Long noncoding RNAs (lncRNA) are dysregulated in various human cancers and control tumor development and progression. However, the upstream mechanisms underlying their dysregulation remain unclear. Here, we demonstrate that the expression of hepatocyte nuclear factor 1 homeobox A antisense RNA 1 (HNF1A-AS1) is significantly upregulated in gastric cancer tissues. Overexpression of HNF1A-AS1 enhanced cell proliferation and promoted cell-cycle progression, whereas knockdown of HNF1A-AS1 elicited the opposite effects. Early growth response protein 1 (EGR1) directly bound the HNF1A-AS1 promoter region and activated its transcription. Overexpression of EGR1 enhanced cell proliferation and promoted cell-cycle promotion, similar to the function of HNF1A-AS1. HNF1A-AS1 functioned as competing endogenous RNA (ceRNA) by binding to miR-661, upregulating the expression of cell division cycle 34 (CDC34), which is a direct target of miR-661. EGR1 and HNF1A-AS1 enhanced the expression of cyclin-dependent kinase 2 (CDK2), CDK4, and cyclin E1 but inhibited the expression of p21 by promoting CDC34-mediated ubiquitination and degradation of p21. Taken together, these findings suggest that EGR1-activated HNF1A-AS1 regulates various pro- and antigrowth factors to promote the development of gastric cancer, implicating it as a possible target for therapeutic intervention in this disease.

Significance: This study provides novel insights into mechanisms by which the noncoding RNA HNF1A-AS1 contributes to gastric cancer progression through modulation of the cell cycle. Cancer Res; 78(20); 5877–90. ©2018 AACR.

Gastric cancer remains the fourth most frequently diagnosed cancer and is the third leading cause of cancer-related deaths worldwide (1, 2). Cell proliferation is one characteristic of cancer development and progression, and high cellular proliferation is associated with a poor prognosis in patients with malignant tumors (3). With progress in medical care, several screening techniques, including barium meal photofluorography, gastric endoscopy, and serum pepsinogen, have been recommended as screening methods for the detection of early gastric cancer. Nevertheless, some patients are still diagnosed at advanced stages (4), which often have poor prognosis mainly due to the progression of the gastric cancer and tumor growth (5). Therefore, novel insights into the mechanisms underlying gastric cancer progression are warranted, which in turn may identify potential therapeutic targets that to improve patient survival.

Recent transcriptomic studies have shown that the majority of the human genome is transcribed into noncoding RNA molecules (6, 7). Long noncoding RNAs (lncRNA) are noncoding transcripts that are larger than 200 nucleotides and lack protein-coding potential. Compared with protein-coding transcripts, lncRNA genes have fewer exons, are of lower abundance, and are highly cell- and tissue specific (8). LncRNAs regulate gene expression at the transcriptional, posttranscriptional, and epigenetic levels by interacting with various biomolecules, including DNA, RNA, and proteins (9). There is a growing body of evidence that these noncoding transcripts participate in a wide range of cellular processes, including cell proliferation (10), apoptosis (11), differentiation (12), and the immune response (13), all of which are involved in tumorigenesis. Thus, studying the expression of lncRNAs could be of profound value with regard to understanding the mechanisms underlying gastric cancer progression.

Among all known cancer-related lncRNAs, hepatocyte nuclear factor 1 homeobox A antisense RNA 1 antisense RNA 1 (HNF1A-AS1) expression is upregulated in various malignancies (14–17), and its elevated expression is associated with an advanced stage and/or poor prognosis, indicating an oncogenic role for HNF1A-AS1 in tumor progression. Wu and colleagues reported that HNF1A-AS1 could regulate cyclin D1, E-cadherin, N-cadherin, and β-catenin expression, inducing the EMT process, thereby promoting lung adenocarcinoma proliferation and metastasis (18). Additionally, HNF1A-AS1 functions as an oncogene in colon cancer cells by acting as a ceRNA for miR-34a, which then leads to desuppression of SIRT1, thereby facilitating colon cancer progression (19). However, no studies on the upstream regulatory mechanisms of HNF1A-AS1 and promoter analysis of HNF1A-AS1 have been reported to date.

In this study, we demonstrate that HNF1A-AS1 is upregulated in gastric cancer tissues and promotes gastric cancer malignant phenotypes. Early growth response protein 1 (EGR1) transcriptionally upregulates HNF1A-AS1, which acts as a ceRNA by competitively binding to microRNA (miR)-661 to enhance the expression of cell division cycle 34 (CDC34). Additionally, we show that HNF1A-AS1 promotes gastric cancer cell proliferation and cell-cycle progression, in which cyclin-dependent kinase 2 (CDK2), CDK4, and cyclin E1 are involved and induces the dysregulation of P21 protein via CDC34-mediated ubiquitination.

Tissue specimens and cell lines

A total of 99 fresh gastric cancer tissues and 8 nontumorous gastric tissues were obtained from the Qilu Hospital of Shandong University (Shandong Province, China). Our study was approved by the Institute's Research Ethics Committee of Shandong University and conducted in accordance with the ethical guidelines of the World Medical Association Declaration of Helsinki. Informed consent was written by all patients prior to this study. All the cells were purchased from the ATCC. Cell authentication was verified by short tandem repeat profiling. Human MKN-45 cells and BGC-823 cells were cultured in RPMI-1640 medium (Gibco) containing 10% fetal bovine serum (FBS; Gibco) at 37°C, 5% CO2. HEK293 cells were cultured in Dulbecco's Modified Eagle Medium (Gibco) supplemented with 10% FBS. Mycoplasma PCR testing of these cells was performed every month. Cells were grown for no more than 20 passages in total for any experiment.

siRNA and cell transfection

Two siRNA targeting HNF1A-AS1 (si-HNF1A-AS1-1 and si-HNF1A-AS1-2) and scrambled negative control (si-NC) were designed and synthesized by RiboBio. The sequence of si-HNF1A-AS1-1 and si-HNF1A-AS1-2 was as follows: CCCTCCATCTAACATTCAA; CCCTCATCCTGGCATTCAA. MKN-45 and BGC-823 cells were seeded in 6- and 12-well plates and transfected 48 hours later using X-tremeGENE transfection reagent (Roche Applied Science) according to the manufacturer's instructions.

RNA extraction and RT-qPCR

Total RNAs were extracted from the tissues and cultured cells using TRIzol (Invitrogen) in accordance with the manufacturer's instructions. Approximately 1 μg of total RNA was reverse transcribed to cDNA using a reverse transcriptase cDNA synthesis kit (Toyobo), and qPCR was performed using a SYBR Green PCR kit (Roche). Comparative quantification was assessed using the 2−ΔCt method using GAPDH as the endogenous control.

Gastric cancer cell viability and proliferation assay

The MTS, colony formation, and EdU cell proliferation assays were performed to evaluate the viability and proliferative capacity of gastric cancer cells as previously described (20).

Migration and invasion assays

Transwell chambers either uncoated or coated with Matrigel were used to observe gastric cancer cell migration and invasion as described elsewhere (21).

Flow cytometry analysis of the cell cycle and apoptosis

MKN-45 and BGC-823 cells transfected with HNF1A-AS1-PCDNA3.1 or Si-HNF1A-AS1 were cultured in 6-well plates for 48 hours. To evaluate the cell cycle, the cells were stained with propidium iodide (PI; Beyotime). To evaluate apoptosis, the cells were stained with Annexin V fluorescein isothiocyanate (FITC) and PI using an FITC Annexin V Apoptosis Detection Kit. Harvested cells were analyzed by flow cytometry (FACScan; BD Biosciences) according to the manufacturer's recommendations.

