MicroRNA profiling in isogenic models of cellular transformation involving either breast epithelial cells or fibroblasts reveals that expression of miR-193a is lower in transformed cells than in nontransformed cells. The transcription factors Max and RXRα bind directly to the miR-193a promoter and inhibit miR-193a expression during transformation. miR-193a inhibits cellular transformation by directly targeting the 3′ untranslated regions of PLAU and K-Ras. Interestingly, miR-193a controls anchorage-independent growth in soft agar through K-Ras, whereas it affects invasive growth through PLAU. miR-193a overexpression inhibits the tumorigenicity of developmentally diverse but not all cancer cell types, and it inhibits tumor growth in colon- and breast-derived xenografts. Finally, expression of miR-193a is inversely correlated with PLAU and K-Ras in human colon adenocarcinomas. Thus, a pathway in which Max and RXRα inhibit miR-193a expression, thereby activating the PLAU and K-Ras oncogenes is important for distinct aspects of cellular transformation, as well as tumor growth and colon (and perhaps other types of) cancer. Cancer Res; 71(15); 5144–53. ©2011 AACR.
Comparative transcriptional profiling is a common way to identify genes important for carcinogenesis, and a variety of approaches have been used. Transcriptional profiles are compared among (i) groups of patients with different types of cancer, (ii) primary tumor samples and normal samples from the same patient, (iii) transformed and nontransformed cell lines, and (iv) cell lines that do or do not overexpress an oncogene. Each of these approaches has advantages and disadvantages, but all of them have contributed to our understanding of cellular transformation and cancer.
In previous work, we identified a cancer gene signature on the basis of the identification of genes that are differentially expressed in 2 isogenic models of cellular transformation (1). One model involves nontransformed mammary epithelial cells (MCF-10A; ref. 2) containing ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (3). Treatment of such cells with tamoxifen rapidly induces Src, and morphologic transformation is observed within 24 to 36 hours (4, 5), thereby making it possible to kinetically follow the transition between nontransformed and transformed cells. The other model consists of 3 isogenic cell lines derived from primary fibroblasts in a serial manner (6): EH is immortalized by overexpression of telomerase (hTERT) and exhibits normal morphology; EL also expresses SV40 T antigens and displays an altered morphology but is not transformed; and ELR also expresses oncogenic Ras (H-RasV12), and it is transformed by numerous criteria including tumor formation in mouse xenografts.
The availability of transcriptional profiles for isogenic, but biologically unrelated, models of cellular transformation makes it possible to distinguish between genes that play a relatively general role in transformation as opposed to those affected only by the specific experimental model. Indeed, the 343-gene signature derived from the combined analysis of these isogenic models is highly correlated with a wide variety of human cancers, thereby validating the clinical relevance of our experimental models (1). Of particular interest, this gene signature links cancer with a variety of inflammatory and metabolic diseases, suggesting that a common transcriptional program is involved is phenotypically disparate diseases (1). In the breast epithelial model, transient activation of Src causes an epigenetic switch from nontransformed to transformed cells that involves a positive feedback loop that is associated with a chronic inflammatory state (5, 7).
MicroRNAs (miRNA) play critical roles in cancer and other biological processes by directly interacting with specific mRNAs through base pairing and then inhibiting expression of the target genes through a variety of molecular mechanisms (8–10). In the breast epithelial (ER-Src) model, 29 miRNAs are differentially regulated during the process of transformation, and many of these are important for transformation (5, 7). In particular, downregulation of the Let-7 family and induction of miR-21 and miR-181b are part of the inflammatory feedback loop necessary for the establishment and maintenance of the transformed state. In addition, 22 miRNAs are differentially expressed in cancer stem cells and non–cancer stem cells that arise during the transformation process (11), with the miR-200 family being particularly important for the function of cancer stem cells via repression of polycomb complexes (12–14).
