Gastric cancer was the third leading cause of death worldwide and most prevalent malignant cancer in Korea, comprising 20.8% of all cancers. The Cancer Genome Atlas (TCGA) divided gastric cancer to four subgroups; EBV (Epstein-Barr Virus infected, 9%), MSI (Microsatellite instability, 22%), GS (Genomically stable, 20%), CIN (Chromosomal instability, 50%). We sequenced transcriptomes of 11 gastric cancers associated with EBV infection (EBVaGC) tissues, 11 gastric cancers not associated with EBV infection (EBVnGC) tissues and 22 matched normal gastric mucosa for find unique features of Korean EBVaGC. We compared RNA expression, EBV gene expression, germline mutations, and somatic mutation between EBVaGC and EBVnGC. We found IKBKE gene, which is taking an essential role in regulating antiviral signaling pathways, expressed higher on EBVaGC tumor than EBVnGC tumor (p-value=1.73 x 10-4). While most EBV genes appeared to be similar, BMRF1,2 genes made distinctive patterns compared to previous TCGA report in expression of EBV which integrated into human cells. The BMRF-2 gene is associated with cell-to-cell spread of EBV, which is major infection mechanism of EBV. We found novel germline mutations on PABPC3, PGA3, and C4A,B in almost every samples, which are candidate for Korean specific mutations.
Citation Format: Woochan Lee. EBV associated gastric cancer in Korean [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 4377. doi:10.1158/1538-7445.AM2017-4377