Bioinformatics analysis

The HNF1A-AS1 sequence was downloaded from UCSC Genome Browser (http://genome.ucsc.edu/), from which the 2,000-bp transcription start site (TSS) upstream sequence was extracted. To identify putative transcription factors, the promoter sequence of HNF1A-AS1 was submitted to the JASPAR program (http://jaspar.genereg.net/).

Vector construction

The putative HNF1A-AS1 promoter regions (−2,000/0, −1,014/0, −503/0, −285/0, and −187/0) were PCR amplified from the genomic DNA of MKN-45 cells, which were then inserted into the HindIII–NheI sites upstream of the firefly luciferase in the pGL3-Basic vector (Promega). All of the constructs were named based on the location of the promoter fragments relative to the TSS. The full-length cDNA sequence of KLF5, ELK1, and EGR1 was PCR amplified from the cDNA of MKN-45 cells and cloned into the pcDNA3.1 vector. The HNF1A-AS1 expression vector PCDNA3.1-HNF1A-AS1 was generated in our previous study. Ubiquitin plasmid was generously provided by Dr. Hu (Shandong University). P21, CDC34, and SKP2 vectors were purchased from Vigene Biosciences. The 3′-UTR of CDC34 was subcloned into the pmirGLO vector and named pmiRGLO-CDC34. All vectors were confirmed by direct sequencing using primers shown in Supplementary Table S1.

HNF1A-AS1 short hairpin RNA stable knockdown

Short hairpin RNAs (shRNA) targeting HNF1A-AS1 were selected and expressed using the pGPU6/GFP/Neo vector (GenePharma). The shRNA sequence was as follows: CCCTCCATCTAACATTCAA. The MKN-45 and BGC-823 cells were seeded into 6-well plates and transfected using Lipofectamine 2000 (Invitrogen). To screen stable HNF1A-AS1 knockdown cells, G418 (500 μg/mL) was added to the medium 72 hours after transfection. The knockdown efficiency of HNF1A-AS1 is shown in Supplementary Fig. S1A and S1B.

Dual-luciferase reporter assays

The HEK293 cells were seeded overnight in 24-well plates and then cotransfected per well 0.5 μg of the overexpression vector, 0.5 μg of the HNF1A-AS1 promoter-luciferase reporter plasmid, and 0.01 μg of pRL-TK plasmid with Lipofectamine 2000 (Invitrogen). PRL-TK was used as the internal control. Approximately 48 hours after transfection, the cells were subjected to luciferase activity analysis using a Dual-Luciferase Reporter Assay System (Promega) following the manufacturer's instructions.

Chromatin immunoprecipitation assay

Chromatin immunoprecipitation (ChIP) assays were performed using the EZ-Magna ChIP Chromatin Immunoprecipitation Kit (Millipore) according to the manufacturer's instructions. Briefly, the MKN-45 and BGC-823 cells in each immunoprecipitation reaction were crosslinked with 1% formaldehyde for 10 minutes at room temperature. After incubation with anti-EGR1 (Cell Signaling Technology [CST]) or IgG antibody and protein A/G magnetic beads overnight, immunoprecipitation was performed. Coprecipitated DNA was quantified using PCR and qPCR. The HNF1A-AS1 primers used are shown in Supplementary Table S1.

Actinomycin D and cycloheximide assays

The HNF1A-AS1 and CDC34 plasmids were transiently transfected into gastric cancer cells using Lipofectamine 2000 (Invitrogen). After 24 hours, actinomycin D (ActD, 5 μg/mL) or cycloheximide (5 μg/mL) was added to the culture medium, followed by incubation for 15 minutes, 30 minutes, 1 hour, 2 hours, or 3 hours. P21 mRNA stability in the ActD treatment group was analyzed by qPCR. The half-life of p21 protein in the cycloheximide treatment group was observed by Western blotting.

Western blotting

The antibodies used for Western blotting were rabbit anti-CDK2 (CST), rabbit anti-CDK4 (CST), rabbit anti-cyclin E1 (CST), rabbit anti-p21 (Abways), rabbit anti-SKP2 (CST), and rabbit anti-P27 (CST).

In vitro ubiquitination analysis

HNF1A-AS1, ubiquitin, and p21 plasmids were transfected into cells using Lipofectamine 2000. Approximately 48 hours after transfection, the lysates were immunoprecipitated with p21 antibodies (Sigma-Aldrich) overnight at 4°C with gentle rotation. The immunoprecipitated proteins were analyzed by Western blotting.

RNA binding protein immunoprecipitation assays

RNA binding protein immunoprecipitation (RIP) assays were performed using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Millipore) according to the manufacturer's instructions. Briefly, the cells were lysed in lysis buffer containing a protease inhibitor cocktail and RNase inhibitor, and the lysates were immunoprecipitated with anti-Ago2 and IgG antibodies. Finally, the retrieved RNAs were quantified by qPCR. The HNF1A-AS1 and CDC34-3′ UTR primers used for amplification are listed in Supplementary Table S1.

Biotin-labeled pulldown assays

The BGC-823 cells were transfected with biotinylated miR-661 and a scrambled control miRNA (GenePharma) and collected 48 hours after transfection. The cell lysates were incubated with M-280 streptavidin magnetic beads (Invitrogen) and 10 μL of yeast tRNA on a rotator at 4°C for 2 hours. The bound RNAs were purified by adding 750 μL of TRIzol (Invitrogen) per sample and 250 μL of water to the input and the pulldown beads for further RT-qPCR analysis. The 3′ biotin-labeled miR-661 sequence was as follows: 5′-UGCCUGGGUCUCUGGCCUGCGCGU-3′ BiO. The scrambled control miRNA sequence was as follows: 5′-UUCUCCGAACGUGUCACGUTT-3′BiO.

IHC staining

Fresh gastric cancer tissue sections were cut into 4-μm sections and then analyzed by IHC using antibodies against EGR1 (1:50; CST). Brown signals in the nuclei and cytoplasm indicated positive EGR1 immunostaining. EGR1 staining intensity was defined using a three-scale grading system: weak (score: 1), moderate (score: 2), or strong (score: 3). A staining score was calculated as follows: Score (maximum of 300) = sum of 1 × percentage of weak, 2 × percentage of moderate, and 3 × percentage of strong staining. Then, the relationship between EGR1 levels and lncRNA HNF1A-AS1 was assessed for each sample.

Tumor xenograft model

Four-week-old male Nu/nu athymic nude mice (Weitonglihua Biotechnology) were raised under specific pathogen-free conditions. LV-HNF1A-AS1 and LV-NC-MKN-45–transfected cells were, respectively, injected subcutaneously into the flank region of the mice (7 × 105 cells/150 μL per flank, n = 8). The tumor volumes were measured every 7 days after inoculation. Five weeks after injection, the mice were sacrificed, and the tumor nodules were harvested. The tumors, lungs, liver, and kidneys were isolated from the mice for further analysis. The protocol was approved by the Committee on the Ethics of Animal Experiments of the Shandong University.

Statistical analysis

All data were statistically analyzed using GraphPad Prism 5 software. Analysis of the differences between the two groups was determined by the Student t test. The correlation between EGR1 levels and HNF1A-AS1 was assessed using Spearman correlation coefficient. A P value of less than 0.05 was considered statistically significant.

