Tanshinone II-A Inhibits Angiogenesis through Down Regulation of COX-2 in Human Colorectal Cancer

Qi Li*, Li-Hong Zhou, Hua Sui, Yan Wang, Xuan Liu, Ning-Ning Liu, Pei-Hao Yin

Clinic Oncology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai China

*correspondence to Qi Li, Lzwf@hotmail.com

Angiogenesis plays a significant role in colorectal cancer (CRC). cyclooxygenase-2 (COX-2) is involved with multiple aspects of CRC angiogenesis. The aim was to investigate the inhibitory effects of Tan II-A(Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in different individuals respectively in vivo. Then, we assayed tumor weight and volume, evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and VEGF level in vivo. Cell experiment showed marked inhibitory effect of Tan II-A on COX-2 and VEGF of HCT-116 cell lines in a dose-dependent manner. That means Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.

Keywords: Tanshinone II-A - colorectal carcinoma - angiogenesis - cyclooxygenase 2 - vascular endothelial growth factor

Citation Format: Qi Li. Tanshinone II-A inhibits angiogenesis through down regulation of COX-2 in human colorectal cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5101. doi:10.1158/1538-7445.AM2013-5101