Annona cherimola, known as “cherimoya” was grown as an essential crop in the Incan empire, and was believed to prevent disease. Indigenous to Peru and Ecuador, this “pearl of the Andes” is consumed mainly for its exceptional taste. American writer Mark Twain called the cherimoya “the most delicious fruit known to men.” Recent interest in this fruit stems from its high concentration of acetogenins, which are bioactive polyketides. Recent research has accredited beneficial effects to acetogenins, including the exhibition of cytotoxic and anti-tumorous activity, particularly in mammary, pancreatic and colon cancer cells (Cheng et. al 1998). This project explores the health benefits of cherimoya consumption by examining the antioxidant effect of its components, including: skin, flesh and juice. These components were analyzed for antioxidant concentrations using the Oxygen Radical Absorbance Capacity (ORAC) assay.

A cellular model was also established that evaluated the antioxidant capacity of cherimoya components in the presence of Raji cells, a Burkitt lymphoma cell line, and HT-29 cells, a colon cancer cell line. After incubation with the cherimoya components, cell lysate samples were assessed for antioxidant capacity using the ORAC assay. Results for Raji cell lysate exposed to media, juice, skin and flesh were as follows: 886 Trolox Equivalents per Liter (TE/L), 1238 TE/L, 1018 TE/L, and 1045 TE/L respectively. Results for HT-29 cell lysate exposed to media, juice, skin and flesh were: 294 TE/L, 924 TE/L, 505 TE/L and 627 TE/L respectively. These data shows that cells treated with cherimoya juice exhibited a higher antioxidant uptake.

An alternate cellular model was established in which Raji cells and HT-29 cells were pre-exposed to oxidant AAPH, 2,2’-azobis-(2-amidinopropane) dihydrochloride, and then treated with cherimoya juice, skin and flesh. Results for Raji cells exposed to AAPH and then treated with media, juice, skin and flesh were as follows: 1054 TE/L, 1363 TE/L, 1183 TE/L, and 1254 TE/L respectively. Results for HT-29 cells exposed to AAPH and then treated with media, juice, skin and flesh were: 1075 TE/L, 1528 TE/L, 1350 TE/L and 1374 TE/L respectively. These data indicated that when cells were pre-exposed to an oxidant, thus simulating cells under oxidative stress, antioxidant cell uptake increased for both cell lines. These results suggest that cells can sense oxidative stress in their environment and can increase their ability to uptake available antioxidants.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1900.