HNF1A-AS1 promotes gastric cancer cell growth and invasion

In our study, we found that HNF1A-AS1 expression was significantly upregulated in gastric cancer tumor tissues compared with nontumorous gastric tissues (Fig. 1A). To further explore the biological functions of HNF1A-AS1 in gastric cancer cells, the gastric cancer cells were transfected with the pcDNA3.1-HNF1A-AS1 plasmid or small interfering RNA (siRNA) targeting HNF1A-AS1 (si-HNF1A-AS1-1 and si-HNF1A-AS1-2). The overexpression and knockdown efficiency of HNF1A-AS1 were detected 48 hours after transfection (Supplementary Figs. S1C–H). EdU assays showed that HNF1A-AS1 overexpression enhanced cell proliferation (Fig. 1B), whereas HNF1A-AS1 knockdown significantly suppressed cell growth (Fig. 1C; Supplementary Fig. S1I). Coinciding with our EdU results, the MTS assay also showed that HNF1A-AS1 enhances cell proliferation (Fig. 1D–G; Supplementary Fig. S1J–K). Additionally, colony formation assays revealed that the number and size of all colonies increased in the HNF1A-AS1–overexpression groups (Fig. 1H), indicating that HNF1A-AS1 also promotes the anchorage-independent growth of gastric cancer cells. HNF1A-AS1 downregulation effectively repressed anchorage-independent growth (Fig. 1I; Supplementary Fig. S1L). In addition, the overexpression of HNF1A-AS1 significantly promoted gastric cancer cell migration and invasion (Fig. 1J; Supplementary Fig. S1M), whereas HNF1A-AS1 knockdown markedly suppressed migration and invasion (Fig. 1K; Supplementary Fig. S1N, 2A–B). To determine whether HNF1A-AS1 regulates gastric cancer cell proliferation in our xenograft experiments, we established xenograft tumor models in nude mice using MKN-45 gastric cancer cells transfected with LV-HNF1A-AS1 or LV-NC (expressing GFP). All nude mice developed xenograft tumors at the injection site, and the xenograft tumors were harvested 5 weeks after injection. We observed that HNF1A-AS1 played an important role in promoting tumor growth (Fig. 1L–M). The xenograft proliferation assay demonstrated that tumor nodules derived from HNF1A-AS1–overexpressing cells grew substantially faster than those in the control group (tumor weight, 9.221 ± 1.494 g vs. 7.330 ± 1.399 g; Fig. 1N; P = 0.0214; tumor volume, 114.7 ± 95.13 mm3 vs. 88.17 ± 73.24 mm3; Fig. 1O; P = 0.046). These findings demonstrate that HNF1A-AS1 initiates the growth and invasion of gastric cancer cells.

Figure 1.

HNF1A-AS1 promotes gastric cancer cell growth and invasion. A, HNF1A-AS1 expression was analyzed by qRT-PCR in gastric cancer tissues (n = 99) and adjacent normal tissues (n = 8). HNF1A-AS1 expression level was normalized to that of GAPDH. B and C, MKN-45 and BGC-823 cell growth was enhanced as evaluated by the EdU assay (B), and HNF1A-AS1 knockdown inhibited cell proliferation activities in both cell lines (C). Three independent experiments were performed, and data are presented as mean ± SD. D–G, The cell growth rates were determined with MTS proliferation assays. Three independent experiments were performed, and data are presented as mean ± SD. H–I, Colony formation assays were used to explore the cell colony formation ability of HNF1A-AS1 and Si-HNF1A-AS-1–transfected cells. Three independent experiments were performed, and data are presented as mean ± SD. J and K, Migration and invasion ability of the BGC-823 cell lines by Transwell assays. Three independent experiments were performed, and data are presented as mean ± SD. L, Five weeks after implantation, the tumors in Nu/Nu mice was measured by an in vivo imaging system. M, The HNF1A-AS1 stably expressing group or the negative control were used for tumorigenesis assay. Five weeks later, the mice were euthanized, and the tumor nodules were harvested. N and O, Tumor growth curves after subcutaneous injection of MKN-45 cells containing a stable overexpression of HNF1A-AS1 or the negative control are shown. The tumor weights (N) and volumes (O) were measured every 7 days after inoculation. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Figure 1.

HNF1A-AS1 promotes gastric cancer cell growth and invasion. A, HNF1A-AS1 expression was analyzed by qRT-PCR in gastric cancer tissues (n = 99) and adjacent normal tissues (n = 8). HNF1A-AS1 expression level was normalized to that of GAPDH. B and C, MKN-45 and BGC-823 cell growth was enhanced as evaluated by the EdU assay (B), and HNF1A-AS1 knockdown inhibited cell proliferation activities in both cell lines (C). Three independent experiments were performed, and data are presented as mean ± SD. D–G, The cell growth rates were determined with MTS proliferation assays. Three independent experiments were performed, and data are presented as mean ± SD. H–I, Colony formation assays were used to explore the cell colony formation ability of HNF1A-AS1 and Si-HNF1A-AS-1–transfected cells. Three independent experiments were performed, and data are presented as mean ± SD. J and K, Migration and invasion ability of the BGC-823 cell lines by Transwell assays. Three independent experiments were performed, and data are presented as mean ± SD. L, Five weeks after implantation, the tumors in Nu/Nu mice was measured by an in vivo imaging system. M, The HNF1A-AS1 stably expressing group or the negative control were used for tumorigenesis assay. Five weeks later, the mice were euthanized, and the tumor nodules were harvested. N and O, Tumor growth curves after subcutaneous injection of MKN-45 cells containing a stable overexpression of HNF1A-AS1 or the negative control are shown. The tumor weights (N) and volumes (O) were measured every 7 days after inoculation. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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HNF1A-AS1 promotes gastric cancer cell-cycle progression

Next, we determined whether HNF1A-AS1 overexpression or knockdown affects the cell cycle or apoptosis in gastric cancer cells. The results indicated that HNF1A-AS1 overexpression promotes the transition from G1 to S phases of the cell cycle (Fig. 2A), whereas suppression of HNF1A-AS1 induced a decrease in the number of cells in the S phase and an unstable number of cells in the G2 phase (Fig. 2B; Supplementary Fig. S2C). Next, we investigated the effects of HNF1A-AS1 on cell apoptosis. Annexin V–FITC assays indicated that there was no difference in the proportion of apoptotic cells between the experimental and control groups following a 48-hour treatment (Fig. 2C–D; Supplementary Fig. S2D). These results showed that HNF1A-AS1–mediated promotion of gastric cancer cell proliferation was due to cell-cycle modulation, not apoptosis.

Figure 2.

The effect of HNF1A-AS1 on gastric cancer cell cycle and apoptosis in vitro. A, Flow cytometry was performed to determine the effects of HNF1A-AS1 on the cell cycle. Significant G1 phase decrease and S phase increase were observed in both cell lines. B, Significant G1 phase increase and S phase decrease were observed in both cell lines. C and D, Apoptosis was analyzed by flow cytometry, which showed that upregulation or downregulation of HNF1A-AS1 expression did not induce apoptosis in comparison with the control cells. Three independent experiments were performed, and data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.

Figure 2.

