The cancer cells which survived hypoxia are known to have aggressive properties and can easily metastasize to other regions, however, the molecular basis of hypoxia-induced changes of cancer cells is not clear. We used a proteomic approach to identify the hypoxia-responsive proteins in 3 ovarian cancer cell lines, KFr13, RMUG-S and ES-2. The cell lines were incubated under lower oxygen concentration (5%) and under normal oxygen concentration (20%) in 48 hours. Even in lower oxygen concentration, the cells survived and increased in number in these 48 hours. The total proteins from the cells were extracted and analysed by 2D-DIGE and MALDI-TOF-MS. We identified the protein, HRP1, whose molecular weight is 71KDa, that all 3 cell lines showed decreased expression under lower oxygen condition compared with normal condition. HRP1 secretion is a growth arrest signal for rat mammary adenocarcionma cells. HRP1 is one of the new candidates for hypoxia-related proteins in ovarian cancer.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3948. doi:1538-7445.AM2012-3948