Abstract 4981: NADPH oxidase is a novel target of delphinidin to inhibits UVB-induced MMP-1 expression in human dermal fibroblasts

Ultraviolet (UV) induced ROS production is considered a major cause of photoaging via abnormal expression of matrix metalloproteinase (MMP)-1 and NADPH oxidase (NOX) is a key enzyme of endogenous ROS production. Here, we investigated the anti-photoaging effect of delphidin, a major anthocyanin found in berry, beans, and red wine, and its molecular target in UVB-induced MMP-1 expression using primary cultured human dermal fibroblast. Delphinidin inhibited UVB-induced MMP-1 expression in human dermal fibroblasts. UVB-induced MKK4-JNK1/2, MKK3/6-p38, and MEK-ERK1/2 phosphorylation were suppressed by delphinidin. In addition, quantification of ROS stained with the 2′,7′-dichlorofluorescein diacetate (DCF-DA) dye showed that delphinidin significantly inhibited UVB-induced ROS production. Delphinidin significantly inhibited UVB-induced NOX activity. Interestingly, the inhibitory effect of delphinidin on UVB-induced NOX activity was stronger than apocynin, a pharmaceutical NOX inhibitor. Fractioned cell analysis results using a western blot assay showed that delphinidin strongly suppressed UVB-induced P47^phox, a NOX subunit, translocation from the cytosol to the membrane. Delphinidin produced similar effects with apocynin on UVB-induced MMP-1 expression. Pull down assays showed that delphinidin directly bound to P47^phox. Collectively, our results suggest that NOX is a potential target of delphinidin in the suppression of UVB-induced MMP-1 expression in human dermal fibroblasts.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4981. doi:1538-7445.AM2012-4981