The effect of HNF1A-AS1 on gastric cancer cell cycle and apoptosis in vitro. A, Flow cytometry was performed to determine the effects of HNF1A-AS1 on the cell cycle. Significant G1 phase decrease and S phase increase were observed in both cell lines. B, Significant G1 phase increase and S phase decrease were observed in both cell lines. C and D, Apoptosis was analyzed by flow cytometry, which showed that upregulation or downregulation of HNF1A-AS1 expression did not induce apoptosis in comparison with the control cells. Three independent experiments were performed, and data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.

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Identification of the proximal promoter regions of HNF1A-AS1

To explore the regulatory mechanisms underlying HNF1A-AS1 upregulation, a detailed promoter analysis was performed. The human HNF1A-AS1 promoter sequence was obtained from the UCSC Genome Browser (http://genome.ucsc.edu/). To identify the proximal promoter region of HNF1A-AS1, the promoter activities of a ∼2,000-base pair (bp) region upstream of the TSS and five deletion constructs were investigated using luciferase activity assays (Fig. 3A). The pGL3-2000/0 plasmid showed strong luciferase activity compared with the luciferase reporter vector pGL3-Basic in 293T cells. No statistically significant difference in luciferase activity between pGL3-2000/0 and the deletion constructs, including pGL3-1014/0, pGL3-503/0, and pGL3-285/0, was observed (Fig. 3B). However, further deletion of up to −187 resulted in a 75% decrease in luciferase activity relative to that of pGL3 −285/0, indicating that the region located between −285 and −187 is the basal promoter of HNF1A-AS1.

Figure 3.

EGR1 activates HNF1A-AS1 transcription. A, Schematic diagram of the HNF1A-AS1 promoter fragments spanning from −2,000/ −1,014/−503/−285/−234/−187 to 0. These promoter fragments were cloned into the upstream of the firefly luciferase reporter gene in the pGL3-basic vector. B, Transcriptional activity analysis of the potential HNF1A-AS1 promoter fragments in 293T cells. C, Luciferase activity assay showed that EGR1 observably increased promoter activities of pGL3 −285/0. D and E, RT-qPCR assay indicated EGR1 promoted the expression level of HNF1A-AS1 in MKN-45 cells (D) and BGC-823 cells (E). F, Schematic diagram of the luciferase reporter construct containing the human HNF1A-AS1 promoter and the mutant construct (H-Mut-1/2/3) containing the basal promoter in which the presumed HNF1A-AS1 binding site was mutated. G, Luciferase activity of the HNF1A-AS1 promoter was decreased when the three presumed HNF1A-AS1 binding site was mutated. H, ChIP-PCR assay showed that EGR1 directly interacted with the EGR1 binding sites within HNF1A-AS1 promoter in MKN-45 cells and BGC-823 cells. A specific strong band of the expected size was detected in the input DNA. The fragment containing the EGR1 binding sites was detected. No band or very weak band was detected in the chromatin complex precipitated by antibody against IgG. H1, H2, and H3, respectively, represent primer that covered the EGR1 binding sites. I and J, ChIP-qPCR analysis indicated higher fold enrichment of promoter amplicons of EGR1 in the anti-EGR1 antibody group than that of the IgG group in MKN-45 cells (I) and BGC-823 cells (J), indicating that EGR1 could directly bind to HNF1A-AS1 promoter. H1, H2, and H3, respectively, represent primers that covered the EGR1 binding sites. K, A significant positive correlation was found between the protein levels of EGR1 and HNF1A-AS1 in gastric cancer tissues. Three independent experiments were performed, and data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Figure 3.

EGR1 activates HNF1A-AS1 transcription. A, Schematic diagram of the HNF1A-AS1 promoter fragments spanning from −2,000/ −1,014/−503/−285/−234/−187 to 0. These promoter fragments were cloned into the upstream of the firefly luciferase reporter gene in the pGL3-basic vector. B, Transcriptional activity analysis of the potential HNF1A-AS1 promoter fragments in 293T cells. C, Luciferase activity assay showed that EGR1 observably increased promoter activities of pGL3 −285/0. D and E, RT-qPCR assay indicated EGR1 promoted the expression level of HNF1A-AS1 in MKN-45 cells (D) and BGC-823 cells (E). F, Schematic diagram of the luciferase reporter construct containing the human HNF1A-AS1 promoter and the mutant construct (H-Mut-1/2/3) containing the basal promoter in which the presumed HNF1A-AS1 binding site was mutated. G, Luciferase activity of the HNF1A-AS1 promoter was decreased when the three presumed HNF1A-AS1 binding site was mutated. H, ChIP-PCR assay showed that EGR1 directly interacted with the EGR1 binding sites within HNF1A-AS1 promoter in MKN-45 cells and BGC-823 cells. A specific strong band of the expected size was detected in the input DNA. The fragment containing the EGR1 binding sites was detected. No band or very weak band was detected in the chromatin complex precipitated by antibody against IgG. H1, H2, and H3, respectively, represent primer that covered the EGR1 binding sites. I and J, ChIP-qPCR analysis indicated higher fold enrichment of promoter amplicons of EGR1 in the anti-EGR1 antibody group than that of the IgG group in MKN-45 cells (I) and BGC-823 cells (J), indicating that EGR1 could directly bind to HNF1A-AS1 promoter. H1, H2, and H3, respectively, represent primers that covered the EGR1 binding sites. K, A significant positive correlation was found between the protein levels of EGR1 and HNF1A-AS1 in gastric cancer tissues. Three independent experiments were performed, and data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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EGR1 activates HNF1A-AS1 transcription

To identify the mechanisms underlying regulation of the basal promoter of HNF1A-AS1, the transcription factor-binding site region from −285 to −187 was analyzed using the JASPER program. Three putative binding sites, including three ELK1-binding sites, six Kruppel-like factor 5 (KLF5)-binding sites, and three EGR1-binding sites were identified. To determine their roles in regulating HNF1A-AS1 transcription, ELK1, KLF5, and EGR1 were, respectively, overexpressed in 293T cells that were transiently transfected with the pGL3-285/0 construct. The results showed that the promoter activities of pGL3-285/0 significantly increased in the EGR1 overexpression group (Fig. 3C). The RT-qPCR assay also showed that EGR1 promotes the expression of HNF1A-AS1 (Fig. 3D and E). These results indicate that the EGR1-binding site is essential for HNF1A-AS1 transcription. To investigate the contribution of the three putative EGR1-binding sites to the regulation of the HNF1A-AS1 promoter, we introduced three point mutations, designated as p (−10 to −23) Luc-EGR1-1, p (−245 to −258) Luc-EGR1-2, and p (−252 to −265) Luc-EGR1-3 (Fig. 3F). The three mutant plasmids were, respectively, transfected into the 293T cells, and luciferase assays were performed. Figure 3G shows that the three mutation vectors, respectively, led to an approximate 90% decrease in promoter activity compared with the wild-type control, indicating that the EGR1-binding site cluster is responsible for HNF1A-AS1 transcription. To determine whether EGR1 specifically interacts with the HNF1A-AS1 promoter, a ChIP assay was performed. Figure 3H shows that the three PCR products were amplified from the DNA fragment that was immunoprecipitated by the anti-EGR1 antibody using primers that covered the three EGR1-binding sites. Additionally, an approximate 10-fold enrichment of the promoter amplicons of HNF1A-AS1 in the three binding sites in gastric cancer cells was observed using the anti-EGR1 antibody (Fig. 3I and J), suggesting that EGR1 could directly bind to the promoter of HNF1A-AS1. In the LinkedOmics database, enhanced EGR1 expression predicted poor patient prognosis (Supplementary Fig. S2E), indicating that EGR1 functions as an oncogene and plays important roles in gastric cancer progression. Furthermore, our results revealed a positive correlation between EGR1 and HNF1A-AS1 expression in human gastric cancer tissue (Fig. 3K; r = 0.3835, P = 0.0364), further supporting the fact that EGR1 positively regulates HNF1A-AS1 expression. Taken together, these findings demonstrate that EGR1 regulates the transcription of HNF1A-AS1 by directly binding to its promoter.

