In this article , which appeared in the July 1, 2002, issue of Cancer Research (1), there appears to be an error in Fig. 3C relating to the expression of cyclin D1 and β-actin mRNA expression in normoxia and hypoxia in HEK293 cells: the loading control bands for β-actin that appear below the cyclin D1 bands are similar to the latter, and in the preparation of the figure the cyclin D1 band images may have been accidentally duplicated and thought to be β-actin bands. Although the identity of the cyclin D1 images has been confirmed recently, the original β-actin images cannot be found. Therefore, the authors have replaced the relevant β-actin bands in Fig. 3C with images for 28S rRNA staining from the gel used for the Northern blot. These confirm equal loading of the relevant RNA samples. The figure legend has been changed to include reference to the 28S rRNA loading controls.
Northern blot analysis of the induction by hypoxia of VHL target genes in cell lines with normal pVHL function. A, in EJ-28 bladder cell line; B, in HBL-100 breast cell line; C, in 293 kidney embryonal cells. Each membrane has been reprobed with VEGF to demonstrate that the culture was grown under hypoxic condition, but for clarity of the figure it is shown only for cyclin D1 in EJ-28 cells. Below each Northern blot, the same membrane reprobed with β-actin for standardization is shown with the exception of cyclin D1 in C, where 28S rRNA is used to demonstrate equal loading.
Northern blot analysis of the induction by hypoxia of VHL target genes in cell lines with normal pVHL function. A, in EJ-28 bladder cell line; B, in HBL-100 breast cell line; C, in 293 kidney embryonal cells. Each membrane has been reprobed with VEGF to demonstrate that the culture was grown under hypoxic condition, but for clarity of the figure it is shown only for cyclin D1 in EJ-28 cells. Below each Northern blot, the same membrane reprobed with β-actin for standardization is shown with the exception of cyclin D1 in C, where 28S rRNA is used to demonstrate equal loading.
These changes do not alter the original scientific conclusions, and the validity of the result remains the same.
The corrected figure and legend appear below. The authors regret the error.
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