Shining a light on cholangiocarcinoma. NanoString imaging highlights spatial interactions between tumor cells (green), T cells (red), and stromal cells (yellow) within the cholangiocarcinoma tumor microenvironment. The recruitment of immune cells, activation of stromal cells, and proliferation of epithelial cells is a feature of cholangiocarcinoma. Dysregulation of WNT and NOTCH signaling pathways is critical to proliferation and invasion and occurs through activation of the PRH transcription factor, which is thereby able to increase epithelial-mesenchymal plasticity and transit through the cell cycle. Image collected by Dr. Jingjing Gong (NanoString), Dr. Isioma Egbuniwe (histopathologist, University of Nottingham, United Kingdom), Professor K. Gaston (University of Nottingham), and Dr. P.S. Jayaraman (University of Birmingham, United Kingdom). For details, see article by Kitchen and colleagues on page 757.