Here, we conduct miRNA profiling in the fibroblast model of cellular transformation and identify 22 differentially regulated miRNAs, 7 of which are similarly regulated in the breast epithelial system. We focus here on miR-193a, which is highly downregulated during transformation of both the ER-Src and fibroblast models. miR-193a is downregulated in oral cancer via DNA hypermethylation (15), and it is poorly expressed in melanomas containing a B-Raf mutation (16). The related miR-193b is 88% identical to miR-193a but is encoded by a separate gene that is regulated differently. miR-193b has been implicated as a tumor suppressor in breast (17), prostate (18), and hepatocellular cancers (19), but high levels of miR-193b are associated with poor prognosis of malignant melanomas (16).
We define a molecular pathway in which expression of miR-193a is directly inhibited by Max and RXRα, thereby leading to increased expression of PLAU and K-Ras, which are direct targets of miR-193a. Furthermore, we show that PLAU and K-Ras have distinct roles in transformation, with PLAU affecting invasiveness, whereas K-Ras affects tumorigenicity. miR-193a acts as a tumor suppressor in developmental diverse cancer cell lines, and miR-193a expression is inversely correlated with both PLAU and K-Ras in human colon adenocarcinomas, suggesting that this pathway is important for some types of human cancer.
Materials and Methods
MCF-10A cells containing the ER-Src fusion protein were grown in DMEM/F12 medium supplemented with 5% donor horse serum (HS), 20 ng/mL epidermal growth factor (EGF), 10 mg/mL insulin, 100 mg/mL hydrocortisone, 1 ng/mL cholera toxin, and 50 units/mL pen/strep, with the addition of puromycin (4, 5). Cells were passaged fewer than 3 months from resuscitation from the originally described cells (4, 5). To induce transformation, the Src oncogene was activated by the addition of 1 mmol/L tamoxifen (Sigma) to confluent cell cultures. BJ fibroblast cell lines were grown in knockout-Dulbecco's modified Eagle's media (KO-DMEM) containing 14% FBS, Medium 199 glutamine, and pen/strep (6). All other cancer cell lines were obtained from American Type Culture Collection and grown in DMEM, 10% FBS, and pen/strep; cells were passaged fewer than 3 months from resuscitation.
MicroRNA expression analysis
Expression levels of 365 microRNAs at various times after tamoxifen addition and from nontransformed and transformed fibroblasts were evaluated with microRNA profiling assays (TLDA human miRNA v1.0) in the Dana Farber Molecular Diagnostics Facility. Validation of these results was conducted using the mirVana qRT-PCR miRNA Detection Kit and qRT-PCR Primer Sets, according to the manufacturer's instructions (Ambion Inc.). RNU48 expression was used as an internal control.
Soft agar colony and invasion assays for the effect of miRNAs on transformation
For the genetic screen to identify miRNAs that act as tumor suppressors, the transformed fibroblast line (ELR) was treated individually with 365 miRNAs (100 nmol/L) for 24 hours. For other experiments, miR-193a (PM11123), miR-193b (PM12383), or an miR-negative control (AM17110) from Ambion Inc. were introduced at 100 nmol/L into a variety of breast and other types of cancer cell lines for 24 hours. In the case of ER-Src cells, tamoxifen was added and cellular transformation was assessed 36 hours later (total time: 60 hours). The soft agar colony and Matrigel invasion assays were conducted as described previously (1, 5). In all cases, experiments were repeated thrice, and the statistical significance was calculated using Student's t test.
For samples generated in this work, RNA was purified by the TRIzol method (Invitrogen), reverse transcribed to generate cDNA, and analyzed by SYBR Green–based real-time PCR, with the level of β-actin used as a loading control. RNAs from colon adenocarcinomas (Origene and BioChain Inc.) were analyzed for levels of PLAU, K-Ras, and miR-193a. Each sample was run in triplicate, and the data represent the mean ± SD. Correlation coefficients between PLAU, K-Ras, and miR-193a expression levels in these colon adenocarcinomas were determined.
