Plasma membrane citrate transporter (pmCiC), a variant of the mitochondrial SLC25A1 carrier, was found to be expressed in human cancer cells of different origin and responsible for extracellular citrate uptake. Extracellular citrate supports cancer cell metabolism by reducing the need of synthesizing citrate in mitochondria and by promoting fatty acid synthesis. In vivo application of gluconate, a specific and irreversible pmCiC inhibitor, was found to reduce subcutaneous human pancreatic tumor growth in mice and to change metabolic characteristics of the tissue. pmCiC is shown as a molecular envelope (light blue) created from a homology model. The membrane is shown as a stick model (beige), with gluconate (green) and citrate (yellow) shown as space-filling models; heteroatoms are color-coded: oxygen, red; nitrogen, blue; phosphorus, orange. The figure was prepared using UCSF Chimera. The figure was prepared by Gregor Madej (University of Regensburg, Regensburg, Bavaria). For details, see article by Mycielska and colleagues on page 2513.