Purpose: Oral squamous cell carcinoma (OSCC) is prevalent worldwide, and survival in OSCC has not improved significantly in the last few decades. MicroRNAs (miRNAs) have an important regulatory role in human cancer including oral carcinogenesis. MicroRNA 372 and microRNA 373 play as oncogenic and tumour-suppressive functions of between different human maligngncy. This study investigated the microRNA-372 and microRNA 373 expression and their clinical implication in OSCC.
Experimental Design: Fifty patients with primary OSCC were included in the study. Primary cancer cells and matched normal oral epithelium were purified by laser capture microdissection. RNA were extracted from these samples. The expression levels of miR-372 and miR-373 in the tissue of OSCC patients were measured by quantitative RT-PCR.
Results: both miR-372 and miR-373 was up-regulated in OSCC tissue relative to control mucosa. Among different clinical variables, over-expression of miR-372 and miR-373 were associated with nodal metastasis, lymphovascular invasion and poor survival. Multivariate analysis showed that both high miR-372 and miR-373 expression were independent predictors for poor survival in OSCC.
Conclusion: Over expression of miR-372 and miR-373indicate worse survival in OSCC.
Citation Format: Chung Ji Liu, Hsi-Feng Tu, Hui-Wen Cheng, Kuo-Wei Chang. Upregulation of microRNA-372 & 373 associates with cervical lymph node metastasis and poor prognosis of oral carcinomas. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3542. doi:10.1158/1538-7445.AM2014-3542