3′UTR luciferase assay
Firefly luciferase reporter constructs containing the 3′ untranslated regions (UTR) of PLAU (s207136 from Switchgear Genomics) and K-Ras (HmiT010133 from GeneCopoeia Inc.) were transfected in MCF-10A ER-Src cells along with 100 nmol/L miR-193a or an miRNA control. Cell extracts were prepared 24 hours after transfection, and luciferase activity was measured using the Dual Luciferase Reporter Assay System (Promega).
Cells seeded in 6-well plates were transfected with siRNAs against PLAU (s10610), K-Ras (s15602), RXRα (s12386, s12384), Max (s224030, s8540), or a negative control (AM4611) together with antisense against miR-193a (AM11123), miR-193 (AM12383), or a control antisense (AM17010) using the siPORT NeoFX transfection agent. Oligonucleotides (Ambion Inc.) were used at 50 to 100 nmol/L per well. No cell toxicity was detected because of the transfection agent. The resulting cells were analyzed for transformation or RNA levels as described above. All oligonucleotide transfection experiments were carried out in triplicate.
Chromatin immunoprecipitation was carried out as described previously (5). Briefly, the chromatin fragments, derived from untreated and tamoxifen-treated (36 hours) MCF-10A ER-Src cells, were immunoprecipitated with 6 μg of antibody against Max (ab53570; Abcam Inc.) and RXRα (sc553; Santa Cruz Biotechnology Inc.). DNA extraction was conducted using QIAGEN Purification Kit. The samples were analyzed by quantitative PCR in real time, and the results are presented as the mean ± SD of 3 independent experiments.
MDA-MB-231, HCT-116, and HT-29 cancer cells were injected subcutaneously in the right flank of athymic nude mice (Charles River Laboratories), and tumors were allowed to develop for 10 days (tumor volume ∼ 100 mm3). Mice bearing tumors were randomly distributed in 6 groups (4 mice per group) and treated intraperitoneally with miR-193a (120 nmol/L) or a negative control miR (mixed with liposomes; Altogen Biosystems Inc.; 5010) for 3 cycles (days 10, 15, 20). Tumor growth was monitored every 5 days. Nude mice were maintained in accordance with the Animal Care and Use Committee procedures and guidelines of Tufts University, Medford, MA.
Differentially regulated miRNAs during transformation in the isogenic fibroblast model
In the previous work, we conducted microRNA profiling in an inducible model cellular transformation in breast epithelial cells and identified 29 differentially expressed miRNAs during the transformation process (7). Here, we conducted a similar miRNA profiling analysis in 3 cell isogenic cell lines derived from primary fibroblasts that are immortalized (hTERT), predisposed (hTERT + T antigen), and transformed (hTERT + T antigen + v-Ras). A comparison between immortalized (EH) and transformed (ELR) cell lines identifies 7 miRNAs that are upregulated and 15 miRNAs that are downregulated in the transformed state (Fig. 1A). Most of these miRNAs are not differentially regulated in the predisposed (EL) cell line, although there are some exceptions.
Of the 22 miRNAs differentially regulated in the fibroblast model, 7 are regulated in the same manner during Src-induced transformation in the MCF-10A system (Fig. 1A). All but one of the remaining 15 miRNAs are not regulated in the breast epithelial system (the exceptional miR-132 is regulated in the opposite manner). miR-21 is upregulated in both isogenic models, whereas miR-148b, miR-335, miR-193a, and several members of the Let-7 family are downregulated in both models.
