The current study assessed the anticancer activity of HHP-induced potato patatin on human colorectal adenocarcinoma (HT 29) cell line in vitro. Cell proliferation, apoptosis, necrosis and autophagy were investigated. HHP treatment inhibited HT-29 cell line proliferation in a dose a dependent manner. IC50 values showed antiproliferative activity (59.1%) at 4 mg mL-1 for HHP-induced patatin at 450 MPa. Flow cytometric analysis indicated that HHP-induced patatin at 450 MPa was the potent inducer of in vitro apoptotic cell death and showed significant (p<0.05) accumulation in S-phase followed by patatin pressurized at 550 MPa. Surface charge of patatin decreased with the increase in pressurization up to 350 MPa level, while at 450 MPa zeta potential value increased to (-13 mV) and (-11 mV) respectively. Overall, results indicated that 450 MPa patatin is a strong inhibitor of HT-29 and a potent inducer of apoptotic cell death. Thus, HHP treatment is an effective method for structural modification of patatin which ultimately enhance the anticancer potential against colorectal cancer cells.
Citation Format: Rizwan Elahi, Tai Hua MU. High hydrostatic pressure modified potato patatin induces apoptosis like cell death in human colorectal adenocarcinoma (HT-29) cell line [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 4375.