EGR1 enhances gastric cancer cell proliferation

To explore the effects of EGR1 on gastric cancer cell behavior, the MKN-45 and BGC-823 cells were transiently transfected with the pcDNA3.1-EGR1 or pcDNA3.1 plasmid. The qPCR results showed that EGR1 was significantly upregulated in EGR1-transfected cell lines (Supplementary Fig. S2F). The EdU assay demonstrated that EGR1 overexpression significantly promotes gastric cancer cell proliferation compared with the control group (Fig. 4A). Coinciding results of the EdU assay, the MTS assay also showed that EGR1 enhances gastric cancer cell proliferation (Fig. 4B). Moreover, the colony formation assays further verified these results by showing that EGR1 upregulation enhances the number and size of all cell colonies compared with the control group (Fig. 4C). To elucidate the potential mechanisms underlying the growth-promoting effects of EGR1, the cell cycle and apoptosis were evaluated in the MKN-45 and BGC-823 cells by flow cytometry. The results revealed that EGR1 overexpression promotes G1 to S cell-cycle transition (Fig. 4D). Consistent with the results of the MTS and EdU assays, the expression of the cell-cycle–related biomarkers CDK2, CDK4, and cyclin E1 increased, and p21 expression decreased when EGR1 was overexpressed in both gastric cancer cell lines (Fig. 4E). However, the proportion of apoptotic cells was not affected by EGR1 overexpression compared with the control group (Fig. 4F). These findings demonstrate that EGR1-induced gastric cancer cell proliferation may have been due to the promotion of cell-cycle progression.

Figure 4.

EGR1 enhances gastric cancer cell proliferation. A, EdU assay was performed to observe the effect of EGR1 overexpression on cell proliferation. B, Cells were seeded in 96-well plates after transfection with EGR1 overexpression or control vector, and cell number was determined every 24 hours using MTS assays. C, Effect of EGR1 overexpression on the colony formation of MKN-45 and BGC-823 cells. D, Effect of EGR1 overexpression on the cell-cycle progression of both cells. E, The expression levels of CDK2, CDK4, cylcin E1, and P21 in both MKN-45 cells and BGC-823 cells transfected with EGR1 overexpression vector were detected by Western blotting assay. F, Apoptosis was determined by flow cytometry, which showed that EGR1 did not induce apoptosis in comparison with the control cells. Three independent experiments were performed, and data are shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.

Figure 4.

EGR1 enhances gastric cancer cell proliferation. A, EdU assay was performed to observe the effect of EGR1 overexpression on cell proliferation. B, Cells were seeded in 96-well plates after transfection with EGR1 overexpression or control vector, and cell number was determined every 24 hours using MTS assays. C, Effect of EGR1 overexpression on the colony formation of MKN-45 and BGC-823 cells. D, Effect of EGR1 overexpression on the cell-cycle progression of both cells. E, The expression levels of CDK2, CDK4, cylcin E1, and P21 in both MKN-45 cells and BGC-823 cells transfected with EGR1 overexpression vector were detected by Western blotting assay. F, Apoptosis was determined by flow cytometry, which showed that EGR1 did not induce apoptosis in comparison with the control cells. Three independent experiments were performed, and data are shown as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant.

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HNF1A-AS1 enhances the expression of cell-cycle regulators and promotes p21 degradation by CDC34-mediated ubiquitination

To determine how HNF1A-AS1 promotes cell-cycle progression, the expression of the cell-cycle regulators CDK2, CDK4, and cyclin E1 was examined by Western blotting. Among these proteins, the expression of CDK2, CDK4, and cyclin E1 was significantly upregulated in the HNF1A-AS1 overexpression group compared with the control group (Fig. 5A). The CDK inhibitors p21 and p27 are important regulators of the cell cycle. Thus, we hypothesized that HNF1A-AS1 promotes gastric cancer cell-cycle progression via suppression of p21 or p27 expression. As expected, only p21 protein expression decreased when HNF1A-AS1 was overexpressed in the MKN-45 and BGC-823 cells (Fig. 5B–C; Supplementary Fig. S2G), indicating that transcriptional and posttranscriptional levels might be affected by HNF1A-AS1. Then, we performed an RNA polymerase II Inhibitor ActD chase assay to assess the effects of HNF1A-AS1 on p21 mRNA stability. The results showed that p21 mRNA levels were not significantly altered in the HNF1A-AS1 overexpression and knockdown group (Fig. 5D–K). The MKN-45 cells and BGC-823 cells were treated with the protein synthesis inhibitor cycloheximide, which showed that HNF1A-AS1 reduces the stability of the p21 protein (Fig. 5L and M). To determine whether the decrease in p21 protein stability in the HNF1A-AS1 overexpression group was due to an increase in proteasomal-mediated degradation of p21, we conducted experiments with the proteasome inhibitor MG132. MG132 treatment of cells resulted in an increase in p21 protein levels compared with the control group, indicating that the ubiquitin–proteasome pathway is involved in the HNF1A-AS1 degradation of the p21 proteins (Fig. 5N–Q). Consistent with our hypothesis, the ubiquitination of p21 significantly increased in the MKN-45 cells overexpressing HNF1A-AS1 compared with the control group (Fig. 5R). Moreover, the expression of CDK2, CDK4, cyclin E1, and p21 was detected in mouse xenograft tumors (Fig. 5S). HNF1A-AS1 overexpression increased the expression of CDK2, CDK4, and cyclin E1 and decreased the expression of p21, confirming that HNF1A-AS1 promotes cell-cycle progression by inhibiting p21 expression to upregulate CDK2, CDK4, and cyclin E1 expression. Taken together, these results show that HNF1A-AS1 promotes the degradation of p21 through ubiquitination. To further investigate the mechanism by which HNF1A-AS1 regulates p21 ubiquitination, we evaluated cell-cycle–related E3 ubiquitin ligases, including S-phase kinase-associated protein 2 (SKP2) and CDC34. The results showed that the expression of CDC34 but not of SKP2 was significantly upregulated in the HNF1A-AS1 overexpression group (Fig. 5B–C; Supplementary Fig. S2G). To the best of our knowledge, no report has shown whether CDC34, an E2/E3 ubiquitin ligase that ubiquitinates substrates such as p27 (Kip1) (22), Ikappaβalpha (23), and Wee1 (24), ubiquitinates p21. The present study has determined that p21 protein expression decreased in the CDC34 overexpression group (Fig. 6A and B). The results of the ActD chase assay showed that p21 mRNA expression was not altered in the CDC34 overexpression or knockdown group compared with the control group (Fig. 6C–J). Treatment of cells overexpressing CDC34 with cycloheximide led to a significantly shorter p21 half-life than that in the control group (Fig. 6K–N). In contrast, CDC34 increased the levels of the endogenous p21 protein in MKN-45 and BGC-823 cells treated with MG132 (Fig. 6O and P). Based on these findings, we concluded that p21 is a substrate for CDC34. Additionally, Western blot analysis showed that p21 expression decreased in the HNF1A-AS1 overexpression group, but CDC34 knockdown reversed the reduced p21 expression induced by HNF1A-AS1 (Fig. 6Q; Supplementary Fig. S2H), suggesting that CDC34 is a mediator of p21 ubiquitination of p21 protein by HNF1A-AS1. These findings suggest that HNF1A-AS1 enhances CDC34 expression, thereby promoting the ubiquitination and degradation of p21.