MicroRNAs that inhibit tumorigenicity of transformed fibroblasts
As a genetic screen to identify miRNAs that function as tumor suppressors in the fibroblast model, we individually overexpressed 365 miRNAs in the transformed fibroblasts (ELR line) and examined the effect on tumorigenicity as assayed by colony formation in soft agar. This screen identified 18 miRNAs that inhibit tumorigenicity and 4 miRNAs that slightly enhance tumorigenicity of the transformed fibroblasts (Fig. 1B and Supplementary Fig. S1). All miRNAs downregulated in the transformed cells cause reduced tumorigenicity, and in most cases, the effect is substantial, suggesting that these miRNAs function as tumor suppressors in the fibroblast model. Importantly, miR-148b, miR-335, miR-193a, and the Let-7 family members also inhibit transformation in the breast epithelial system (7), suggesting that these miRNAs function as tumor suppressors in multiple cell types. In this article, we focus on miR-193a because molecular pathways involving this miRNA are essentially unknown.
miR-193a downregulation is important for tumorigenicity and invasive growth in genetically distinct breast cancer cell lines
As shown here (Fig. 1) and elsewhere (7), miR-193a is downregulated during transformation and important for tumorigenicity in both the fibroblast and breast epithelial models. To extend these findings, we analyzed miR-193a and miR-193b expression and function in genetically distinct breast cancer cell lines. In nontransformed MCF-10A cells, miR-193a and miR-193b levels are roughly comparable, with perhaps slightly higher levels for miR-193b (Fig. 2A). In all breast cancer cell lines tested, miR-193a is expressed in lower levels than in immortalized, nontransformed breast cells (Fig. 2A). Furthermore, in all breast cancer cell lines tested, miR-193a overexpression significantly reduces colony formation in soft agar (Fig. 2B). Finally, overexpression of miR-193a in these breast cancer cells reduces the number of invading cells in a Matrigel assay (Fig. 2C). In comparison, levels of miR-193b are more consistent (although not identical) among the cell lines, and the effects on colony formation are less pronounced (Fig. 2A and B). Thus, miR-193a inhibits cellular transformation by limiting anchorage-independent growth and reducing invasiveness.
miR-193a regulates directly the expression of PLAU and K-RAS in breast cancer cells
To investigate how miR-193a exerts its anticancer effects, we generated a list of candidate target genes using 3 prediction algorithms (PicTar, TargetScan, Sanger) on the basis of sequence complementarity to 3′UTRs. Among these candidate target genes, PLAU and K-Ras expression is upregulated, and hence inversely correlated, with miR-193a expression in both transformation models. Furthermore, during the process of cellular transformation in the inducible breast epithelial model, expression of both PLAU (Fig. 3A) and K-Ras (Fig. 3B) gradually increases in parallel to decreasing expression of miR-193a. In the transformed state (36 hours after tamoxifen induction), PLAU is expressed 7-fold higher and K-Ras is expressed 5-fold higher than the level in cells prior to transformation. The inverse expression of miR-193a with both PLAU and K-Ras is consistent with these genes being relevant targets during transformation.
The 3′UTRs of both PLAU and K-Ras have an 8-bp sequence that is perfectly complementary with miR-193a (Fig. 3C), and the PLAU 3′UTR is required for inhibition by the related miR-193b (17). Overexpression of miR-193a in MCF-10A ER-Src cells downregulates the luciferase activity of reporter construct containing either the PLAU or the K-Ras 3′UTR (Fig. 3C). This shows that miR-193a binds directly to these target RNAs and inhibits PLAU and K-Ras expression through 3′UTR base pairing. In addition, overexpression of miR-193a in all breast cancer cell lines tested (which all have low levels of miR-193a; Fig. 2A) significantly inhibits both PLAU and K-Ras expression (Fig. 3D). These results indicate that miR-193a directly inhibits PLAU and K-Ras expression.
