Prostate cancer is the most common cancer in men in the United States and is the second cause of cancer death in men. In Puerto Rico, prostate cancer is the most common type of cancer and the leading cause of cancer death in men. The current available biomarkers are unable to predict malignant outcomes such as recurrence. Thus, there is a critical demand for the development of innovative diagnostic and prognostic tools for the management of prostate cancer. In the current study, we evaluated the biological role of BAT-1 in prostate cancer. Preliminary data from patients who had prostate cancer recurrence identified that HLA-B associated transcript 1 (BAT-1) was down-regulated in patients with prostate cancer recurrence when compared with non-recurrent patients. We down-regulated BAT-1 in PC3 and 22RV1 prostate cancer cell lines using small interfering RNA (siRNA). In vitro assays were performed to measure de-differentiation, proliferation, apoptosis, migration and invasion. Flow cytometry analysis using the cell Viability/Annexin V cell staining kit showed that down-regulation of BAT-1 significantly increased the proliferation of PC3 cells (P<0.05) but decreased apoptosis. Down-regulation of BAT-1 showed no significant change in the expression of vimentin, desmin, and alpha-smooth muscle actin (α-SMA). Wound healing and Boyden assays showed that cells treated with BAT-1 siRNA significantly increased migration and motility at 12h and 24h of PC3 cells when compared to control (P<0.05). Our results showed that BAT-1 down-regulation increased migration and cell motility. Cells also tend to proliferate more, but have reduced apoptosis. These results were expected due to its down-regulation in recurrent prostate cancer patient samples, suggesting that BAT-1 down-regulation promotes aggressiveness in prostate cancer recurrence. A possible mechanism of action for BAT1 is the modulation of cell proliferation and migration.
Citation Format: Aileen M. Garcia-Vargas, Maylein C. Juan-Rivera, Milaris M. Sánchez-Cordero, Maria Sánchez-Vázquez, Krizia Rohena, Magaly Martínez-Ferrer. The biological role of BAT-1 in prostate cancer. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1537.