Penile cancer is a disease with a high morbidity and mortality among developing countries. Although penile cancer is a relatively uncommon cancer in developed countries, its incidence is nearly four times higher in Puerto Rico when compared with other racial and ethnic groups in the United States. Approximately 40% to 45% of penile cancers are HPV-related. The etiology of penile cancers is not completely understood and additional research is necessary to fully delineate the sequence of molecular events involved in both HPV mediated and non-HPV mediated pathways leading to penile cancer. The objective of this study is to determine the genome-wide expression profiles in HPV mediated and non-HPV mediated penile cancer. Penile cancer fresh tissue was obtained from surgically intervened cases. The global gene expression profile was identified and analyzed using the Affymetrix GeneChip® Human Gene 2.0 array. Microarray analysis showed a total of 158 genes differentially expressed between HPV mediated and non-HPV mediated penile cancer. Ingenuity Pathway Analysis (IPA) revealed alterations in the celular movement, cancer, lipid metabolism, carbohydrate metabolism, interleukin, TGF-B, integrin, PI3-kinase and MMP signaling pathways. The microarray results were confirmed by quantitative real-time PCR for some representative genes. Functional and pathway annotation using bioinformatics tools helped to identify specific pathways. Knowledge of the biological pathways and networks of the genes that are significantly expressed or altered are pertinent to understanding the molecular basis of the penile cancer.

Citation Format: María Sánchez-Vázquez, Krizia Rohena-Rivera, Carlos Pérez-Ruiz, Antonio Puras-Báez, Carmen Cadilla, Magaly Martínez-Ferrer. Gene expression analysis of HPV negative and HPV positive penile cancer. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 144.