miR-193a controls tumorigenicity through K-Ras and invasiveness through PLAU
PLAU is a metastasis-associated protein that supports cell migration and matrix proteases activity (20), and K-Ras is involved in many oncogenic functions such as antiapoptotic activity, angiogenesis, motility, and cell growth (21). To address the roles PLAU and K-Ras play in breast cell transformation, we inhibited the expression of either one of these genes (by siRNA; Supplementary Fig. S2) and miR-193a or miR-193b (by antisense RNA) in MDA-MB-231 breast cancer cells and measured tumorigenicity and invasiveness. As expected, inhibition of miR-193a results in both increased tumorigenicity (Fig. 4A) and increased invasiveness (Fig. 4B), whereas inhibition of miR-193b has little effect. In the context of reduced levels of miR-193a, inhibition of K-Ras reduced tumorigenicity (colony formation in soft agar), whereas inhibition of PLAU had no significant effect (Fig. 4A). In contrast, inhibition of PLAU blocked the increased invasiveness due to inhibition of miR-193a, whereas inhibition of K-Ras has no effect (Fig. 4B). These observations suggest that miR-193a controls the tumorigenicity of breast cancer cells through regulation of K-Ras, whereas it affects their invasiveness through regulation of PLAU.
Max and RXRα bind directly to the miR-193a promoter and inhibit miR-193a expression during transformation
The reduced expression of miR-193a expression during transformation is likely due to a transcription factor(s) that binds to specific DNA sequences in the miR-193a promoter and acts, formally, as a transcriptional repressor. We previously used the Lever algorithm (22) to predict putative transcription factor–binding sites associated with promoters of differentially expressed miRNAs (7). Among the 466 human transcription factor–binding site motifs from the TRANSFAC database and the 272 mouse transcription factor–binding motifs derived by universal protein-binding microarrays, the combination of Max and RXRα ranked among the top combinations for differentially regulated miRNAs including miR-193a.
In accord with these predictions, chromatin immunoprecipitation experiments indicate that Max (Fig. 5A) and RXRα (Fig. 5B) bound to the miR-193a regulatory region in ER-Src–transformed cells; minimal binding was observed in the nontransformed cells. Similarly, both Max and RXRα bind the miR-148a, miR-148b, and miR-181b promoters in transformed but not nontransformed ER-Src cells. Thus, the combination of Max and RXRα binds to the miR-193a and other promoters that are regulated during the transformation process.
Using siRNA-mediated inhibition, we examined the effect of Max and RXRα on transcription of miR-193a at various times during the transformation process (Fig. 5C). Importantly, the decreased level of miR-193a during the late stage of the transformation (2424 and 36 hours after induction) is blocked by inhibition of Max (completely by one siRNA and strongly by another siRNA). In contrast, inhibition of RXRα has a modest, but significant (P < 0.001), effect on the downregulation of miR-193a levels. Similar results for Max and RXRα are observed on the level of the miR-193a primary transcript (Fig. 5D), indicating that repression occurs via a transcriptional mechanism as opposed to a posttranscriptional mechanism involving processing of the miR-193a transcript. Thus, miR-193a transcription is directly repressed by Max and (to a much lesser extent) RXRα at later stages of transformation.
We also examined the effect of Max and RXRα on expression of the other miRNAs whose promoters are bound by these transcription factors (Fig. 5E). As observed for miR-193a, repression of miR-148a and miR-148b in transformed cells (36 hours after tamoxifen treatment) is blocked by inhibition of Max and mildly alleviated by inhibition of RXRα. In contrast, miR-181b is induced in transformed cells, and this induction is blocked by the combined inhibition of Max and RXRα. Individually, both Max and RXRα are important for miR-181b induction, with RXRα having a stronger influence on expression levels (Fig. 5E). Thus, the combination of Max and RXRα directly regulates expression of target miRNA promoters, although regulation can be either positive or negative depending on the target.
miR-193a inhibits tumorigenicity of multiple, but not all, cancer cell types
To examine whether miR-193a affects tumorigenicity of non–breast cancer cell types, we overexpressed miR-193a in a variety of cancer cells from diverse developmental lineages. In addition to its effects on all breast cancer cell lines tested (Fig. 2), miR-193a overexpression dramatically affects tumorigenicity of colorectal (HCT-116 and HT-29) and pancreatic (Panc1) cells as assayed by colony formation in soft agar (Fig. 6A). miR-193a has a modest effect on tumorigenicity of hepatocellular cancer cells (Hep3B), but it has little, if any, effect on prostate (PC3), lung (A549), or cervical (HeLa) cancer cells. Thus, miR-193a appears to act as a tumor suppressor in breast, colorectal, and pancreatic cancer cells, but it has limited effects in a variety of other cancer cell types. Interestingly, the cell lines showing a tumor suppressive effect of miR-193a typically have lower miR-193a expression levels than cell lines not showing this effect (Fig. 6B). This observation suggests that miR-193a is a tumor suppressor in most (and perhaps all) cancer cell types, but that detection of this function occurs only in cell types with reduced miR-193a levels that can be compensated by ectopic expression of miR-193a.
