Attempts were made to develop a more rapid method of testing for the presence of the mouse mammary tumor milk agent.

Repeated doses of filtrates of freshly excised spontaneous C3H mammary tumors failed to induce mammary tumors in 10 to 15 months old BDF1 and BAF1 breeding females. The mice which previously had been submitted to splenectomy and vital staining with trypan blue or congo red were kept under observation for 8 to 18 weeks, until the experiments were accidentally interrupted.

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This investigation was aided by a Finney-Howell Research Foundation Fellowship.

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