Methods are outlined for fractionating small volumes of plasma into fibrinogen, globulin, and albumin-rich fractions by the low temperature-ethanol procedures.

Electrophoretic, nitrogen, lipide carbon, cholesterol and enzyme analyses of the plasma and three plasma fractions of 17 healthy young men and of 36 patients with carcinoma of the prostate, stomach, pancreas, bronchus, breast, colon, ovary, and larynx; lymphoblastoma, myelogenous leukemia, and hepatoma are presented. The electrophoretic and nitrogen analyses show: (a) a consistent decrease in albumin, (b) an increase in fibrinogen. and (c) few significant changes in the globulin components. The changes in the lipides of plasma and its fractions are due to cachexia or hepatic involvement. The distributions of the alkaline and acid phosphatases and amylase of plasma fractions are discussed.

The results presented for the plasma protein fractions do not yield information which is of value in the diagnosis of cancer.

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This investigation was supported by research grants from the National Cancer Institute, U.S. Public Health Service, and from the Office of Naval Research.

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