Timing of GLA in relation to vaccination impacts the pattern of OT1 cell accumulation. Representative bioluminescent images show site-specific accumulation of OT1 cells in different groups of mice 4 days post hind footpad vaccination. Vaccine-primed T cells accumulated in the draining lymph nodes in mice that received GVAX only or when GLA 24 was given 24 hrs post GVAX. However, when GLA is coadministered with GVAX, a systemic pattern of T-cell accumulation was observed. For details, see article by Kadayakkara and colleagues on page 51.