Figure 5.

HNF1A-AS1 enhanced the expression of cell-cycle regulators and promoted p21 degradation by ubiquitination. A, The expression levels of CDK2, CDK4, and cylcin E1 in HNF1A-AS1 overexpressed MKN-45 cells and BGC-823 cells were detected by Western blotting assay. B and C, Western blotting assay was used to detect the expression levels of CDC34 and P21 in HNF1A-AS1–overexpressed MKN-45 cells (B) and BGC-823 cells (C). D–G, MKN-45 cells (D and E) and BGC-823 cells (F and G) transfected with HNF1A-AS1 overexpression vector and control cells treated with ActD (5ug/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. H–K, MKN-45 cells (H and I) and BGC-823 cells (J and K) transfected with HNF1A-AS1–targeting shRNA and control cells treated with ActD (5 μg/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. L and M, MKN-45 cells (L) and BGC-823 cells (M) transfected with HNF1A-AS1 overexpression vector and control cells were treated with cycloheximide (CHX; 5 μg/mL) or vehicle for the indicated periods. P21 protein levels were analyzed by Western blotting. N and O, MKN-45 cells (N) and BGC-823 cells (O) transfected with HNF1A-AS1 overexpression vector and control cells were treated with MG132 (5 μmol/L) or vehicle for 24 hours. Cell lysates were analyzed by Western blotting. P and Q, MKN-45 cells (P) and BGC-823 cells (Q) transfected with HNF1A-AS1–targeting shRNA and control cells were treated with MG132 (5 μmol/L) or vehicle for 24 hours. Cell lysates were analyzed by Western blotting. R, HNF1A-AS1 overexpression group or control group were treated with MG-132 (10 μm) for 24 hours. Cell lysates were immunoprecipitated with antibody against P21. The ubiquitination of p21 was notably increased in MKN-45 cells overexpressing HNF1A-AS1 compared with the control group. S, LV-HNF1A-AS1 enhanced the expression of CDK2, CDK4, and cyclin E1 and decreased the expression of p21 in mice tumor xenografts, compared with that of the LV-NC group. Three independent experiments were performed, and data are shown as mean ± SD.

Figure 5.

HNF1A-AS1 enhanced the expression of cell-cycle regulators and promoted p21 degradation by ubiquitination. A, The expression levels of CDK2, CDK4, and cylcin E1 in HNF1A-AS1 overexpressed MKN-45 cells and BGC-823 cells were detected by Western blotting assay. B and C, Western blotting assay was used to detect the expression levels of CDC34 and P21 in HNF1A-AS1–overexpressed MKN-45 cells (B) and BGC-823 cells (C). D–G, MKN-45 cells (D and E) and BGC-823 cells (F and G) transfected with HNF1A-AS1 overexpression vector and control cells treated with ActD (5ug/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. H–K, MKN-45 cells (H and I) and BGC-823 cells (J and K) transfected with HNF1A-AS1–targeting shRNA and control cells treated with ActD (5 μg/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. L and M, MKN-45 cells (L) and BGC-823 cells (M) transfected with HNF1A-AS1 overexpression vector and control cells were treated with cycloheximide (CHX; 5 μg/mL) or vehicle for the indicated periods. P21 protein levels were analyzed by Western blotting. N and O, MKN-45 cells (N) and BGC-823 cells (O) transfected with HNF1A-AS1 overexpression vector and control cells were treated with MG132 (5 μmol/L) or vehicle for 24 hours. Cell lysates were analyzed by Western blotting. P and Q, MKN-45 cells (P) and BGC-823 cells (Q) transfected with HNF1A-AS1–targeting shRNA and control cells were treated with MG132 (5 μmol/L) or vehicle for 24 hours. Cell lysates were analyzed by Western blotting. R, HNF1A-AS1 overexpression group or control group were treated with MG-132 (10 μm) for 24 hours. Cell lysates were immunoprecipitated with antibody against P21. The ubiquitination of p21 was notably increased in MKN-45 cells overexpressing HNF1A-AS1 compared with the control group. S, LV-HNF1A-AS1 enhanced the expression of CDK2, CDK4, and cyclin E1 and decreased the expression of p21 in mice tumor xenografts, compared with that of the LV-NC group. Three independent experiments were performed, and data are shown as mean ± SD.

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Figure 6.

CDC34 promoted p21 degradation. A and B, In CDC34-overexpressed MKN-45 cells and BGC-823 cells, the expression level of P21 was detected by Western blotting. C–F, MKN-45 cells (C and D) and BGC-823 cells (E and F) transfected with CDC34 overexpression vector and control cells treated with ActD (5 μg/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. G–J, MKN-45 cells (G and H) and BGC-823 cells (I and J) transfected with si-CDC34 and control cells treated with ActD (5 μg/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. K–N, MKN-45 cells (K and L) and BGC-823 cells (M and N) transfected with CDC34 overexpression vector and control cells were treated with cycloheximide (CHX; 5 μg/mL) or vehicle for the indicated periods. P21 protein levels were analyzed by Western blotting. O and P, MKN-45 cells (O) and BGC-823 cells (P) transfected with CDC34 overexpression vector and control cells were treated with MG132 (5 μmol/L) or vehicle for 24 hours. Cell lysates were analyzed by Western blotting. Q, Western blotting analysis of the p21 in BGC-823 cells overexpressing HNF1A-AS1 or control cells with or without transient transfection withCDC34 siRNA. Three independent experiments were performed, and data are shown as mean ± SD.

Figure 6.

CDC34 promoted p21 degradation. A and B, In CDC34-overexpressed MKN-45 cells and BGC-823 cells, the expression level of P21 was detected by Western blotting. C–F, MKN-45 cells (C and D) and BGC-823 cells (E and F) transfected with CDC34 overexpression vector and control cells treated with ActD (5 μg/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. G–J, MKN-45 cells (G and H) and BGC-823 cells (I and J) transfected with si-CDC34 and control cells treated with ActD (5 μg/mL) for the indicated periods of time. P21 mRNA levels were analyzed by RT-qPCR. K–N, MKN-45 cells (K and L) and BGC-823 cells (M and N) transfected with CDC34 overexpression vector and control cells were treated with cycloheximide (CHX; 5 μg/mL) or vehicle for the indicated periods. P21 protein levels were analyzed by Western blotting. O and P, MKN-45 cells (O) and BGC-823 cells (P) transfected with CDC34 overexpression vector and control cells were treated with MG132 (5 μmol/L) or vehicle for 24 hours. Cell lysates were analyzed by Western blotting. Q, Western blotting analysis of the p21 in BGC-823 cells overexpressing HNF1A-AS1 or control cells with or without transient transfection withCDC34 siRNA. Three independent experiments were performed, and data are shown as mean ± SD.

