UTR after cotransfection with JNK1 siRNAs into 5637 cells. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. G and H, Reduction of m6A modification in specific regions of PD-L1 transcripts upon knockdown of indicated JNK isoforms ( G ) and JNK signaling inhibition ( H ) were examined by gene-specific m6A-qPCR assay. I, Analysis of 5637 cell apoptosis while transfected with indicated siRNAs and cocultured with/without activated CD8+ T cells by flow cytometry. J, Cytotoxicity of activated CD8+ T cells on cocultured 5637 cells transfected with indicated siRNAs. Data are presented as mean ± SD of three independent experiments, with individual data points shown. P values were assessed by two-tailed Student t test in comparison with the control group, which was treated with the vehicle (DMSO; A and H ) or the Scramble group ( C, E–G, I, and J ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Figure 5. JNK regulates m6A and expression level of PD-L1. A to D, qPCR and Western blot showing PD-L1 expression in bladder cancer cells treated with SP600125 or siRNAs targeting different JNKs. E, Knockdown of JNK1 by two different siRNAs in 5637 cells verified by qPCR. F, Relative luciferase activity of pGL3-PDL1–3UTR after cotransfection with JNK1 siRNAs into 5637 cells. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. G and H, Reduction of m6A modification in specific regions of PD-L1 transcripts upon knockdown of indicated JNK isoforms (G) and JNK signaling inhibition (H) were examined by gene-specific m6A-qPCR assay. I, Analysis of 5637 cell apoptosis while transfected with indicated siRNAs and cocultured with/without activated CD8+ T cells by flow cytometry. J, Cytotoxicity of activated CD8+ T cells on cocultured 5637 cells transfected with indicated siRNAs. Data are presented as mean ± SD of three independent experiments with individual data points shown. P values were assessed by two-tailed Student t test in comparison with the control group which was treated with the vehicle (DMSO; A, H) or the Scramble group (C, E–G, I–J). *, **, and *** indicate P < 0.05, 0.01, and 0.001 respectively. More
Journal Articles
Cancer Res (2022) 82 (10): 1991–2002.
Published: 16 May 2022
... compromised immunosurveillance, but on the other, chemotherapy can induce an immunosuppressive TME by inducing a series of complex protumor signaling events, which instead drives tumor development ( 3–5 ). Chemotherapy-induced tumor cell death frequently occurs via apoptosis, and the apoptotic tumor cells...
Includes: Supplementary data
Journal Articles
Cancer Res canres.3844.2021.
Published: 18 May 2022
... and immunoblotting showed high CD133 expression in cisplatin resistance of KATO-III cells (Cis-KATO-III) compared to parental KATO-III cells, indicating that CD133 regulates cisplatin resistance in KATO-III cells. Furthermore, proliferation and apoptosis assays on flow-sorted CD133+ve Cis-KATO-III cells that were...
Images
PD-L1 is regulated by METTL3-mediated m<sup>6</sup>A modification.  A,  mRN...
Published: 03 May 2022
Figure 1. PD-L1 is regulated by METTL3-mediated m6A modification. A, mRNA m6A level in four bladder cancer cell lines (5637, J82, UM-UC-3, and T24) and one normal urothelial cell line, SV-HUC-1, was determined by RNA m6A quantification kit. B, The abundance of m6A on PD-L1 mRNA transcripts in 5637 cells as detected by m6A-seq was plotted using Integrative Genomics Viewer. The y-axis shows sequence read number, blue boxes represent exons, and blue lines represent introns. C and D, mRNA level ( C ) and protein level ( D ) of PD-L1 in 5637 and J82 cells transfected with indicated siRNAs were examined by qPCR and Western blot. E, Knockdown of METTL3 by two different siRNAs in 5637 and J82 cells was verified by qPCR. F, Relative luciferase activity of pGL3-PDL1–3UTR after cotransfection with indicated METTL3 siRNAs in 5637 or J82 cells. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. G, Cytotoxicity of activated CD8+ T cells on cocultured 5637 or J82 cells transfected with METTL3 siRNAs. H, Analysis of 5637 or J82 cell apoptosis after coculture with activated CD8+ T cells by flow cytometry. Data are presented as mean ± SD of no less than three independent experiments, with individual data points shown. P values were assessed by two-tailed Student t test ( A, C, and E–G ) in comparison with SV-HUC-1 ( A ) or the Scramble group ( C and E–G ). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Figure 1. PD-L1 is regulated by METTL3 mediated m6A modification. A, mRNA m6A level in four bladder cancer cell lines (5637, J82, UM-UC-3, and T24) and one normal urothelial cell line, SV-HUC-1, were determined by RNA m6A quantification kit. B, The abundance of m6A on PD-L1 mRNA transcripts in 5637 cells as detected by m6A-seq is plotted using Integrative Genomics Viewer (IGV). The y-axis shows sequence read number, blue boxes represent exons, and blue lines represent introns. mRNA level (C) and protein level (D) of PD-L1 in 5637 and J82 cells transfected with indicated siRNAs were examined by qPCR and Western blot. E, Knockdown of METTL3 by two different siRNAs in 5637 and J82 cells were verified by qPCR. F, Relative luciferase activity of pGL3-PDL1–3UTR after cotransfection with indicated METTL3 siRNAs in 5637 or J82 cells. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. G, Cytotoxicity of activated CD8+ T cells on cocultured 5637 or J82 cells transfected with METTL3 siRNAs. H, Analysis of 5637 or J82 cell apoptosis after coculture with activated CD8+ T cells by flow cytometry. Data are presented as mean ± SD of no less than three independent experiments with individual data points shown. P values were assessed by two-tailed Student t test (A, C, E – G) in comparison with SV-HUC-1 (A) or the Scramble group (C, E, –G). *, **, and *** indicate P < 0.05, 0.01 and 0.001 respectively. More
Journal Articles
Cancer Res canres.3896.2021.
Published: 17 May 2022
... extravasation of 300 MDSCs across tumor vasculature (Fig. 4C-E). The ratio of labeled Cldn12 to 301 Cldn12 MDSCs increased from 1 to 1 in the mixture to 1.77 to 1 in the recovered 302 sample after 48 h (Fig. 4F). In addition, the proliferation (BrdU+ in CD11b+Gr1+ cells) 303 or apoptosis (Annexin V+ in CD11b...
Includes: Supplementary data
Journal Articles
Cancer Res OF1–OF9.
Published: 09 May 2022
... have been shown to be ineffective long term because of acquired resistance, as these agents cause a selective pressure that increases the frequency of CSCs (68). The drug resistance mechanisms include the development of resistance to apoptosis, increased DNA damage repair ef ciency, elimination...
Journal Articles
Cancer Res (2022) 82 (10): 1926–1936.
Published: 16 May 2022
... to inhibition by MDM2 and MDM4 proteins ( 2 ). Under DNA damage and other stress signals, p53 is posttranslationally modified and stabilized to activate a transcriptional repertoire that induces cell-cycle arrest, senescence, apoptosis, and metabolic changes to inhibit cell transformation ( 3, 4 ). Given...
Includes: Supplementary data