miR-193a acts as a tumor suppressor in mouse xenografts
To examine whether miR-193a acts as tumor suppressor in vivo, we injected nude mice subcutaneously with colorectal (HCT-116 and HT-29) and breast (MDA-MB-231) cancer cells and allowed tumors to develop for 10 days. We then carried out intraperitoneal injection of miR-193a or a negative control miRNA near these small tumors (3 cycles on days 10, 15, 20) and monitored tumor size every 5 days. Over the course of a month, mice treated with miR-193a show reduced tumor growth as compared with mice treated with the control (Fig. 6C). Thus, miR-193a suppresses tumor growth in mouse xenografts generated by breast and colorectal cancer cells, indicating that low levels of miR-193a are required to maintain transformation in these cell types.
miR-193a levels are negatively correlated with PLAU and K-Ras in cancer tissues
In previous studies, we established the cancer relevance of miRNA–target interactions by determining the relationship between their RNA levels in human cancer patients (5, 7, 12). Specifically, an inverse correlation between miRNA and target mRNA levels in a set of cancer patients indicates that the miRNA–target interaction (as opposed to the miRNA or target individually) plays an important role in human cancer. In this regard, there are strong inverse relationships between miR-193a and PLAU RNA levels as well as miR-193a and K-Ras RNA levels in colon cancer patients (Fig. 6D). Thus, miR-193a regulation of PLAU and K-Ras is relevant for at least some types of cancer.
In this work, we describe a gene regulatory pathway involved in cellular transformation (Fig. 7). In this pathway, Max and (to a lesser extent) RXRα, directly binds the miR-193a promoter and inhibits transcription of the gene. The resulting reduction in miR-193a levels leads to increased expression of PLAU and K-Ras, which are direct targets of miR-193a. Increased levels of PLAU and K-Ras contribute to distinct aspects of cellular transformation, with PLAU being important for the invasive growth and K-Ras being important for tumorigenicity.
The combination of Max and RXRα binding motifs is strongly enriched in miRNA promoters whose expression is differentially regulated during transformation (7). Indeed, both Max and RXRα bind to at least 4 miRNA promoters and affect transcription of the target genes. This observation strongly suggests that this combination of transcription factors contributes to the process of cellular transformation by regulating multiple miRNAs and presumably mRNAs. Interestingly, the combination of Max and RXRα can either repress or activate expression of miRNA genes, with Max appearing to be more important for repression and RXRα appearing to be more important for activation. Max binds DNA in association with Myc and other Myc family proteins, and the resulting heteromeric complexes can activate or repress transcription. In this regard, Myc represses transcription of a variety of miRNAs (23, 24). RXRα is a member of a family of nuclear receptors that mediate breast and prostate cancer cell growth (25–28). Our results do not address which Myc family members associate with Max at the miR-193a promoters, nor do they address the molecular mechanism of repression. In addition, the role of Max and RXRα in repressing miR-193a expression has been established only in the breast epithelial (ER-Src) model, and this mechanism differs from the downregulation of miR-193a by DNA methylation in oral cancer (15). Nevertheless, we suspect that Max and RXRα will be important for regulating miR-193a in other breast cell lines and perhaps other cell types.