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HNF1A-AS1 functions as a ceRNA with miR-661 to upregulate CDC34 expression

Recently, an increasing amount of evidence has demonstrated that lncRNAs can function as ceRNAs to sequester miRNAs, which decreases miRNA expression in the cytoplasm and modulates the derepression of miRNA targets at the level of posttranscriptional regulation (25). To explore the regulatory mechanisms underlying HNF1A-AS1 enhancement of CDC34 expression and to determine whether HNF1A-AS1 works as a ceRNA, miRNAs potentially bound to both HNF1A-AS1 and CDC34 were predicted using the programs RegRNA and Targetscan in combination with miRNA functions reported in the literature (Fig. 7A). MiR-661 and miR-663 exhibited lower luciferase activity in pmirGLO-HNF1A-AS1, indicating that these could directly bind to HNF1A-AS1 through their respective miRNA-binding sites (Fig. 7B–C). Luciferase assays demonstrated that miR-661, not miR-663, suppressed the luciferase activity of pmirGLO-CDC34 (Fig. 7D and E). Subsequently, we found that only miR-661 significantly reduces CDC34 protein levels in both gastric cancer cell lines (Fig. 7F). Our study also suggested that miR-661 inhibits gastric cancer cell migration, invasion (Fig. 7G and H), and proliferation (Fig. 7I). MiRNAs directly downregulate their targets by repressing protein translation by forming RNA-induced silencing complexes (RISC) that contain the argonaute-2 (Ago2) protein. To investigate whether HNF1A-AS1 and CDC34-3′ untranslated region (CDC34-3′UTR) interacts with RISCs, RIP experiments were performed using antibodies against Ago2. Endogenous HNF1A-AS1 and CDC34-3′UTR were specifically enriched in Ago2 immunoprecipitates compared with the control, IgG (Fig. 7J). Additionally, a biotin-labeled pulldown system was used to determine whether miR-661 could pull down HNF1A-AS1 and CDC34. Figure 7K and L shows that the levels of HNF1A-AS1 and CDC34 mRNAs were markedly elevated in the pulldown product isolated from BGC-823 cells following transfection with biotin-labeled miR-661 compared with the control, suggesting that miR-661 interacts with HNF1A-AS1 and CDC34 in a sequence-specific manner. An important corollary of the ceRNA hypothesis is that ceRNA upregulation results in higher target RNA expression and vice versa (26). To assess this, RT-qPCR assays were performed to detect HNF1A-AS1 expression levels in CDC34 overexpression and knockdown gastric cancer cells. We found that HNF1A-AS1 expression was upregulated in the CDC34 overexpression group (Fig. 7M; Supplementary Fig. S2I) and inhibited in the CDC34 knockdown group (Fig. 7N; Supplementary Fig. S2J), indicating a reciprocal effect. Taken together, these results reveal that HNF1A-AS1 functions as a ceRNA for miR-661 and thereby derepresses CDC34 expression and thus provides an additional mechanism for posttranscriptional regulation.

Figure 7.

HNF1A-AS1 functions as a ceRNA with miR-661 to upregulate CDC34 expression. A, A Venn diagram depicting 137 miRNAs that were predicted to target both HNF1A-AS1 and CDC34 by RegRNA 2.0 algorithms. B and C, Luciferase activity was detected using the dual-luciferase assay. The data indicated a decrease in luciferase activity in MKN-45 (B) and BGC-823 (C) cells transfected with PGLO-HNF1A-AS1 and miR-661 or miR-663. D and E, The results indicated a decrease in luciferase activity in MKN-45 (D) and BGC-823 (E) cells transfected with PGLO-CDC34-3′ UTR and miR-661. F, Western blot analyses were performed to confirm the CDC34 gene expression in both cells transfected with miR-661 or miR-663 mimics. G and H, Effects of the miR-661 overexpression on migration and invasion abilities in MKN-45 cells (G) and BGC-823 cells (H). I, EdU assays were performed to detect the effects of miR-661 on cell proliferation in MKN-45 cells (top) and BGC-823 cells (bottom). J, RNA immunoprecipitation with an anti-Ago2 antibody was used to assess endogenous Ago2 binding to HNF1A-AS1 and CDC-34 3′ UTR. IgG was used as the control. K and L, BGC-823 cells were transfected with biotinylated miR-661 (BIO-miR-661) or biotinylated NC (Bio-NC). Forty-eight hours after transfection, cells were collected for a biotin-based pulldown assay. HNF1A-AS1 and CDC34 expression levels were analyzed by RT-qPCR. M and N, RT-qPCR assay was performed to detect HNF1A-AS1 expression in CDC34 upregulating or downregulating BGC-823 cells. O, Proposed functional action of HNF1A-AS1 in modulating gastric cancer tumorigenesis. The transcriptional factor EGR1 activated HNF1A-AS1 expression by directly binding to its promoter, and HNF1A-AS1 functioned as ceRNA by competitively binding to miR-661 to upregulate the expression of CDC34, which is a direct target of miR-661. Then, CDC34-mediated ubiquitination decreased the stability of p21 protein via degradation of P21 protein and subsequently increased the expressions of CDK2, CDK4, cyclin E1 to enhance the proliferation capabilities of gastric cancer cells.

Figure 7.

HNF1A-AS1 functions as a ceRNA with miR-661 to upregulate CDC34 expression. A, A Venn diagram depicting 137 miRNAs that were predicted to target both HNF1A-AS1 and CDC34 by RegRNA 2.0 algorithms. B and C, Luciferase activity was detected using the dual-luciferase assay. The data indicated a decrease in luciferase activity in MKN-45 (B) and BGC-823 (C) cells transfected with PGLO-HNF1A-AS1 and miR-661 or miR-663. D and E, The results indicated a decrease in luciferase activity in MKN-45 (D) and BGC-823 (E) cells transfected with PGLO-CDC34-3′ UTR and miR-661. F, Western blot analyses were performed to confirm the CDC34 gene expression in both cells transfected with miR-661 or miR-663 mimics. G and H, Effects of the miR-661 overexpression on migration and invasion abilities in MKN-45 cells (G) and BGC-823 cells (H). I, EdU assays were performed to detect the effects of miR-661 on cell proliferation in MKN-45 cells (top) and BGC-823 cells (bottom). J, RNA immunoprecipitation with an anti-Ago2 antibody was used to assess endogenous Ago2 binding to HNF1A-AS1 and CDC-34 3′ UTR. IgG was used as the control. K and L, BGC-823 cells were transfected with biotinylated miR-661 (BIO-miR-661) or biotinylated NC (Bio-NC). Forty-eight hours after transfection, cells were collected for a biotin-based pulldown assay. HNF1A-AS1 and CDC34 expression levels were analyzed by RT-qPCR. M and N, RT-qPCR assay was performed to detect HNF1A-AS1 expression in CDC34 upregulating or downregulating BGC-823 cells. O, Proposed functional action of HNF1A-AS1 in modulating gastric cancer tumorigenesis. The transcriptional factor EGR1 activated HNF1A-AS1 expression by directly binding to its promoter, and HNF1A-AS1 functioned as ceRNA by competitively binding to miR-661 to upregulate the expression of CDC34, which is a direct target of miR-661. Then, CDC34-mediated ubiquitination decreased the stability of p21 protein via degradation of P21 protein and subsequently increased the expressions of CDK2, CDK4, cyclin E1 to enhance the proliferation capabilities of gastric cancer cells.

