Ovarian cancer is the leading cause of death from a gynecological malignancy in the United States, yet the genetic mechanisms that contribute to ovarian cancer progression are poorly understood. We have determined that ARID3B, a member of the AT-rich interaction domain (ARID) family of proteins, is overexpressed in ovarian cancer. Therefore, we want to assess if ARID3B plays an important role in ovarian cancer progression. We have demonstrated that there are two ARID3B isoforms: the long form (ARID3BFL at 61kDa) and the short form (ARID3Bsh at 28kDa). We show that the ARID3BFL is mostly found in the nucleus and some is found in the membrane of cells. Furthermore, the short isoform of ARID3B (when overexpressed) is primarily found in cytoplasmic and membrane/mitochondria fractions. Therefore, we hypothesize that by virtue of these unique cellular distributions that ARID3B isoforms elicit different cellular responses. ARID3BFL binds DNA and induces apoptosis in ovarian cancer cell lines while ARID3Bsh does not induce cell death. Further elucidation of the roles of the two ARID3B isoforms will help us understand ovarian cancer progression, cellular response to chemotherapy, and develop prognostic markers and targeted therapies.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2183. doi:1538-7445.AM2012-2183