MicroRNAs (miRNAs) play important roles as oncogenes or tumor suppressor through modulation of expression of target genes as posttranscriptional regulators. miR-196b has been previously reported dysregulation in multiple tumor types including gastric cancer, colorectal cancer, glioblastoma and breast cancer. However, there is unknown about the role of miR-196b in lung cancer. There has been reported reduction of invasiveness via Homeobox A9 (HOXA9) overexpression in non-small cell lung cancer (NSCLC) cells. We identified an inverse expression between miR-196b and HOXA9 in clinical samples compared to their corresponding normal counterparts. We demonstrated that HOXA9 expression was significantly regulated through alteration of miR-196b expression and miR-196b overexpression or knockdown of HOXA9 induced invasion in NSCLC cells. Moreover, the expression levels of matrix metalloproteinase (MMP) 9 and plasminogen activator, urokinase (uPA) associated with epithelial-mesenchymal transition (EMT) was induced by miR-196b overexpression or knockdown of HOXA9. In addition, the expression level of N-cadherin (Cdh2) was increased by upregulation of miR-196b and knockdown of HOXA9 decreased E-cadherin (Cdh1) expression. Therefore, our findings suggest that miR-196b may be potential therapeutic target in lung cancer.

Citation Format: Ji Woong Son, Seong-Lan Yu, Dong Chul Lee, Shin Yup Lee, Chang Gyo Park, Hoi Young Lee, Jaeku Kang. microRNA-196b induces epithelial-to-mesenchymal transition through targeting HOXA9 in non-small cell lung cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4353. doi:10.1158/1538-7445.AM2014-4353