miR-193a has been suggested to be a tumor suppressor because the gene is inactivated by DNA methylation in oral cancer (15) and poorly expressed in B-Raf melanomas (16). Our observations that miR-193a is downregulated during the cellular transformation process and that miR-193a downregulation is required for transformation in 2 different isogenic models provide direct evidence that miR-193a is a tumor suppressor. Furthermore, the tumor suppressive effects of miR-193a are observed in genetically distinct breast cancer cell lines, multiple colon cancer cell lines, a pancreatic cancer cell line, and in mouse xenografts generated by colon or breast cancer cells, indicating a widespread role of miR-193a in cellular transformation and tumor growth. However, consistent with the general idea that cancer types differ greatly in terms of the genetic and epigenetic events responsible for the diseased state, overexpression of miR-193a does not reduce tumorigenicity in all cancer cell types. In general, the effect of miR-193a is observed in cancer cell lines that have low levels of miR-193a, suggesting that miR-193a overexpression compensates for these low levels and reverses the transformed state. These observations suggest that miR-193a is a tumor suppressor in many, and perhaps all cell types, but that only some cancer cell types are associated with low miR-193a expression.
miR-193a directly targets PLAU and K-Ras and inhibits expression of these genes in genetically distinct breast cancer cell lines. Furthermore, under conditions in which miR-193a levels are reduced via an antisense RNA, PLAU and K-Ras are required, respectively, for invasive growth and tumorigenicity, indicating that these miRNA–target interactions are relevant for distinct aspects of cellular transformation. More importantly, expression of miR-193a is inversely correlated with both PLAU and K-Ras in samples from human colon cancer patients, indicating that the miR-193a interactions with PLAU and K-Ras are relevant for human colon cancer. We suspect that inhibition of PLAU and K-Ras by miR-193a will be relevant for other types of cancer, but this remains to be established directly by analysis of patient samples.
As indicated by their names, miR-193a and miR-193b are related miRNAs that possess the identical 8-nucleotide seed sequence and share 18 of 22 nucleotides (miR-193c is a 21-bp version of miR-193b). miR-193a and miR-193b are encoded by separate genes located on different chromosomes, and their promoter regions are essentially unrelated. As a consequence, miR-193a and miR-193b are not coordinately regulated. In the 2 isogenic models employed here, miR-193a levels are significantly reduced during transformation, whereas miR-193b levels remain essentially unchanged.
The similarity between miR-193a and miR-193b suggests that these miR-193 paralogues have redundant functions, and indeed both paralogues inhibit expression of PLAU. However, in both isogenic models, reduced levels of miR-193a cause transformation, even though miR-193b appears to be expressed at comparable, and perhaps slightly higher, levels than miR-193a, suggesting that these miR-193 paralogues may have some nonredundant functions. Conversely, nonredundant functions are likely to explain why miR-193b can act as a tumor suppressor in cases where miR-193a is also expressed. For example, while miR-193a and miR-193b affect some common targets (e.g., PLAU), there may be other targets that are differentially affected by these miR-193 paralogues. Even in cases like PLAU (or other genes affected by both miR-193a and miR-193b), inhibition by one miR-193 paralogue might be more robust than inhibition by the other paralogue.
The idea that miR-193a and miR-193b have nonredundant functions may explain some previous observations. As shown here, miR-193a appears to play a role in distinguishing nontransformed cells from transformed cells, whereas previous studies suggest that miR-193b is important in distinguishing metastatic from hypermetastatic cancer cells. In addition, while low levels of miR-193a are associated with melanoma, high levels of miR-193b are associated with poor prognosis of this disease (16). This situation in melanoma may mimic the situation in the isogenic models studied here where the transformed state is associated with low levels of miR-193a in the context of substantial levels of miR-193b. Although details of functional distinctions between miR-193a and miR-193b remain to be resolved, our results identify a molecular pathway specifically involving miR-193a that is important for cellular transformation.
Disclosure of Potential Conflicts of Interest
No potential conflicts of interest were disclosed.
The authors thank Philip N. Tsichlis for very kindly providing facilities for carrying out the xenograft experiments.
This work was supported by a grant to K. Struhl from the NIH (CA 107496).
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.