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An increasing number of studies have revealed a key regulatory role of lncRNAs in the carcinogenesis and progression of human cancers (27). However, the potential biological functions and underlying mechanistic details for most lncRNAs in human gastric cancer remain elusive. In the present study, we found that HNF1A-AS1 was markedly upregulated in gastric cancer tissues compared with nontumorous tissues. Using loss-of-function and gain-of-function assays, we demonstrated that HNF1A-AS1 promotes gastric cancer cell proliferation and invasion, suggesting that HNF1A-AS1 may function as an oncogenic lncRNA in gastric cancer. Consistent with our observations, Zhao and colleagues found that HNF1A-AS1 enhances cell proliferation and metastasis in osteosarcoma through activation of the Wnt/β-catenin signaling pathway (17).

Several studies have demonstrated that many lncRNAs contribute to the development and progression of various cancers. However, the regulators involved in the abnormal expression of lncRNAs in cancer cells remain unclear. Extensive evidence has demonstrated that lncRNA expression can be regulated to a certain extent, similar to protein-coding genes. For example, p53 enhances lincRNA-p21 transcription and promotes cell growth arrest and apoptosis (11). EGR1, a Cys2-His2-type zinc-finger transcription factor, belongs to the EGR family and has been implicated in the regulation of cell proliferation (28), migration (29), and apoptosis (30). An increasing amount of evidence has shown that EGR1 is involved in human cancer development and progression, particularly as a tumor promoter. In the present study, we demonstrated that HNF1A-AS1 overexpression in gastric cancer cells could be activated by the transcription factor EGR1 by binding to its promoter region. Previous studies have shown that EGR1 regulates mRNA transcription of protein-coding genes, whereas we first demonstrated that it could activate lncRNA transcription, indicating that the regulation of lncRNA and protein-coding mRNA expression shares the same transcription factor. In addition, the results of the present study show that the effects of EGR1 on gastric cancer cell proliferation may be due, at least in part, to the induction of cell-cycle transition from the G0–G1 to S phases, which coincides with the functions of HNF1A-AS1.

Next, flow cytometry demonstrated that HNF1A-AS1–induced gastric cancer cell proliferation may be associated with cell-cycle transition from the G0–G1 to S phases. To elucidate the mechanism underlying initiation and progression of cancer cell proliferation, it is essential to determine the effects of cell-cycle–related proteins such as cyclin, CDKs, and CKIs on cell-cycle control. The CDK2-cyclin E and CDK4/CDK6-cyclin D complexes are required for cell-cycle transition from the G0–G1 to S phases (31). In the present study, HNF1A-AS1 not only promoted cyclin E1 expression but also enhanced CDK2/CDK4 expression in vitro and in xenograft tumors. The activities of CDKs and cyclins in the G1–S phase could be inhibited by CKIs, particularly p21, which can bind to cyclin and CDK subunits through its characteristic motifs within amino-terminal moieties (32). Our study showed that HNF1A-AS1 also decreases p21 expression.

Moreover, we demonstrated that HNF1A-AS1 could promote CDC34 expression and thus enhance CDC34-mediated ubiquitination and degradation of p21. The CDC34 protein was first identified in yeast as a regulator of the G1 to S phase transition (33). CDC34 belongs to the family of ubiquitin-conjugating enzymes that can catalyze the addition of ubiquitin to other protein substrates. It usually plays an important role in the downregulation of p27, Ikappaβalpha, and Wee1 due to its E2/E3 ubiquitin ligase activity. In this study, we demonstrate that p21 is a novel substrate for human CDC34 in gastric cancer cells that overexpress HNF1A-AS1. Based on these results, we postulate that HNF1A-AS1 increases CDC34 expression, thereby enhancing CDC34-mediated ubiquitination and degradation of p21.

Recent studies have suggested that lncRNAs potentially affect the expression levels of miRNA targets by competing for miRNAs. For instance, lncRNA BC032469 sponges miR-1207-5p by mimicking its binding site, leading to the accumulation of the miR-1207-5p target hTERT in gastric cancer (34). In addition, lncRNA Unigene56159, which is highly upregulated in hepatocellular carcinoma, acts as a “sponge” to downregulate miR-140-5p, leading to the reduction of translational repression of its target gene Slug (35). Thus, we hypothesized that HNF1A-AS1 acts as a ceRNA that upregulates the expression of CDC34. Potential interactions between HNF1A-AS1 and miRNAs were explored using bioinformatics analyses, luciferase assays, RIP assays, and biotin-labeled pulldown assays to confirm the direct binding capabilities of the predicted miRNA responsive elements to the full-length HNF1A-AS1 transcript and CDC34-3′ UTR. The results of the present study demonstrate that HNF1A-AS1 could serve as a ceRNA that sequesters miR-661, thereby protecting the target gene CDC34 from repression and in turn upregulating HNF1A-AS1. The findings of this study indicate that the cross-talk between RNAs, e.g., lncRNA and mRNA, forms a large-scale regulatory network across the transcriptome. In the context of cancer, lncRNAs have been systematically studied as potential tumor oncogenes and suppressors via their ceRNA function. Thus, this ceRNA network could provide opportunities for novel therapeutic regimens. Previous studies have shown that miR-661 plays a tumor-suppressive role in various cancers. For example, miR-661 inhibits the tumorigenicity of invasive breast cancer cells by suppressing a metastasis-related protein, MTA1 (36). Additionally, miR-661 inhibits the proliferation, migration, and invasion abilities of glioma cells by targeting hTERT (37). However, the biological roles of miR-661 in gastric cancer tumorigenesis remain unclear. Our results show that miR-661 suppresses cell proliferation and invasion, suggesting that miR-661 plays a tumor suppressive role in gastric cancer. Thus, HNF1A-AS1 enhances cell proliferation and invasion, which may be partly due to absorbed miR-661 that suppresses the effect of miR-661.

In conclusion, we have identified the core promoter region of HNF1A-AS1 and found that EGR1 plays a role in upregulating HNF1A-AS1 expression by directly binding to the HNF1A-AS1 promoter. In addition, we have demonstrated that HNF1A-AS1 acts as a ceRNA that upregulates the expression of CDC34, which in turn upregulates the level of HNF1A-AS1. Furthermore, we found that EGR1-activated HNF1A-AS1 increases gastric cancer cell proliferation, in which CDK2, CDK4, and cyclin E1 are involved, and decreases the stability of p21 protein by promoting CDC34-mediated ubiquitination and degradation (Fig. 7O). These findings define the importance of HNF1A-AS1 in maintaining cellular proliferation in gastric cancer, and HNF1A-AS1 may be a promising molecular target for the treatment of gastric cancer.

No potential conflicts of interest were disclosed.

Conception and design: H.-T. Liu, L. Liu, P. Gao

Development of methodology: H.-T. Liu, L. Liu, R.-R. Ma, P. Gao

Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): H.-T. Liu, S. Liu, L. Liu, R.-R. Ma, P. Gao

Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis): H.-T. Liu, L. Liu, P. Gao

Writing, review, and/or revision of the manuscript: H.-T. Liu, L. Liu, P. Gao

Administrative, technical, or material support (i.e., reporting or organizing data, constructing databases): H.-T. Liu, S. Liu, L. Liu, P. Gao

This study was supported by the National Natural Science Foundation of China (Grant No. 81672842), The Taishan Scholars Program of Shandong Province (Grant No. ts201511096), and the Key Research and Development Program of Shandong Province (GG201703220053). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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